|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Not Applicable, Rhesus monkey|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Recombinant human Interleukin 10.|
|Storage buffer||PBS, pH 7.2, with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 ul per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Interleukin 10 (IL-10) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy.
RHCIL1004 was used in flow cytometry to study the roles of the decidua-derived CCL2 in the Th2 predominance found at the maternal-fetal interface.
|He YY,He XJ,Guo PF,Du MR,Shao J,Li MQ,Li DJ||Clinical immunology (Orlando, Fla.) (145:161)||2012|
|Non-human primate||Not Cited||
In situ characterization of the granulomatous immune response with time in nonhealing lesional skin of Leishmania braziliensis-infected rhesus macaques (Macaca mulatta).
RHCIL1004 was used in flow cytometry to elucidate immune suppression mechanisms that induces granulomas in L. braziliensis-infected macaques.
|Souza-Lemos C,de-Campos SN,Teva A,Porrozzi R,Grimaldi G||Veterinary immunology and immunopathology (142:147)||2011|
|Not Applicable||Not Cited||
Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression.
RHCIL1004 was used in flow cytometry to show that human rhinoviruses alters the accessory molecule repertoire of dendritic cells
|Kirchberger S,Majdic O,Steinberger P,Blüml S,Pfistershammer K,Zlabinger G,Deszcz L,Kuechler E,Knapp W,Stöckl J||Journal of immunology (Baltimore, Md. : 1950) (175:1145)||2005|
IL-10 and IL-10 receptor overexpression in oral giant cell lesions.
RHCIL1004 was used in immunohistochemistry - frozen section to determine the expression of IL-10 and its receptor in central giant cell lesions and peripheral giant cell lesions.
|Syrio NF,Faria DR,Gomez RS,Gollob KJ,Dutra WO,Souza PE||Medicina oral, patologia oral y cirugia bucal (16:e488)||2011|
Chronic alcohol consumption is associated with an increased cytotoxic profile of circulating lymphocytes that may be related with the development of liver injury.
RHCIL1004 was used in flow cytometry to analyze the cytotoxic profile of circulating lymphocytes in chronic alcoholic patients
|Laso FJ,Almeida J,Torres E,Vaquero JM,Marcos M,Orfao A||Alcoholism, clinical and experimental research (34:876)||2010|
Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays.
RHCIL1004 was used in flow cytometry to describe a 6-color assay to analyze peripheral blood dendritic cell responses upon whole blood stimulation.
|Della Bella S,Giannelli S,Taddeo A,Presicce P,Villa ML||Journal of immunological methods (339:153)||2008|
|Rhesus monkey||Not Cited||
Phenotypic and functional characterization of long-term cultured rhesus macaque spleen-derived NKT cells.
RHCIL1004 was used in flow cytometry to describe the phenotype and function of Valpha24(+) splenic T cells from rhesus macaques.
|Gansuvd B,Hubbard WJ,Hutchings A,Thomas FT,Goodwin J,Wilson SB,Exley MA,Thomas JM||Journal of immunology (Baltimore, Md. : 1950) (171:2904)||2003|
Human NKT cells mediate antitumor cytotoxicity directly by recognizing target cell CD1d with bound ligand or indirectly by producing IL-2 to activate NK cells.
RHCIL1004 was used in flow cytometry to elucidate the mechanisms of NKT cell cytotoxicity and activation of NK cells using alpha-Galactosylceramide.
|Metelitsa LS,Naidenko OV,Kant A,Wu HW,Loza MJ,Perussia B,Kronenberg M,Seeger RC||Journal of immunology (Baltimore, Md. : 1950) (167:3114)||2001|
The proportion of Th1 cells, which prevail in gut mucosa, is decreased in inflammatory bowel syndrome.
RHCIL1004 was used in flow cytometry to analyze the Th1/Th2 cytokine profile of intestinal samples from patients with inflammatory bowel disease.
|Van Damme N,De Keyser F,Demetter P,Baeten D,Mielants H,Verbruggen G,Cuvelier C,Veys EM,De Vos M||Clinical and experimental immunology (125:383)||2001|
Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies.
RHCIL1004 was used in flow cytometry to elucidate the role of A2ARs in the regulation of immune response.
|Koshiba M,Rosin DL,Hayashi N,Linden J,Sitkovsky MV||Molecular pharmacology (55:614)||1999|
|Not Applicable||Not Cited||
IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells.
RHCIL1004 was used in ELISA to assess whether human IL-10 is produced by activated Th0, Th1-, and Th2-like CD4+ T cell clones
|Yssel H,De Waal Malefyt R,Roncarolo MG,Abrams JS,Lahesmaa R,Spits H,de Vries JE||Journal of immunology (Baltimore, Md. : 1950) (149:2378)||1992|
|Not Applicable||Not Cited||
Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones.
RHCIL1004 was used in ELISA to use lymphokines to identify subsets of human T cells
|Salgame P,Abrams JS,Clayberger C,Goldstein H,Convit J,Modlin RL,Bloom BR||Science (New York, N.Y.) (254:279)||1991|