|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant human IL-12 (p70)|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
M121 targets IL-12 in ELISA, IA, and WB applications and shows reactivity with Human samples.
The M121 immunogen is recombinant human IL-12 (p70).
M121 detects IL-12 which has a predicted molecular weight of approximately 23 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product is a Low Endotoxin formulation.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis C virus modulates human monocyte-derived dendritic cells.
M121 was used in ELISA to study the effect of hepatitis C virus on human monocyte-derived dendritic cells
|Eksioglu EA,Bess JR,Zhu H,Xu Y,Dong HJ,Elyar J,Nelson DR,Liu C||Journal of viral hepatitis (17:757)||2010|
|Not Applicable||Not Cited||
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
M121 was used in ELISA to compare the effect of different immunosuppressive regimens on cytokine expression in the grafted lung
|Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M||Transplantation proceedings (35:1523)||2003|
Monocyte induction of IL-10 and down-regulation of IL-12 by iC3b deposited in ultraviolet-exposed human skin.
M121 was used in ELISA to investigate the regulation of interleukin-10 and interleukin-12 by iC3b deposited in ultraviolet-exposed human skin
|Yoshida Y,Kang K,Berger M,Chen G,Gilliam AC,Moser A,Wu L,Hammerberg C,Cooper KD||Journal of immunology (Baltimore, Md. : 1950) (161:5873)||1998|
IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells.
M121 was used in ELISA to investigate the importance of PGE2 during dendritic cell development
|Kali¿ski P,Hilkens CM,Snijders A,Snijdewint FG,Kapsenberg ML||Journal of immunology (Baltimore, Md. : 1950) (159:28)||1997|
Reduced IL-12 production by monocytes upon ultraviolet-B irradiation selectively limits activation of T helper-1 cells.
M121 was used in ELISA to investigate the influence of UVB irradiation on the activation of human T helper cells
|Kremer IB,Hilkens CM,Sylva-Steenland RM,Koomen CW,Kapsenberg ML,Bos JD,Teunissen MB||Journal of immunology (Baltimore, Md. : 1950) (157:1913)||1996|
Regulation of bioactive IL-12 production in lipopolysaccharide-stimulated human monocytes is determined by the expression of the p35 subunit.
M121 was used in ELISA to investigate the regulation of interleukin 12 expression
|Snijders A,Hilkens CM,van der Pouw Kraan TC,Engel M,Aarden LA,Kapsenberg ML||Journal of immunology (Baltimore, Md. : 1950) (156:1207)||1996|
Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
M121 was used in ELISA to investigate the role of interleukin 12 on immune responses and Th cell development
|Manetti R,Parronchi P,Giudizi MG,Piccinni MP,Maggi E,Trinchieri G,Romagnani S||The Journal of experimental medicine (177:1199)||1993|
Lyme arthritis synovial gammadelta T cells instruct dendritic cells via fas ligand.
M121 was used in blocking/activating experiment to investigate the mechanism of dendritic cell activation by lyme arthritis synovial gamma delta T cells
|Collins C,Wolfe J,Roessner K,Shi C,Sigal LH,Budd RC||Journal of immunology (Baltimore, Md. : 1950) (175:5656)||2005|
Stimulation of human T lymphocytes by LPS is MHC unrestricted, but strongly dependent on B7 interactions.
M121 was used in blocking/activating experiment to study the role of CD28 and CTLA-4 in activation of human T cells by LPS
|Mattern T,Flad HD,Brade L,Rietschel ET,Ulmer AJ||Journal of immunology (Baltimore, Md. : 1950) (160:3412)||1998|