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      View (16) other IL-12 p70 antibodies

      Invitrogen

      IL-12 p70 Monoclonal Antibody (C11.5)

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      Catalog #:AHC9122

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      Product Details

      TESTED APPLICATIONS DILUTION
      ELISA (ELISA) 1-10 µg/mL
      PUBLISHED APPLICATIONS
      Flow Cytometry (Flow) See 1 publications below
      Miscellaneous PubMed (MISC) See 2 publications below
      Tested Species reactivity Human
      Published species reactivity Non-human primate
      Host / Isotype Mouse / IgG1
      Class Monoclonal
      Type Antibody
      Clone C11.5
      Immunogen Recombinant human IL-12, p40 expressed in CHO cells.
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 µg/ml
      Purification Protein A/G
      Storage buffer PBS, pH 7.2
      Contains no preservative
      Storage conditions -80°C
      RRID AB_2536295

      Product Specific Information

      This antibody recognizes the p40 subunit and the p70 heterodimer of human IL-12, and can be used as a capture antibody in ELISA assays. It does not effectively neutralize IL-12.

      AHC9122 is >98% pure by SDS-PAGE. Further dilutions should be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA. The addition of a preservative is also recommended for long term storage. For maximal stability, apportion into working aliquots and store at -70°C, avoiding repeated freeze-thaw cycles.

      Background/Target Information

      This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (3)

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      Bioinformatics

      Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL12p35; IL12P40; IL35 subunit; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2

      Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35

      UniProt ID: (Human) P29459, (Human) P29460

      Entrez Gene ID: (Human) 3592, (Human) 3593

      Molecular Function: cytokine interleukin superfamily signaling molecule

      Function(s) Blue Arrow Icon
      positive regulation of T cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target immune response cell cycle arrest response to virus response to UV-B cell migration response to lipopolysaccharide negative regulation of interleukin-17 production positive regulation of interferon-gamma production positive regulation of natural killer cell activation positive regulation of mononuclear cell proliferation positive regulation of smooth muscle cell apoptotic process positive regulation of T cell proliferation positive regulation of tyrosine phosphorylation of Stat4 protein defense response to protozoan positive regulation of T cell differentiation positive regulation of cell adhesion positive regulation of natural killer cell mediated cytotoxicity negative regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation defense response to Gram-positive bacterium positive regulation of NK T cell activation cellular response to lipopolysaccharide extrinsic apoptotic signaling pathway cellular response to virus positive regulation of dendritic cell chemotaxis positive regulation of defense response to virus by host natural killer cell activation involved in immune response positive regulation of T-helper 1 type immune response negative regulation of inflammatory response to antigenic stimulus positive regulation of activation of JAK2 kinase activity cytokine-mediated signaling pathway sensory perception of pain sexual reproduction natural killer cell activation negative regulation of interleukin-10 production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-17 production positive regulation of tumor necrosis factor production positive regulation of natural killer cell proliferation positive regulation of tissue remodeling regulation of cytokine biosynthetic process T-helper 1 type immune response T-helper cell differentiation interferon-gamma biosynthetic process positive regulation of activated T cell proliferation positive regulation of NF-kappaB import into nucleus regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein positive regulation of memory T cell differentiation negative regulation of growth of symbiont in host positive regulation of interferon-gamma biosynthetic process positive regulation of osteoclast differentiation positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NK T cell proliferation defense response to virus cellular response to interferon-gamma positive regulation of T-helper 17 type immune response positive regulation of T-helper 17 cell lineage commitment
      Process(es) Blue Arrow Icon
      cytokine activity
       interleukin-12 receptor binding
       protein binding
       growth factor activity
       interleukin-12 beta subunit binding
       interleukin-27 binding
       protein heterodimerization activity
       cytokine receptor activity
       interleukin-12 alpha subunit binding
       identical protein binding
       protein homodimerization activity
       interleukin-23 receptor binding

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