Invitrogen

IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™

Product Image Gallery

IL-10 Antibody in Flow Cytometry (Flow) — Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.25 µg of Anti-Mouse IL-10 FITC.

IL-10 Antibody in Flow Cytometry, ELISA (Flow, ELISA) — Figure 4 Co-culture of A20 B lymphoma cells and mesenchymal stem cells (MSCs) causes a decrease in the levels of interleukin (IL)-10 in the supernatant. A20 cells (1x10 5 ) were cultured in 6-well plates in the presence or absence of MSCs at ratio 1:1 for 24, 48, or 72 h. (A) IL-10 levels in A20 culture supernatants were measured by ELISA in the presence or absence of MSCs for 72 h. (B) The fraction of CD19-positive A20 cells expressing intracellular IL-10 was measured by flow cytometry. (C) The levels of transforming growth factor (TGF)-beta, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma in the culture supernatants were measured by ELISA. (D) The fraction of cells containing intracellular IL-10 was measured by flow cytometry. Results are expressed as the means +- SD of 3 independent experiments. * P<0.05 indicates statistical significance.

IL-10 Antibody in Neutralization (Neu) — Figure 6 TGF-beta1 secreted from Tregs induces Il-17rb expression of breast cancer cells via Smad2/3/4 signaling A CD4 + CD25 + Tregs were isolated from inguinal LN of BALB/c mice injected with 4T1 cells for 3 weeks and were transwell co-cultured with 4T1 cells in the presence of control IgG or neutralizing antibodies against IL-10 or TGF-beta1. After 5-day co-culture, 4T1 cells at lower well were analyzed for Il-17rb expression by RT-qPCR analysis. Gapdh was used as internal control. B, C Western blotting analyzed Il-17rb in 4T1 cells treated with recombinant TGF-beta1 (B) or IL-10 (C) proteins for 5 days. D Western blotting analyzed Il-17rb in shLacZ or shTgfbr1 4T1 cells treated with recombinant TGF-beta1 for 5 days. E 4T1-injected BALB/c mice were sacrificed at 3 weeks postinitial injection. Total cells isolated from inguinal lymph node tissues were transwell co-cultured with 4T1 cells. After 5-day co-culture, soft-agar colony-forming activity was determined in co-cultured shLacZ or shTgfbr1 4T1 cells at lower well. (5 x 10 2 cells/well, n = 6 wells per group). F-H Western blotting analyzed Il-17rb in shLacZ, shSmad2 (F), shSmad3 (G), or shSmad4 (H) 4T1 cells treated with recombinant TGF-beta1 for 5 days. I, J WT 4T1 (I) or Il-17rb Del 4T1 (J) cells were treated with or without recombinant TGF-beta1 for 5 days. After treatment, nuclear translocation of NF-kappaB p65 was assayed by Western blotting in cells treated with recombinant mouse IL-17B for 2 h. Nuclear Matrix

Product Details

11-7101-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.5 µg/test	View 28 publications 28 publications
Neutralization (Neu)	-	View 1 publication 1 publication
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES5-16E3
Conjugate	FITC Alexa Fluor 488 Alexa Fluor 700 APC Biotin Functional Grade PE PerCP-Cyanine5.5 Unconjugated
Excitation/Emission Max	498/517 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_465403

Product Specific Information

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.

Applications Tested: This JES5-16E3 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining with flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis. test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood cells. This can be used at test size: 5 µL (0.25 µg)/test per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer.

Cancer immunology, immunotherapy : CII

Jianyi D,Haili G,Bo Y,Meiqin Y,Baoyou H,Haoran H,Fang L,Qingliang Z,Lingfei H

11-7101-82 was used in Flow Cytometry to reveal a new mechanism of BAFF-mediated mutual immune regulation between MDSCs and B cells in the occurrence and development of cervical cancer.

Sun Jan 01 00:00:00 EST 2023

RORγt phosphorylation protects against T cell-mediated inflammation.

Cell reports

Ma S,Patel SA,Abe Y,Chen N,Patel PR,Cho BS,Abbasi N,Zeng S,Schnabl B,Chang JT,Huang WJM

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2022

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Sat Nov 06 00:00:00 EDT 2021

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages.

Nature communications

Yan J,Tie G,Wang S,Tutto A,DeMarco N,Khair L,Fazzio TG,Messina LM

11-7101 was used in Flow cytometry/Cell sorting to demonstrate for the first time that type 2 diabetes mellitus induces an haematopoietic stem cell-autonomous mechanism that causes impaired wound healing.

Tue Jan 02 00:00:00 EST 2018

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Neutralization

Fri Dec 01 00:00:00 EST 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Low-Dose Intestinal Trichuris muris Infection Alters the Lung Immune Microenvironment and Can Suppress Allergic Airway Inflammation.

Infection and immunity

Chenery AL,Antignano F,Burrows K,Scheer S,Perona-Wright G,Zaph C

11-7101 was used in Flow cytometry/Cell sorting to study crosstalk between the intestine and lung during disease, showing that low-dose intestinal Trichuris muris infection alters the lung immune microenvironment.

Mon Feb 01 00:00:00 EST 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.

Cancer research

Kratochvill F,Gratz N,Qualls JE,Van De Velde LA,Chi H,Kovarik P,Murray PJ

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in ELISA

Wed Jul 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in ELISA

Wed Jul 01 00:00:00 EDT 2015

Dimethyl sulfoxide inhibits spontaneous diabetes and autoimmune recurrence in non-obese diabetic mice by inducing differentiation of regulatory T cells.

Toxicology and applied pharmacology

Lin GJ,Sytwu HK,Yu JC,Chen YW,Kuo YL,Yu CC,Chang HM,Chan DC,Huang SH

11-7101 was used in Flow cytometry/Cell sorting to demonstrate that in vivo DMSO treatment suppresses spontaneous diabetes and autoimmune recurrence in NOD mice.

Thu Jan 15 00:00:00 EST 2015

Prevention or early cure of type 1 diabetes by intranasal administration of gliadin in NOD mice.

PloS one

Funda DP,Fundova P,Hansen AK,Buschard K

11-7101 was used in Flow cytometry/Cell sorting to investigate whether intranasal (i.n.) administration of gliadin or gluten may arrest the diabetogenic process, showing that i.n. administration of gliadin prevents type 1 diabetes in NOD mice.

Tue Jan 13 00:00:00 EST 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Preemptive donor apoptotic cell infusions induce IFN-γ-producing myeloid-derived suppressor cells for cardiac allograft protection.

Journal of immunology (Baltimore, Md. : 1950)

Bryant J,Lerret NM,Wang JJ,Kang HK,Tasch J,Zhang Z,Luo X

11-7101 was used in Flow cytometry/Cell sorting to investigate the role of MDSCs in the graft protection provided by ECDI-SPs, showing that preemptive donor apoptotic cell infusions induce IFN-γ-producing MDSCs.

Sun Jun 15 00:00:00 EDT 2014

Infection rate and tissue localization of murine IL-12p40-producing monocyte-derived CD103(+) lung dendritic cells during pulmonary tuberculosis.

PloS one

Leepiyasakulchai C,Taher C,Chuquimia OD,Mazurek J,Söderberg-Naucler C,Fernández C,Sköld M

11-7101 was used in Flow cytometry/Cell sorting to demonstrate that CD103(+) dendritic cells in the lungs and pulmonary lymph nodes are monocyte-derived during pulmonary tuberculosis.

Tue Feb 11 00:00:00 EST 2014

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

PloS one

Buchwald ZS,Kiesel JR,DiPaolo R,Pagadala MS,Aurora R

11-7101 was used in Flow cytometry/Cell sorting to demonstrate the effects of activated CD8 T cell-produced cytokines on osteoclast activity.

Thu Nov 08 00:00:00 EST 2012

IL-10 regulates viral lung immunopathology during acute respiratory syncytial virus infection in mice.

PloS one

Loebbermann J,Schnoeller C,Thornton H,Durant L,Sweeney NP,Schuijs M,O'Garra A,Johansson C,Openshaw PJ

11-7101 was used in Flow cytometry/Cell sorting to study experimental respiratory syncytial virus infection in IL-10 deficient mice and mice treated with anti-IL-10 receptor antibody.

Mon Jul 23 00:00:00 EDT 2012

Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses.

Journal of immunology (Baltimore, Md. : 1950)

Walsh KP,Brady MT,Finlay CM,Boon L,Mills KH

11-7101 was used in Flow cytometry/Cell sorting to provide evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases.

Sat Aug 01 00:00:00 EDT 2009

Deletion of both ICAM-1 and C3 enhances severity of experimental autoimmune encephalomyelitis compared to C3-deficient mice.

Neuroscience letters

Smith SS,Ludwig M,Wohler JE,Bullard DC,Szalai AJ,Barnum SR

11-7101 was used in Flow cytometry/Cell sorting to examine experimental autoimmune encephalitis in ICAM-1 x C3 double knockout mice.

Fri Sep 12 00:00:00 EDT 2008

Ly49 C/I-dependent NKT cell-derived IL-10 is required for corneal graft survival and peripheral tolerance.

Journal of leukocyte biology

Watte CM,Nakamura T,Lau CH,Ortaldo JR,Stein-Streilein J

11-7101 was used in Flow cytometry/Cell sorting to test if the NK inhibitory receptor functionally contributed to tolerance development.

Tue Apr 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

Gene Aliases: CSIF; Il-10; Il10

UniProt ID: (Mouse) P18893

Entrez Gene ID: (Mouse) 16153

Function(s)

Process(es)

cytokine activity interleukin-10 receptor binding

IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™

Product Details

11-7101-82

Flow Cytometry (Flow)

Neutralization (Neu)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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