Intracellular staining of unstimulated (left) or IFN gamma (Product # 14-8139), LPS, and monensin stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149) and Anti-Human IL-12/IL-23 p40 PE. Cells in the monocyte gate were used for analysis.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Clone||eBioHP40 (HP40, HP-40)|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 µL (0.06 µg)/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope.
Applications Reported: The C8.6 antibody has been reported for use in intracellular flow cytometric analysis.
Applications Tested: This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
IL-23 is a member of the IL-12 cytokine family which is produced by dendritic cells and other antigen presenting cells in the peripheral tissues. This cytokine is a heterodimer composed of an IL-12p40 subunit, and a p19 subunit. IL-23 plays an important role in stimulating memory T-cells. It is also required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells. Th17 cells play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.