|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human IL-10.|
|Storage buffer||PBS, pH 7.5, with 5mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 7 publications below|
This product is recommended as a detection antibody in Sandwich ELISA applications.
Interleukin 10 (IL-10) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine.
AHC7109 was used in ELISA to establish a microfluidic method to analyze single cell secretions
|Konry T,Dominguez-Villar M,Baecher-Allan C,Hafler DA,Yarmush ML||Biosensors and bioelectronics (26:2707)||2011|
Biological inactivation and impaired detection of IL-10 by suramin.
AHC7109 was used in ELISA to assess antibodies used in IL-0 for enzyme-linked immunosorbent assay.
|Thom F,Stockhausen H,Germann T,Frosch S,Wade EJ||Journal of immunological methods (299:91)||2005|
|Non-human primate||0.8 ug/ml||
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
AHC7109 was used in ELISA to test if repeated exposure to Schistosoma mansoni stimulates hepatic fibrosis in olive baboons.
|Farah IO,Mola PW,Kariuki TM,Nyindo M,Blanton RE,King CL||Journal of immunology (Baltimore, Md. : 1950) (164:5337)||2000|
Selective suppression of IL-12 production by chemoattractants.
AHC7109 was used in ELISA to determine the ability of chemoattractants to affect IL-2 production by human monocytes and dendritic cells.
|Braun MC,Lahey E,Kelsall BL||Journal of immunology (Baltimore, Md. : 1950) (164:3009)||2000|
|Non-human primate||0.8 ug/ml||
Cytokine control of the granulomatous response in Schistosoma mansoni-infected baboons: role of exposure and treatment.
AHC7109 was used in ELISA to test if variations in exposure and treatment contribute to the heterogeneity in immunity and granuloma-induced pathology in schistosomiasis.
|Mola PW,Farah IO,Kariuki TM,Nyindo M,Blanton RE,King CL||Infection and immunity (67:6565)||1999|
|Rhesus monkey||Not Cited||
Protective immunity using recombinant human IL-12 and alum as adjuvants in a primate model of cutaneous leishmaniasis.
AHC7109 was used in ELISA to use rhIL-12 and alum as adjuvants in primate model of cutaneous leishmaniasis to test if the killed pathogen is a sufficient to generate protective antibodies.
|Kenney RT,Sacks DL,Sypek JP,Vilela L,Gam AA,Evans-Davis K||Journal of immunology (Baltimore, Md. : 1950) (163:4481)||1999|
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
AHC7109 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.
|Braun MC,He J,Wu CY,Kelsall BL||The Journal of experimental medicine (189:541)||1999|