Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), PE-Cyanine7, eBioscience™

Product Image Gallery

IL-10 Antibody in Flow Cytometry (Flow) — Normal human peripheral blood mononuclear cells were cultured for 6 days under Th2-polarizing conditions. The cells were unstimulated (left) or restimulated (right) with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) then intracellularly stained with Anti-Human CD4 PE (Product # 12-0049-42) and Anti-Human IL-10 PE-Cyanine7 (Product # 25-7108-42) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

Product Details

25-7108-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.06 µg)/test	View 10 publications 10 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse, Rhesus monkey
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES3-9D7
Conjugate	PE-Cyanine7 Alexa Fluor 488 Brilliant UV 737 eFluor 450 eFluor 660 Functional Grade PE PerCP-eFluor 710 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2573524

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Altered T-Cell Subsets are Associated with Dysregulated Cytokine Secretion of CD4⁺ T Cells During HIV Infection.

Journal of inflammation research

Wang D,Jiang Y,Song Y,Zeng Y,Li C,Wang X,Liu Y,Xiao J,Kong Y,Zhao H

25-7108-42 was used in Flow Cytometry to suggest the importance of therapies that can preserve and/or enhance the function of CD4+ T cells in strategies of HIV remission.

Wed Apr 27 00:00:00 EDT 2022

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 25-7108-42) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 25-7108-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 25-7108-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IL-10 monoclonal antibody (Product # 25-7108-42) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

25-7108 was used in Flow cytometry/Cell sorting to provide evidence that chloroquine exerts its antitumour effect through its immune-regulating function, suggesting a potential new macrophage-based tumour immunotherapeutic modality.

Wed Feb 28 00:00:00 EST 2018

Pre-existing neutralizing antibody mitigates B cell dysregulation and enhances the Env-specific antibody response in SHIV-infected rhesus macaques.

PloS one

Jaworski JP,Bryk P,Brower Z,Zheng B,Hessell AJ,Rosenberg AF,Wu TT,Sanz I,Keefer MC,Haigwood NL,Kobie JJ

25-7108 was used in Flow cytometry/Cell sorting to evaluate the impact of sub-sterilizing pre-existing neutralizing antibody on the B-cell response to SHIV infection in rhesus macaques.

Thu Aug 17 00:00:00 EDT 2017

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 25-7108-42) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 25-7108-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Daclizumab reverses intrathecal immune cell abnormalities in multiple sclerosis.

Annals of clinical and translational neurology

Lin YC,Winokur P,Blake A,Wu T,Romm E,Bielekova B

25-7108 was used in Flow cytometry/Cell sorting to study how long-term daclizumab therapy can reverse abnormalities in immune cells critical in regulating the multiple sclerosis disease process.

Fri May 01 00:00:00 EDT 2015

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

Tue May 01 00:00:00 EDT 2007

Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

UniProt ID: (Human) P22301

Entrez Gene ID: (Human) 3586

Function(s)