Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Applications Reported: Unlabeled C8.6 has been reported for use as a cold mAb control to block fluorochrome conjugated C8.6 in staining of intracellular IL-12 and in immunblotting under nonreducing conditions. C8.6 has also been reported for use in cytokine neutralization. (Please use Functional Grade purified C8.6, cat. 16-7129, in functional assays.).
Applications Tested: The unconjugated C8.6 antibody has been tested as a cold mAb control to block fluorochrome conjugated C8.6 in staining of intracellular IL-12 and nonreducing immunoblots at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 p40; IL-12 subunit p40; IL-12B; Il-12p40; IL-23 subunit p40; IL12, subunit p40; IL12B; IL12P40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; Interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF2; RP23-388G23.1
Gene Aliases: CLMF; CLMF2; IL-12B; IL12B; IMD28; IMD29; NKSF; NKSF2
UniProt ID: (Human) P29460
Entrez Gene ID: (Human) 3593