Invitrogen

IL-10 Monoclonal Antibody (JES5-16E3), Alexa Fluor™ 700, eBioscience™

Product Image Gallery

IL-10 Antibody in Flow Cytometry (Flow) — Staining of restimulated BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) followed by intracellular staining with 0.125 µg of Anti-Mouse IL-10 Alexa Fluor® 700 (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol.

IL-10 Antibody in Flow Cytometry, ELISA (Flow, ELISA) — Figure 4 Co-culture of A20 B lymphoma cells and mesenchymal stem cells (MSCs) causes a decrease in the levels of interleukin (IL)-10 in the supernatant. A20 cells (1x10 5 ) were cultured in 6-well plates in the presence or absence of MSCs at ratio 1:1 for 24, 48, or 72 h. (A) IL-10 levels in A20 culture supernatants were measured by ELISA in the presence or absence of MSCs for 72 h. (B) The fraction of CD19-positive A20 cells expressing intracellular IL-10 was measured by flow cytometry. (C) The levels of transforming growth factor (TGF)-beta, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma in the culture supernatants were measured by ELISA. (D) The fraction of cells containing intracellular IL-10 was measured by flow cytometry. Results are expressed as the means +- SD of 3 independent experiments. * P<0.05 indicates statistical significance.

IL-10 Antibody in Neutralization (Neu) — Figure 6 TGF-beta1 secreted from Tregs induces Il-17rb expression of breast cancer cells via Smad2/3/4 signaling A CD4 + CD25 + Tregs were isolated from inguinal LN of BALB/c mice injected with 4T1 cells for 3 weeks and were transwell co-cultured with 4T1 cells in the presence of control IgG or neutralizing antibodies against IL-10 or TGF-beta1. After 5-day co-culture, 4T1 cells at lower well were analyzed for Il-17rb expression by RT-qPCR analysis. Gapdh was used as internal control. B, C Western blotting analyzed Il-17rb in 4T1 cells treated with recombinant TGF-beta1 (B) or IL-10 (C) proteins for 5 days. D Western blotting analyzed Il-17rb in shLacZ or shTgfbr1 4T1 cells treated with recombinant TGF-beta1 for 5 days. E 4T1-injected BALB/c mice were sacrificed at 3 weeks postinitial injection. Total cells isolated from inguinal lymph node tissues were transwell co-cultured with 4T1 cells. After 5-day co-culture, soft-agar colony-forming activity was determined in co-cultured shLacZ or shTgfbr1 4T1 cells at lower well. (5 x 10 2 cells/well, n = 6 wells per group). F-H Western blotting analyzed Il-17rb in shLacZ, shSmad2 (F), shSmad3 (G), or shSmad4 (H) 4T1 cells treated with recombinant TGF-beta1 for 5 days. I, J WT 4T1 (I) or Il-17rb Del 4T1 (J) cells were treated with or without recombinant TGF-beta1 for 5 days. After treatment, nuclear translocation of NF-kappaB p65 was assayed by Western blotting in cells treated with recombinant mouse IL-17B for 2 h. Nuclear Matrix

Product Details

56-7101-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 17 publications 17 publications
Neutralization (Neu)	-	View 1 publication 1 publication
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 700, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES5-16E3
Conjugate	Alexa Fluor™ 700 Alexa Fluor 488 APC Biotin FITC Functional Grade PE PerCP-Cyanine5.5 Unconjugated
Excitation/Emission Max	696/719 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_891568

Product Specific Information

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: This JES5-16E3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES5-16E3 antibody has been tested by intracellular staining and flow cytometric analysis on stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

RORγt phosphorylation protects against T cell-mediated inflammation.

Cell reports

Ma S,Patel SA,Abe Y,Chen N,Patel PR,Cho BS,Abbasi N,Zeng S,Schnabl B,Chang JT,Huang WJM

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2022

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Sat Nov 06 00:00:00 EDT 2021

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Spermidine and spermine exert protective effects within the lung.

Pharmacology research & perspectives

Wawrzyniak M,Groeger D,Frei R,Ferstl R,Wawrzyniak P,Krawczyk K,Pugin B,Barcik W,Westermann P,Dreher A,Scharl M,Jutel M,Akdis CA,O Mahony L

56-7101-82 was used in Flow Cytometry to investigate the involvement of polyamine levels in asthma pathogenesis.

Sun Aug 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Neutralization

Fri Dec 01 00:00:00 EST 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.

Cancer research

Kratochvill F,Gratz N,Qualls JE,Van De Velde LA,Chi H,Kovarik P,Murray PJ

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in ELISA

Wed Jul 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in ELISA

Wed Jul 01 00:00:00 EDT 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-10 monoclonal antibody (Product # 56-7101-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

Gene Aliases: CSIF; Il-10; Il10

UniProt ID: (Mouse) P18893

Entrez Gene ID: (Mouse) 16153

Function(s)

Process(es)

cytokine activity interleukin-10 receptor binding

IL-10 Monoclonal Antibody (JES5-16E3), Alexa Fluor™ 700, eBioscience™

Product Details

56-7101-82

Flow Cytometry (Flow)

Neutralization (Neu)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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