Sandwich ELISA analysis of mouse IL-10 was performed using a Mouse IL-10 Colorimetric ELISA kit (Product # EM2IL10) by loading 50 ul per well of Mouse IL-10 Recombinant Protein (Product # SMIL10) in triplicate at 3000, 1000, 333, 111, 37, and 0 pg/ml across a 1 ug/ml anti-mouse IL-10 (Product # MM011) pre-coated plate and incubating for 3 hours at room temperature. The plate was washed, then incubated with 50 ul per well of an anti-mouse IL-10 biotinylated antibody in triplicate at 1 ug/ml for 1 hour at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) in all test wells at 1:40 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Recombinant mouse IL-10|
|Purification||Caprylic acid precipitation|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 8 publications below|
MM011 targets IL-10 in ELISA applications and shows reactivity with mouse samples.
The MM011 immunogen is recombinant mouse IL-10.
MM011 detects IL-10 which has a predicted molecular weight of approximately 19 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product is a Low Endotoxin formulation.
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Trypanosoma cruzi trans-sialidase is a T cell-independent B cell mitogen and an inducer of non-specific Ig secretion.
MM011 was used in ELISA to study the role of Trypanosoma cruzi trans-sialidase in B cell proliferation and Ig secretion
|Gao W,Wortis HH,Pereira MA||International immunology (14:299)||2002|
Orally administered decoction of Kampo (Japanese herbal) medicine, "Juzen-Taiho-To" modulates cytokine secretion and induces NKT cells in mouse liver.
MM011 was used in ELISA to investigate the effect of Kampo medicine "Juzen-Taiho-To" administered orally for cytokine secretion and NKT cell induction in mouse liver
|Matsumoto T,Sakurai MH,Kiyohara H,Yamada H||Immunopharmacology (46:149)||2000|
Immunogenicity and arthritogenicity of recombinant CB10 in B10.RIII mice.
MM011 was used in ELISA to investigate the role of CD10 in immunogenicity and arthritogenicity
|Tang B,Brand DD,Chiang TM,Stuart JM,Kang AH,Myers LK||Journal of immunology (Baltimore, Md. : 1950) (164:481)||2000|
IL-4 enhances IL-10 gene expression in murine Th2 cells in the absence of TCR engagement.
MM011 was used in ELISA to study the effect of interleukin 4 on interleukin 10 expression and its mechanism
|Schmidt-Weber CB,Alexander SI,Henault LE,James L,Lichtman AH||Journal of immunology (Baltimore, Md. : 1950) (162:238)||1999|
T cell receptor specificities of Toxoplasma gondii-reactive mouse CD4+ T lymphocytes and Th1 clones.
MM011 was used in ELISA to investigate the specificities of Toxoplasma gondii-reactive mouse CD4+ T lymphocytes and Th1 clones
|Reichmann G,Dlugonska H,Fischer HG||Medical microbiology and immunology (186:25)||1997|
Local release of IL-10 by transfected mouse mammary adenocarcinoma cells does not suppress but enhances antitumor reaction and elicits a strong cytotoxic lymphocyte and antibody-dependent immune memory.
MM011 was used in ELISA to investigate the effect of interleukin 10 released from transfected mouse mammary adenocarcinoma cells on antitumor reaction, cytotoxic lymphocyte production, and antibody-dependent immune memory
|Giovarelli M,Musiani P,Modesti A,Dellabona P,Casorati G,Allione A,Consalvo M,Cavallo F,di Pierro F,De Giovanni C||Journal of immunology (Baltimore, Md. : 1950) (155:3112)||1995|
Systemic cytokine profiles in BALB/c mice immunized with trivalent influenza vaccine containing MF59 oil emulsion and other advanced adjuvants.
MM011 was used in ELISA to investigate the profiles of cytokines in BALB/c mice immunized with trivalent influenza vaccine containing MF59 oil emulsion and other advanced adjuvants
|Valensi JP,Carlson JR,Van Nest GA||Journal of immunology (Baltimore, Md. : 1950) (153:4029)||1994|
Mechanism involved in the systemic suppression of antigen-presenting cell function by UV irradiation. Keratinocyte-derived IL-10 modulates antigen-presenting cell function of splenic adherent cells.
MM011 was used in ELISA to investigate the mechanism for the systemic suppression of antigen-presenting cell function by UV irradiation
|Ullrich SE||Journal of immunology (Baltimore, Md. : 1950) (152:3410)||1994|