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|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human IL-12, p40 expressed in CHO cells.|
|Storage buffer||PBS, pH 7.2|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the p40 subunit and the p70 heterodimer of human IL-12, and can be used as a capture antibody in ELISA assays. It does not effectively neutralize IL-12.
AHC9122 is >98% pure by SDS-PAGE. Further dilutions should be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA. The addition of a preservative is also recommended for long term storage. For maximal stability, apportion into working aliquots and store at -70°C, avoiding repeated freeze-thaw cycles.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
AHC9122 was used in flow cytometry to characterize human plasmacytoid dendritic cells from breast cancer patients
|Cox K,North M,Burke M,Singhal H,Renton S,Aqel N,Islam S,Knight SC||Journal of leukocyte biology (78:1142)||2005|
|Non-human primate||Not Cited||
Measles virus infection of rhesus macaques affects neutrophil expression of IL-12 and IL-10.
AHC9122 was used in flow cytometry and immunocytochemistry to examine IL-12 and IL-10 production by neutrophils isolated from naive or measles virus-infected rhesus macaques.
|Hoffman SJ,Polack FP,Hauer DA,Griffin DE||Viral immunology (16:369)||2003|
|Non-human primate||Not Cited||
Altered synthesis of interleukin-12 and type 1 and type 2 cytokinesin rhesus macaques during measles and atypical measles.
AHC9122 was used in flow cytometry to investigate the role of IL-12 in measles induced immunosuppression.
|Polack FP,Hoffman SJ,Moss WJ,Griffin DE||The Journal of infectious diseases (185:13)||2002|
CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL12p35; IL12P40; IL35 subunit; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2
CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35