|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Recombinant human Interleukin 10.|
|Storage buffer||PBS, pH 7.2, with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 ul per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Interleukin 10 (IL-10) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine.
RHCIL1001 was used in immunocytochemistry to establish a microfluidic method to analyze single cell secretions
|Konry T,Dominguez-Villar M,Baecher-Allan C,Hafler DA,Yarmush ML||Biosensors and bioelectronics (26:2707)||2011|
Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays.
RHCIL1001 was used in flow cytometry to describe a 6-color assay to analyze peripheral blood dendritic cell responses upon whole blood stimulation.
|Della Bella S,Giannelli S,Taddeo A,Presicce P,Villa ML||Journal of immunological methods (339:153)||2008|
The proportion of Th1 cells, which prevail in gut mucosa, is decreased in inflammatory bowel syndrome.
RHCIL1001 was used in flow cytometry to analyze the Th1/Th2 cytokine profile of intestinal samples from patients with inflammatory bowel disease.
|Van Damme N,De Keyser F,Demetter P,Baeten D,Mielants H,Verbruggen G,Cuvelier C,Veys EM,De Vos M||Clinical and experimental immunology (125:383)||2001|
|Not Applicable||Not Cited||
IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.
RHCIL1001 was used in ELISA to assess whether human IL-10 is produced by activated Th0, Th1-, and Th2-like CD4+ T cell clones
|Yssel H,De Waal Malefyt R,Roncarolo MG,Abrams JS,Lahesmaa R,Spits H,de Vries JE||Journal of immunology (Baltimore, Md. : 1950) (149:2378)||1992|
|Not Applicable||Not Cited||
Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones.
RHCIL1001 was used in ELISA to use lymphokines to identify subsets of human T cells
|Salgame P,Abrams JS,Clayberger C,Goldstein H,Convit J,Modlin RL,Bloom BR||Science (New York, N.Y.) (254:279)||1991|