Invitrogen

IL-10 Monoclonal Antibody (JES5-2A5), Functional Grade, eBioscience™

Product Image Gallery

IL-10 Antibody in ELISA (ELISA) — Fig. 6. Elevated IL10 expression and STAT3 activation in ADPKD kidney macrophages. (A) Elevated IL10 expression in human ADPKD kidneys. RNA was isolated from NHKs and non-cystic regions of ADPKD kidneys (three of each). IL10 expression was analyzed by performing qRT-PCR. The line indicates the mean relative value. (B) IL-10 is present in ADPKD cyst fluid. IL-10 in pooled cyst fluid collected from ADPKD kidneys from 12 different individuals was measured by using ELISA. Data are mean+-s.e.m. (C-G) Phospho-STAT3 was present in macrophages of cystic ADPKD kidneys. Formalin-fixed, paraffin-embedded tissues from ADPKD kidneys were serially sectioned (C-E), and consecutive sections were stained by using immunohistochemistry with antibodies against the M2 macrophage marker CD163 (C), phospho-STAT3 (D) or secondary antibody only (E, No Ab) as a control. Arrows indicate phospho-STAT3-positive cells in macrophage-rich regions of interstitium. (F,G) Sections of ADPKD kidneys that had been fixed and prepared in a similar manner were co-stained with anti-CD163 (green) and anti-phospho-STAT3 (red) antibodies, followed by incubation with two distinct fluorescence-labeled secondary antibodies, each with the appropriate antibody specificity (F), and with DAPI (G). Arrowheads indicate co-staining cells. n =3 biological replicates. hIL-10, human IL-10.

IL-10 Antibody in Neutralization (Neu) — Published figure using IL-10 monoclonal antibody (Product # 16-7102-81) in Neutralization

Product Details

16-7102-81

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
ELISA (ELISA)	-	View 6 publications 6 publications
Neutralization (Neu)	Assay-Dependent	View 8 publications 8 publications
Functional Assay (FN)	Assay-Dependent	View 5 publications 5 publications
Inhibition Assays (IA)	-	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES5-2A5
Conjugate	Functional Grade Biotin
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_469226

Product Specific Information

Description: The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody is a neutralizing antibody. Mouse IL-10 is an 18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: The JES5-2A5 antibody has been reported for use in ELISA, ELISPOT, neutralization of IL-10 bioactivity, and intracellular staining for flow cytometric analysis.

Applications Tested: The Functional Grade Purified JES5-2A5 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-10 bioactivity.

The JES5-2A5 antibody at 0.004 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-10 in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

The biotinylated JES5-2A5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-10 (IL-10) in combination with the purified JES5-16E3 (14-7101) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CNS-targeted autoimmunity leads to increased influenza mortality in mice.

The Journal of experimental medicine

Glenn JD,Smith MD,Xue P,Chan-Li Y,Collins S,Calabresi PA,Horton MR,Whartenby KA

16-7102 was used in Inhibition experiments to investigate the mechanisms responsible for the enhanced susceptibility to influenza mortality resulting from central nervous system-targeted autoimmunity.

Wed Feb 01 00:00:00 EST 2017

Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Howes A,Taubert C,Blankley S,Spink N,Wu X,Graham CM,Zhao J,Saraiva M,Ricciardi-Castagnoli P,Bancroft GJ,O'Garra A

16-7102 was used in an ELISA assay to highlight key pathways responsible for the regulation of pro- and anti-inflammatory cytokines in macrophages and reveal how they may differ according to the genetic background of the host.

Sat Oct 01 00:00:00 EDT 2016

Autocrine IL-10 activation of the STAT3 pathway is required for pathological macrophage differentiation in polycystic kidney disease.

Disease models & mechanisms

Peda JD,Salah SM,Wallace DP,Fields PE,Grantham CJ,Fields TA,Swenson-Fields KI

16-7102 was used in Neutralization experiments to functionally characterise M2-like macrophages in polycycstic kidney disease.

Thu Sep 01 00:00:00 EDT 2016

Autocrine IL-10 activation of the STAT3 pathway is required for pathological macrophage differentiation in polycystic kidney disease.

Disease models & mechanisms

Peda JD,Salah SM,Wallace DP,Fields PE,Grantham CJ,Fields TA,Swenson-Fields KI

16-7102 was used in Neutralization experiments to functionally characterise M2-like macrophages in polycycstic kidney disease.

Thu Sep 01 00:00:00 EDT 2016

Neuroantigen-specific autoregulatory CD8+ T cells inhibit autoimmune demyelination through modulation of dendritic cell function.

PloS one

Kashi VP,Ortega SB,Karandikar NJ

16-7102 was used in Inhibition experiments to demonstrate a complex interplay between CNS-specific CD8+ T cells, DC and pathogenic CD4+ T cells.

Tue Nov 10 00:00:00 EST 2015

Tr1 Cells, but Not Foxp3+ Regulatory T Cells, Suppress NLRP3 Inflammasome Activation via an IL-10-Dependent Mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Yao Y,Vent-Schmidt J,McGeough MD,Wong M,Hoffman HM,Steiner TS,Levings MK

16-7102 was used in Blocking experiments to compare the ability of Tr1 cells versus Foxp3(+) Tregs to suppress IL-1β production from macrophages following LPS and ATP stimulation.

Wed Jul 15 00:00:00 EDT 2015

The mucosal inflammatory response to non-typhoidal Salmonella in the intestine is blunted by IL-10 during concurrent malaria parasite infection.

Mucosal immunology

Mooney JP,Butler BP,Lokken KL,Xavier MN,Chau JY,Schaltenberg N,Dandekar S,George MD,Santos RL,Luckhart S,Tsolis RM

16-7102 was used in Functional assays to study the effect of concurrent Salmonella and Malaria infection on the intestinal mucosal inflammatory response.

Sat Nov 01 00:00:00 EDT 2014

Differential gene expression in thrombomodulin (TM; CD141)(+) and TM(-) dendritic cell subsets.

PloS one

Toda M,Shao Z,Yamaguchi KD,Takagi T,D'Alessandro-Gabazza CN,Taguchi O,Salamon H,Leung LL,Gabazza EC,Morser J

16-7102 was used in Flow cytometry/Cell sorting to show that thrombomodulin treatment increases the number of thrombomodulin(+) dendritic cells, which have different gene expression to thrombomodulin(-) dendritic cells in immune pathways.

Wed Apr 16 00:00:00 EDT 2014

The sst1 resistance locus regulates evasion of type I interferon signaling by Chlamydia pneumoniae as a disease tolerance mechanism.

PLoS pathogens

He X,Berland R,Mekasha S,Christensen TG,Alroy J,Kramnik I,Ingalls RR

Mon Mar 17 00:00:00 EDT 2014

Development of severe pathology in immunized pregnant mice challenged with lethal malaria parasites.

Infection and immunity

Mineo S,Niikura M,Inoue S,Kuroda M,Kobayashi F

16-7102 was used in an ELISA assay to investigate the pathogenesis of maternal malaria.

Tue Oct 01 00:00:00 EDT 2013

Schistosoma japonicum soluble egg antigens attenuate IFN-γ-induced MHC class II expression in RAW 264.7 macrophages.

PloS one

Tang GX,Zhou HJ,Xu JW,Xu JM,Ji MJ,Wu HW,Wu GL

16-7102 was used in Blocking experiments to elucidate the innate immune-related molecular events involved in the interaction between S. japonicum and its host.

Tue May 07 00:00:00 EDT 2013

Schistosoma japonicum soluble egg antigens attenuate IFN-γ-induced MHC class II expression in RAW 264.7 macrophages.

PloS one

Tang GX,Zhou HJ,Xu JW,Xu JM,Ji MJ,Wu HW,Wu GL

16-7102 was used in Blocking experiments to elucidate the innate immune-related molecular events involved in the interaction between S. japonicum and its host.

Tue May 07 00:00:00 EDT 2013

Modified Foxp3 mRNA protects against asthma through an IL-10-dependent mechanism.

The Journal of clinical investigation

Mays LE,Ammon-Treiber S,Mothes B,Alkhaled M,Rottenberger J,Müller-Hermelink ES,Grimm M,Mezger M,Beer-Hammer S,von Stebut E,Rieber N,Nürnberg B,Schwab M,Handgretinger R,Idzko M,Hartl D,Kormann MS

16-7102 was used in Flow cytometry/Cell sorting to show that modified Foxp3 mRNA rebalanced pulmonary T helper cell responses and protected from allergen-induced tissue inflammation, airway hyperresponsiveness, and goblet cell metaplasia in mice.

Fri Mar 01 00:00:00 EST 2013

Role of TLR2-dependent IL-10 production in the inhibition of the initial IFN-γ T cell response to Porphyromonas gingivalis.

Journal of leukocyte biology

Gaddis DE,Maynard CL,Weaver CT,Michalek SM,Katz J

16-7102 was used in Flow cytometry/Cell sorting to highlight the role of IL-10 in inhibiting T cell responses to the initial systemic P.g. exposure.

Tue Jan 01 00:00:00 EST 2013

CD28 ligation increases macrophage suppression of T-cell proliferation.

Cellular & molecular immunology

Silberman D,Bucknum A,Bartlett T,Composto G,Kozlowski M,Walker A,Werda A,Cua J,Sharpe AH,Somerville JE,Riggs JE

16-7102 was used in Functional assays to investigate the ability of exogenous T-cell costimulation to recover the peritoneal T-cell response.

Sun Jul 01 00:00:00 EDT 2012

Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantation.

The Journal of experimental medicine

Hashimoto D,Chow A,Greter M,Saenger Y,Kwan WH,Leboeuf M,Ginhoux F,Ochando JC,Kunisaki Y,van Rooijen N,Liu C,Teshima T,Heeger PS,Stanley ER,Frenette PS,Merad M

16-7102 was used in Neutralization experiments to investigate the contribution of host macrophages in graft-versus-host disease.

Mon May 09 00:00:00 EDT 2011

Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma.

Nature immunology

Lajoie S,Lewkowich IP,Suzuki Y,Clark JR,Sproles AA,Dienger K,Budelsky AL,Wills-Karp M

16-7102 was used in Western Blotting to demonstrate that IL-17A mediated severe airway hyperresponsiveness in susceptible strains of mice by enhancing IL-13-driven responses.

Fri Oct 01 00:00:00 EDT 2010

Distinct populations of metastases-enabling myeloid cells expand in the liver of mice harboring invasive and preinvasive intra-abdominal tumor.

Journal of leukocyte biology

Connolly MK,Mallen-St Clair J,Bedrosian AS,Malhotra A,Vera V,Ibrahim J,Henning J,Pachter HL,Bar-Sagi D,Frey AB,Miller G

16-7102 was used in Flow cytometry/Cell sorting to show that liver myeloid-derived suppressor cells accelerate the development of hepatic metastases by inactivation of cytotoxic T cells.

Thu Apr 01 00:00:00 EDT 2010

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE

16-7102 was used in an ELISA assay to study the importance of the chemokine induction activity of host defence peptides in protection against bacterial infections.

Mon Mar 01 00:00:00 EST 2010

Development of murine lupus involves the combined genetic contribution of the SLAM and FcgammaR intervals within the Nba2 autoimmune susceptibility locus.

Journal of immunology (Baltimore, Md. : 1950)

Jørgensen TN,Alfaro J,Enriquez HL,Jiang C,Loo WM,Atencio S,Bupp MR,Mailloux CM,Metzger T,Flannery S,Rozzo SJ,Kotzin BL,Rosemblatt M,Bono MR,Erickson LD

16-7102 was used in an ELISpot assay to identify gene intervals within Nba2 that control the development of autoantibody-producing B cells.

Fri Jan 15 00:00:00 EST 2010

Regulatory T cells induced by GM-CSF suppress ongoing experimental myasthenia gravis.

Clinical immunology (Orlando, Fla.)

Sheng JR,Li LC,Ganesh BB,Prabhakar BS,Meriggioli MN

16-7102 was used in Flow cytometry/Cell sorting to show that EAMG induced by repeated immunizations with acetylcholine receptor protein in C57BL6 mice is effectively suppressed by GM-CSF treatment.

Fri Aug 01 00:00:00 EDT 2008

Galectin-9 protects mice from the Shwartzman reaction by attracting prostaglandin E2-producing polymorphonuclear leukocytes.

Clinical immunology (Orlando, Fla.)

Tsuboi Y,Abe H,Nakagawa R,Oomizu S,Watanabe K,Nishi N,Nakamura T,Yamauchi A,Hirashima M

Wed Aug 01 00:00:00 EDT 2007

Infection of dendritic cells by a gamma2-herpesvirus induces functional modulation.

Journal of immunology (Baltimore, Md. : 1950)

Flaño E,Kayhan B,Woodland DL,Blackman MA

16-7102 was used in Immuno-assay to indicate that gamma2-herpesvirus infection of DCs is a mechanism of viral immune evasion, partially mediated by IL-10.

Thu Sep 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

Gene Aliases: CSIF; Il-10; Il10

UniProt ID: (Mouse) P18893

Entrez Gene ID: (Mouse) 16153

Function(s)