Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™

Product Image Gallery

IL-10 Antibody in Flow Cytometry (Flow) — Figure 5 IL-10 + lymphocyte assay. The production of IL-10 + lymphocyte subsets by flow cytometry. (A) Both Cysticercus cellulosae ESA and CA induced the production of IL-10 + lymphocyte. (B) CD4 + T lymphocyte was the main source Cysticercus cellulosae ESA and CA-induced IL-10 expression. (C) In the presence or absence of LPS, Cysticercus cellulosae ESA and CA significantly inhibited the expression frequency of CD8 + IL-10 + lymphocyte. (D) Cysticercus cellulosae ESA could be induced the expression of CD4 - CD8 - IL-10 + cell under the presence or absence of LPS. While, CA only decreased the expression in the presence of LPS. (E) Similar to (C) , the production of IL-10 in CD4 + CD8 + lymphocyte was inhibited by Cysticercus cellulosae ESA. All dates were shown as the means +- SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

Product Details

12-7108-41

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 14 publications 14 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES3-9D7
Conjugate	PE Alexa Fluor 488 Brilliant UV 737 eFluor 450 eFluor 660 Functional Grade PE-Cyanine7 PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10717511

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: JES3-9D7 has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Loss of Claudin-3 Impairs Hepatic Metabolism, Biliary Barrier Function, and Cell Proliferation in the Murine Liver.

Cellular and molecular gastroenterology and hepatology

Baier FA,Sánchez-Taltavull D,Yarahmadov T,Castellà CG,Jebbawi F,Keogh A,Tombolini R,Odriozola A,Dias MC,Deutsch U,Furuse M,Engelhardt B,Zuber B,Odermatt A,Candinas D,Stroka D

12-7108-41 was used in Flow cytometry/Cell sorting to investigate the functional contribution of individual tight junction proteins to barrier and metabolic homeostasis.

Thu Jan 27 00:00:00 EST 2022

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 12-7108-41) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 12-7108-41) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 12-7108-41) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IL-10 monoclonal antibody (Product # 12-7108-41) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis.

Nature communications

Sun M,Wu W,Chen L,Yang W,Huang X,Ma C,Chen F,Xiao Y,Zhao Y,Ma C,Yao S,Carpio VH,Dann SM,Zhao Q,Liu Z,Cong Y

12-7108-41 was used in Flow Cytometry to elucidate the various roles of gut microbiota-derived short-chain fatty acids in the maintenance of intestinal homeostasis.

Mon Sep 03 00:00:00 EDT 2018

Renal Transplant Recipients Treated with Calcineurin-Inhibitors Lack Circulating Immature Transitional CD19+CD24hiCD38hi Regulatory B-Lymphocytes.

PloS one

Tebbe B,Wilde B,Ye Z,Wang J,Wang X,Jian F,Dolff S,Schedlowski M,Hoyer PF,Kribben A,Witzke O,Hoerning A

12-7108 was used in Flow cytometry/Cell sorting to analyse the effect of calcineurin inhibition on peripherally circulating regulatory B cells in renal transplant recipients receiving cyclosporine A or tacrolimus.

Mon Aug 29 00:00:00 EDT 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 12-7108-41) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 12-7108-41) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors.

BMC hematology

Silveira AC,Santana MA,Ribeiro IG,Chaves DG,Martins-Filho OA

12-7108 was used in Flow cytometry/Cell sorting to understand immune response to FVIII in patients with HA who were either positive [HAα-FVIII(+)] or negative [HAα-FVIII(-)] for inhibitors.

Thu Feb 05 00:00:00 EST 2015

CD26-mediated induction of EGR2 and IL-10 as potential regulatory mechanism for CD26 costimulatory pathway.

Journal of immunology (Baltimore, Md. : 1950)

Hatano R,Ohnuma K,Otsuka H,Komiya E,Taki I,Iwata S,Dang NH,Okumura K,Morimoto C

12-7108 was used in Flow cytometry/Cell sorting to investigate negative feedback mechanisms to regulate CD26-mediated T cell activation.

Sun Feb 01 00:00:00 EST 2015

Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

12-7108 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

Thu Jan 01 00:00:00 EST 2015

The anti-idiotypic antibody 1F7 stimulates monocyte interleukin-10 production and induces endotoxin tolerance.

Journal of inflammation (London, England)

Davtyan TK,Poghosyan DA,Sukiasyan AG,Grant MD

12-7108 was used in Flow cytometry/Cell sorting to investigate the direct effects of the 1F7 mAb on peripheral blood mononuclear cells.

Fri Apr 05 00:00:00 EDT 2013

Differential IL-7 responses in developing human thymocytes.

Human immunology

Marino JH,Tan C,Taylor AA,Bentley C,Van De Wiele CJ,Ranne R,Paliotta M,Broughan TA,Teague TK

12-7108 was used in Flow cytometry/Cell sorting to assess IL-7 receptor expression and IL-7 signalling in human thymocytes.

Thu Apr 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

UniProt ID: (Human) P22301

Entrez Gene ID: (Human) 3586

Function(s)

Process(es)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™

Product Details

12-7108-41

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!