|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-12(p70) (CHO cell-derived)|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM121 targets IL-12 in ELISA and WB applications and shows reactivity with mouse samples.
The MM121 immunogen is recombinant mouse IL-12(p70) (CHO cell-derived).
MM121 detects IL-12 which has a predicted molecular weight of approximately 22 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A polymorphism in the coding region of Il12b promotes IL-12p70 and IL-23 heterodimer formation.
MM121 was used in western blot to investigate the effect of a polymorphism in the coding region of Il12b for interleukin-12p70 and interleukin-23 heterodimer formation
|Zwiers A,Fuss IJ,Seegers D,Konijn T,Garcia-Vallejo JJ,Samsom JN,Strober W,Kraal G,Bouma G||Journal of immunology (Baltimore, Md. : 1950) (186:3572)||2011|
|Not Applicable||0.5 µg/ml||
Ethanol consumption modifies dendritic cell antigen presentation in mice.
MM121 was used in ELISA to assess the influence of alcohol consumption on antigen-presenting cell populations and cytokine production in type 1 cell-mediated adaptive immune responses
|Heinz R,Waltenbaugh C||Alcoholism, clinical and experimental research (31:1759)||2007|
Heligmosomoides polygyrus promotes regulatory T-cell cytokine production in the murine normal distal intestine.
MM121 was used in ELISA to investigate the effect of exposure to Heligmosomoides polygyrus on cytokine pathways in murine normal distal intestine
|Setiawan T,Metwali A,Blum AM,Ince MN,Urban JF,Elliott DE,Weinstock JV||Infection and immunity (75:4655)||2007|
The influence of collagenase treatment on the production of TNF-alpha, IL-6 and IL-10 by testicular macrophages.
MM121 was used in ELISA to study the effect of collagenase treatment on cytokine production by testicular macrophages
|Bryniarski K,Szczepanik M,Ptak M,Ptak W||Journal of immunological methods (301:186)||2005|
Modulation of testicular macrophage activity by collagenase.
MM121 was used in ELISA to investigate the regulation of cytokines and the expression of ROI in testicular macrophages
|Bryniarski K,Szczepanik M,Ptak M,Ptak W||Folia histochemica et cytobiologica (43:37)||2005|
|Not Applicable||Not Cited||
Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.
MM121 was used in ELISA to describe the mechanisms by which tumor-infiltrating macrophages and dendritic cells can be redirected to become potent effectors and activators of the innate and adaptive immunity
|Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP||Cancer research (65:3437)||2005|
Heligmosomoides polygyrus inhibits established colitis in IL-10-deficient mice.
MM121 was used in ELISA to investigate the effect of Heligmosomoides polygyrus on colitis
|Elliott DE,Setiawan T,Metwali A,Blum A,Urban JF,Weinstock JV||European journal of immunology (34:2690)||2004|
Modulation of macrophage activity by proteolytic enzymes. Differential regulation of IL-6 and reactive oxygen intermediates (ROIs) synthesis as a possible homeostatic mechanism in the control of inflammation.
MM121 was used in ELISA to investigate the effect of collagenase and trypsin for the regulation of macrophage function
|Bryniarski K,Maresz K,Szczepanik M,Ptak M,Ptak W||Inflammation (27:333)||2003|
NF-kappa B is required within the innate immune system to inhibit microflora-induced colitis and expression of IL-12 p40.
MM121 was used in ELISA to study the role of NF-kappa B and the innate immune system in inhibiting microflora-induced colitis
|Tomczak MF,Erdman SE,Poutahidis T,Rogers AB,Holcombe H,Plank B,Fox JG,Horwitz BH||Journal of immunology (Baltimore, Md. : 1950) (171:1484)||2003|
Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.
MM121 was used in ELISA to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells
|DeKruyff RH,Fang Y,Umetsu DT||Journal of immunology (Baltimore, Md. : 1950) (160:2231)||1998|
In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice.
MM121 was used in blocking or activating experiment to characterize specific memory T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice
|Kirby AC,Sundquist M,Wick MJ||Infection and immunity (72:6390)||2004|
Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination.
MM121 was used in blocking/activating experiment to investigate the effect of dendritic cell vaccination for the prevention of tumor migration
|Kim A,Noh YW,Kim KD,Jang YS,Choe YK,Lim JS||Experimental and molecular medicine (36:428)||2004|
CD40 ligation prevents neonatal induction of transplantation tolerance.
MM121 was used in blocking/activating experiment to study the effect of CD40 ligation on neonatal transplantation tolerance
|Flamand V,Donckier V,Demoor FX,Le Moine A,Matthys P,Vanderhaeghen ML,Tagawa Y,Iwakura Y,Billiau A,Abramowicz D,Goldman M||Journal of immunology (Baltimore, Md. : 1950) (160:4666)||1998|
Characterization of early IL-12, IFN-alphabeta, and TNF effects on antiviral state and NK cell responses during murine cytomegalovirus infection.
MM121 was used in blocking/activating experiment to investigate the importance of tumor necrosis factor, interleukin 12 and interferon alpha beta in immune responses against cytomegalovirus infection
|Orange JS,Biron CA||Journal of immunology (Baltimore, Md. : 1950) (156:4746)||1996|
Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.
MM121 was used in blocking/activating experiment to investigate the role of interleukin 12 in Th1 cell function
|Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G||European journal of immunology (26:659)||1996|
Quantities of interleukin-12p40 in mature CD8alpha negative dendritic cells correlate with strength of TCR signal and determine Th cell development.
MM121 was used in ELISA and flow cytometry to report that the strength of TCR signaling directs Th cell development by regulating CD40L expression on T cells which determines production of IL-12p40 from DC via CD40.
|Fujimoto N,Ishida H,Nakamura I,Ogasawara K,Itoh Y||Microbiology and immunology (47:1017)||2003|