|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Human IL-10 expressed by COS cells.|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/100|
|Western Blot (WB)||1/50-1/250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is human tonsil.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bilberry-Derived Anthocyanins Modulate Cytokine Expression in the Intestine of Patients with Ulcerative Colitis.
MA1-82664 was used in immunohistochemistry - paraffin section to assess modulation of cytokine expression in the intestine of patients with ulcerative colitis by bilberry-derived anthocyanins
|Roth S,Spalinger MR,Gottier C,Biedermann L,Zeitz J,Lang S,Weber A,Rogler G,Scharl M||PloS one (11:null)||2016|
Gliomas promote immunosuppression through induction of B7-H1 expression in tumor-associated macrophages.
MA1-82664 was used in immunohistochemistry to study the role of B7-H1 upregulation in tumor-infiltrating macrophages in the mechanism by which gliomas induce immunosuppression
|Bloch O,Crane CA,Kaur R,Safaee M,Rutkowski MJ,Parsa AT||Clinical cancer research : an official journal of the American Association for Cancer Research (19:3165)||2013|
|Not Applicable||Not Cited||
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
MA1-82664 was used in ELISA to compare the effect of different immunosuppressive regimens on cytokine expression in the grafted lung
|Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M||Transplantation proceedings (35:1523)||2003|
|Not Applicable||Not Cited||
Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers.
MA1-82664 was used in ELISA to study IL-10 in the peritoneal cavity of ovarian cancer bearing patients
|Gotlieb WH,Abrams JS,Watson JM,Velu TJ,Berek JS,Martínez-Maza O||Cytokine (4:385)||1992|