Invitrogen

IL-10 Monoclonal Antibody (JES052A5)

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated. View Details

7 References

View all (61) IL-10 antibodies

Datasheet

Datasheet

Product Image Gallery

IL-10 Antibody in Neutralization (Neu) — Neutralization assay of recombinant mouse IL-10 stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by recombinant mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-mouse IL-10 Monoclonal Antibody (Product # MA5-23796). The ND50 is typically 0.005-0.015 µg/mL.

IL-10 Antibody in Neutralization — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-10 is shown by decrease in RFU (measure of proliferation) with increasing concentrations of IL-10 monoclonal antibody (MA5-23796). {Neu}

Product Details

Tested Applications

Dilution

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

Neutralization (Neu)

0.005-0.015 µg/mL

Western Blot (WB)

1 µg/mL

Published Applications

Neutralization (Neu)

See 1 publication below

Flow Cytometry (Flow)

See 5 publications below

ELISA (ELISA)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host / Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

JES052A5

Immunogen

E. coli-derived recombinant mouse IL-10

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2609275

Product Specific Information

In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL-10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL-10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.