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Invitrogen
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1 µg/mL | - |
Flow Cytometry (Flow) |
- | View 5 publications 5 publications |
ELISA (ELISA) |
0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture) | View 1 publication 1 publication |
Neutralization (Neu) |
0.005-0.015 µg/mL | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Mouse |
Published species |
Not Applicable |
Host/Isotype |
Rat / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
JES052A5 |
Immunogen |
E. coli-derived recombinant mouse IL-10 |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
0.5 mg/mL |
Purification |
Protein A/G |
Storage buffer |
PBS with 5% trehalose |
Contains |
No Preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2609275 |
In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL-10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL-10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1
Gene Aliases: CSIF; Il-10; Il10
UniProt ID: (Mouse) P18893
Entrez Gene ID: (Mouse) 16153
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