Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Applications Reported: The C8.6 antibody has been reported for use in ELISA.
Applications Tested: The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the purified B-T21 (13-7128) antibody for capture and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5 - 2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p40 monomer in combination with the purified C8.3 (14-7127) antibody for capture and recombinant human IL-12 p40 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10000 pg/mL - 80 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 p40; IL-12 subunit p40; IL-12B; Il-12p40; IL-23 subunit alpha; IL-23 subunit p40; IL-23-A; IL-23p19; IL12, subunit p40; IL12B; IL12P40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-12 beta chain; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF2; RP23-388G23.1
Gene Aliases: CLMF; CLMF2; IL-12B; IL-23; IL-23A; IL12B; IL23A; IL23P19; IMD28; IMD29; NKSF; NKSF2; P19; SGRF; UNQ2498/PRO5798