|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Pig, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Recombinant mouse IL-12|
|Purification||Caprylic acid precipitation|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM120 targets IL-12 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM120 immunogen is recombinant mouse IL-12.
MM120 detects IL-12 which has a predicted molecular weight of approximately 22 kDa.
The MM120 mouse IL12 antibody (clone 9A5) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM121B (biotinylated conjugate of clone C17-8). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM120 (clone 9A5) and biotinylated antibody MM121B (clone C17-8) have successfully been used in combination with recombinant mouse IL12p70 protein SMIL12 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes.
MM120 was used in ELISA to investigate the role of listeriolysin O in the calpain activation and interleukin-1 alpha secretion in Listeria monocytogene-infected macrophages
|Dewamitta SR,Nomura T,Kawamura I,Hara H,Tsuchiya K,Kurenuma T,Shen Y,Daim S,Yamamoto T,Qu H,Sakai S,Xu Y,Mitsuyama M||Infection and immunity (78:1884)||2010|
Involvement of IL-10 in exhaustion of myeloid dendritic cells and rescue by CD40 stimulation.
MM120 was used in ELISA to study the role of interleukin 10 in the function of myeloid dendritic cells
|Kajino K,Nakamura I,Bamba H,Sawai T,Ogasawara K||Immunology (120:28)||2007|
|Not Applicable||Not Cited||
Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.
MM120 was used in ELISA to describe the mechanisms by which tumor-infiltrating macrophages and dendritic cells can be redirected to become potent effectors and activators of the innate and adaptive immunity
|Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP||Cancer research (65:3437)||2005|
Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni.
MM120 was used in ELISA to investigate the promoting effect of interleukin 12 and schistosome egg antigen on substance P receptor expression
|Blum AM,Metwali A,Crawford C,Li J,Qadir K,Elliott DE,Weinstock JV||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (15:950)||2001|
Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.
MM120 was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses
|Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH||Journal of immunology (Baltimore, Md. : 1950) (161:4146)||1998|
Characterization of early IL-12, IFN-alphabeta, and TNF effects on antiviral state and NK cell responses during murine cytomegalovirus infection.
MM120 was used in ELISA to investigate the importance of tumor necrosis factor, interleukin 12 and interferon alpha beta in immune responses against cytomegalovirus infection
|Orange JS,Biron CA||Journal of immunology (Baltimore, Md. : 1950) (156:4746)||1996|
Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.
MM120 was used in ELISA to investigate the role of interleukin 12 in Th1 cell function
|Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G||European journal of immunology (26:659)||1996|
|Not Applicable||Not Cited||
Protection of non-obese diabetic mice from autoimmune diabetes by Escherichia coli heat-labile enterotoxin B subunit.
MM120 was used in immunohistochemistry to show that EtxB can block diabetes
|Ola TO,Williams NA||Immunology (117:262)||2006|
Hepatic Na(+)-K(+)-ATPase enzyme activity correlates with polarized beta-subunit expression.
MM120 was used in immunohistochemistry to investigate the relationship between hepatic sodium/potassium ATPase activity and the expression of polarized beta subunit
|Simon FR,Leffert HL,Ellisman M,Iwahashi M,Deerinck T,Fortune J,Morales D,Dahl R,Sutherland E||The American journal of physiology (269:C69)||1995|
Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.
MM120 was used in blocking/activating experiment to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells
|DeKruyff RH,Fang Y,Umetsu DT||Journal of immunology (Baltimore, Md. : 1950) (160:2231)||1998|
Use of a monoclonal antibody to quantify (Na+,K+)-ATPase activity and sites in normal and regenerating rat liver.
MM120 was used in blocking/activating experiment to investigate the activity and sites of sodium/potassium ATPase in normal and regenerating rat liver
|Schenk DB,Hubert JJ,Leffert HL||The Journal of biological chemistry (259:14941)||1984|
Identification of monoclonal antibody binding domains of Na+,K(+)-ATPase by immunoelectron microscopy.
MM120 was used in immunoprecipitation to investigate the binding domains of sodium/potassium ATPase antibody
|Ting-Beall HP,Beall HC,Hastings DF,Friedman ML,Ball WJ||FEBS letters (265:121)||1990|
Rat hepatic (Na+, K+)-ATPase: alpha-subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping.
MM120 was used in immunoprecipitation to investigate the structure of sodium/potassium ATPase in rat liver
|Hubert JJ,Schenk DB,Skelly H,Leffert HL||Biochemistry (25:4156)||1986|