|Flow Cytometry (Flow)||5 µL (0.06 µg)/test|
|Host / Isotype||Mouse / IgG1, kappa|
|Recommended Isotype Control||Mouse IgG1 kappa Isotype Control, PE, eBioscience™|
|Clone||eBioHP40 (HP40, HP-40)|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, store in dark, DO NOT FREEZE!|
Description: The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope.
Applications Reported: The C8.6 antibody has been reported for use in intracellular flow cytometric analysis.
Applications Tested: This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
IL-23 is a member of the IL-12 cytokine family which is produced by dendritic cells and other antigen presenting cells in the peripheral tissues. This cytokine is a heterodimer composed of an IL-12p40 subunit, and a p19 subunit. IL-23 plays an important role in stimulating memory T-cells. It is also required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells. Th17 cells play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLMF p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 p40; IL-12 subunit p40; IL-12B; Il-12p40; IL-23 subunit p40; IL12, subunit p40; IL12B; IL12P40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; Interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF2; RP23-388G23.1
Gene Aliases: CLMF; CLMF2; IL-12B; IL12B; IMD28; IMD29; NKSF; NKSF2
UniProt ID: (Human) P29460
Entrez Gene ID: (Human) 3593
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