Sandwich ELISA analysis of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) was performed using the Thermo Scientific Human IL-10 Colorimetric ELISA kit (Product # EHIL10) by loading 50µl per well of Human IL-10 recombinant protein (Product # SIL10) in triplicate at 600, 240, 96, 38.4, 15.36, and 0pg/ml across a 2µg/ml Rat anti-Human IL-10 (Product # M010) pre-coated plate and incubated for 1.5 hours at room temperature. The plate was washed and incubated with 50µl per well of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) in triplicate at 0.3µg/ml for 1.5 hours in conjunction with standards at room temperature. The plate was washed and incubated with 100µl per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:18,000 for 15 minutes at room temperature. The plate was washed and incubated with 100µl per well of TMB Substrate Solution (Product # N301) for 20 minutes at room temperature in the dark. The reaction was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant human IL-10 (COS cell-derived)|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
M011B targets IL-10 in ELISA and Western blot applications and shows reactivity with human samples.
The M011B immunogen is recombinant human IL-10 (COS cell-derived). The original parent clone is 12G8 and has been subcloned as 2553.2.4.
M011B detects IL-10 which has a predicted molecular weight of approximately 19 kDa.
The M011B IL10 antibody (biotinylated conjugate of clone 12G8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M010 (clone 9D7). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M011B (clone 12G8) and antibody M010 (clone 9D7) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular and functional characterization of allogantigen-specific anergic T cells suitable for cell therapy.
M011B was used in ELISA to characterize allogantigen-specific anergic T cells
|Bacchetta R,Gregori S,Serafini G,Sartirana C,Schulz U,Zino E,Tomiuk S,Jansen U,Ponzoni M,Paties CT,Fleischhauer K,Roncarolo MG||Haematologica (95:2134)||2010|
Hepatitis C virus modulates human monocyte-derived dendritic cells.
M011B was used in ELISA to study the effect of hepatitis C virus on human monocyte-derived dendritic cells
|Eksioglu EA,Bess JR,Zhu H,Xu Y,Dong HJ,Elyar J,Nelson DR,Liu C||Journal of viral hepatitis (17:757)||2010|
Methods for in vitro generation of human type 1 regulatory T cells.
M011B was used in ELISA to study cytokine production profile of Tr1 cell lines
|Gregori S,Roncarolo MG,Bacchetta R||Methods in molecular biology (Clifton, N.J.) (677:31)||2010|
Type 1 regulatory T cells are associated with persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia.
M011B was used in ELISA to investigate the role of type 1 regulatory T cells during persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia
|Serafini G,Andreani M,Testi M,Battarra M,Bontadini A,Biral E,Fleischhauer K,Marktel S,Lucarelli G,Roncarolo MG,Bacchetta R||Haematologica (94:1415)||2009|
Anti-ribosomal phosphoprotein autoantibody triggers interleukin-10 overproduction via phosphatidylinositol 3-kinase-dependent signalling pathways in lipopolysaccharide-activated macrophages.
M011B was used in ELISA to investigate the mechanism of interleukin 10 overproduction induced by anti-ribosomal phosphoprotein autoantibody in lipopolysaccharide-activated macrophages
|Lee TP,Leu SJ,Huang JC,Song YC,Jhou RS,Tang SJ,Sun KH||Immunology (127:91)||2009|
Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women.
M011B was used in ELISA to investigate the reactivity of CD4+ lymphocytes specific to hepatitis C virus in children born from HCV-infected women
|Della Bella S,Riva A,Tanzi E,Nicola S,Amendola A,Vecchi L,Nebbia G,Longhi R,Zanetti AR,Villa ML||Journal of hepatology (43:394)||2005|
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
M011B was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients
|Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M||Rheumatology (Oxford, England) (43:960)||2004|
Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients.
M011B was used in ELISA to investigate the generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients
|Solomon S,Masilamani M,Mohanty S,Schwab JE,Boneberg EM,Illges H||Rheumatology international (24:71)||2004|
Altered maturation of peripheral blood dendritic cells in patients with breast cancer.
M011B was used in ELISA to characterize the peripheral blood dendritic cell maturation in breast cancer patients
|Della Bella S,Gennaro M,Vaccari M,Ferraris C,Nicola S,Riva A,Clerici M,Greco M,Villa ML||British journal of cancer (89:1463)||2003|
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
M011B was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients
|Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M||Transplantation proceedings (35:1523)||2003|
Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.
M011B was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients
|Loffreda S,Muratori P,Muratori L,Mele L,Bianchi FB,Lenzi M||Journal of hepatology (38:230)||2003|
Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.
M011B was used in ELISA to investigate the mechanism for the effect of Leishmania on interleukin 12 p70 secretion
|McDowell MA,Marovich M,Lira R,Braun M,Sacks D||Infection and immunity (70:3994)||2002|
|Not Applicable||Not Cited||
Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.
M011B was used in ELISA to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors
|Suwa T,Hogg JC,Vincent R,Mukae H,Fujii T,van Eeden SF||Experimental lung research (28:1)||2002|
The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.
M011B was used in ELISA to study the interleukin levels in the mice with various diets
|Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA||Surgery (131:59)||2002|
Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production.
M011B was used in ELISA to study the mechanism for the effect of prostaglandins on the maturation and function of monocyte-derived dendritic cells
|Whittaker DS,Bahjat KS,Moldawer LL,Clare-Salzler MJ||Journal of immunology (Baltimore, Md. : 1950) (165:4298)||2000|
Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression.
M011B was used in ELISA to measure the interleukin-10 and interleukin-4 level in Tanzanian children with malaria
|Anstey NM,Weinberg JB,Hassanali MY,Mwaikambo ED,Manyenga D,Misukonis MA,Arnelle DR,Hollis D,McDonald MI,Granger DL||The Journal of experimental medicine (184:557)||1996|
Contribution of IL-1, CD14, and CD13 in the increased IL-6 production induced by in vitro monocyte-synoviocyte interactions.
M011B was used in ELISA to investigate the effect of interleukin 1, CD14, and CD13 on the expression of interleukin 6 produced by monocytes and synoviocytes in vitro
|Chomarat P,Rissoan MC,Pin JJ,Banchereau J,Miossec P||Journal of immunology (Baltimore, Md. : 1950) (155:3645)||1995|
Mistletoe lectin-1 increases tumor necrosis factor-alpha release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production.
M011B was used in flow cytometry to investigate the mechanims for the effect of mistletoe lectin-1 on tumor necrosis factor-alpha release
|Boneberg EM,Hartung T||The Journal of pharmacology and experimental therapeutics (298:996)||2001|