Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C8.6)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Details

M121

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 7 publications 7 publications

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

C8.6

Immunogen

COS cell-derived recombinant human IL-12, p40 subunit

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

RRID

AB_223554

Product Specific Information

This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.

This is a low-endotoxin formulation.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hepatitis C virus modulates human monocyte-derived dendritic cells.

Journal of viral hepatitis

Eksioglu EA,Bess JR,Zhu H,Xu Y,Dong HJ,Elyar J,Nelson DR,Liu C

M121 was used in ELISA to study the effect of hepatitis C virus on human monocyte-derived dendritic cells

Mon Nov 01 00:00:00 EDT 2010

Lyme arthritis synovial gammadelta T cells instruct dendritic cells via fas ligand.

Journal of immunology (Baltimore, Md. : 1950)

Collins C,Wolfe J,Roessner K,Shi C,Sigal LH,Budd RC

M121 was used in blocking/activating experiment to investigate the mechanism of dendritic cell activation by lyme arthritis synovial gamma delta T cells

Tue Nov 01 00:00:00 EST 2005

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M121 was used in ELISA to compare the effect of different immunosuppressive regimens on cytokine expression in the grafted lung

Sun Jun 01 00:00:00 EDT 2003

Monocyte induction of IL-10 and down-regulation of IL-12 by iC3b deposited in ultraviolet-exposed human skin.

Journal of immunology (Baltimore, Md. : 1950)

Yoshida Y,Kang K,Berger M,Chen G,Gilliam AC,Moser A,Wu L,Hammerberg C,Cooper KD

M121 was used in ELISA to investigate the regulation of interleukin-10 and interleukin-12 by iC3b deposited in ultraviolet-exposed human skin

Tue Dec 01 00:00:00 EST 1998

Stimulation of human T lymphocytes by LPS is MHC unrestricted, but strongly dependent on B7 interactions.

Journal of immunology (Baltimore, Md. : 1950)

Mattern T,Flad HD,Brade L,Rietschel ET,Ulmer AJ

M121 was used in blocking/activating experiment to study the role of CD28 and CTLA-4 in activation of human T cells by LPS

Wed Apr 01 00:00:00 EST 1998

IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells.

Journal of immunology (Baltimore, Md. : 1950)

Kaliński P,Hilkens CM,Snijders A,Snijdewint FG,Kapsenberg ML

M121 was used in ELISA to investigate the importance of PGE2 during dendritic cell development

Tue Jul 01 00:00:00 EDT 1997

Reduced IL-12 production by monocytes upon ultraviolet-B irradiation selectively limits activation of T helper-1 cells.

Journal of immunology (Baltimore, Md. : 1950)

Kremer IB,Hilkens CM,Sylva-Steenland RM,Koomen CW,Kapsenberg ML,Bos JD,Teunissen MB

M121 was used in ELISA to investigate the influence of UVB irradiation on the activation of human T helper cells

Sun Sep 01 00:00:00 EDT 1996

Regulation of bioactive IL-12 production in lipopolysaccharide-stimulated human monocytes is determined by the expression of the p35 subunit.

Journal of immunology (Baltimore, Md. : 1950)

Snijders A,Hilkens CM,van der Pouw Kraan TC,Engel M,Aarden LA,Kapsenberg ML

M121 was used in ELISA to investigate the regulation of interleukin 12 expression

Thu Feb 01 00:00:00 EST 1996

Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.

The Journal of experimental medicine

Manetti R,Parronchi P,Giudizi MG,Piccinni MP,Maggi E,Trinchieri G,Romagnani S

M121 was used in ELISA to investigate the role of interleukin 12 on immune responses and Th cell development

Thu Apr 01 00:00:00 EST 1993

Bioinformatics

Protein Aliases: CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p40; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; IL12, subunit p40; il23; IL23 p41; ILN; Interleukin; Interleukin 12; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-12 beta chain; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; Interleukin12; JKA3 induced upon T-cell activation; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF2; RP23-388G23.1

Gene Aliases: CLMF; CLMF2; IL-12B; IL-23; IL-23A; IL12B; IL23A; IL23P19; IMD28; IMD29; NKSF; NKSF2; P19; SGRF; UNQ2498/PRO5798

UniProt ID: (Human) P29460, (Human) Q9NPF7

Entrez Gene ID: (Human) 3593, (Human) 51561

Molecular Function: transmembrane signal receptor cytokine immunoglobulin interleukin superfamily intercellular signal molecule