Invitrogen

IL-12 p70 Monoclonal Antibody (C11.5)

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IL-12 p70 Antibody in ELISA (ELISA) — ELISA showing IL-12 p70 (Product # AHC9122) compared to a competitor antibody to the same target.

Product Details

AHC9122

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	-	View 2 publications 2 publications
ELISA (ELISA)	1-10 µg/mL	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Non-human primate
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	C11.5
Immunogen	Recombinant human IL-12, p40 expressed in CHO cells.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 µg/mL
Purification	Protein A/G
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	-80°C
RRID	AB_2536295

Product Specific Information

This antibody recognizes the p40 subunit and the p70 heterodimer of human IL-12, and can be used as a capture antibody in ELISA assays. It does not effectively neutralize IL-12.

AHC9122 is >98% pure by SDS-PAGE. Further dilutions should be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA. The addition of a preservative is also recommended for long term storage. For maximal stability, apportion into working aliquots and store at -70°C, avoiding repeated freeze-thaw cycles.

Target Information

Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.

Journal of leukocyte biology

Cox K,North M,Burke M,Singhal H,Renton S,Aqel N,Islam S,Knight SC

AHC9122 was used in flow cytometry to characterize human plasmacytoid dendritic cells from breast cancer patients

Tue Nov 01 00:00:00 EST 2005

Measles virus infection of rhesus macaques affects neutrophil expression of IL-12 and IL-10.

Viral immunology

Hoffman SJ,Polack FP,Hauer DA,Griffin DE

AHC9122 was used in flow cytometry and immunocytochemistry to examine IL-12 and IL-10 production by neutrophils isolated from naive or measles virus-infected rhesus macaques.

Mon Jan 05 00:00:00 EST 2004

Quantification and cytokine production of circulating lymphoid and myeloid cells in acute myelogenous leukaemia.

Leukemia

Panoskaltsis N,Reid CD,Knight SC

AHC9122 was used in Flow cytometry to assess the potential of patients with acute myelogenous leukaemia to respond to immunotherapy.

Tue Apr 01 00:00:00 EST 2003

Altered synthesis of interleukin-12 and type 1 and type 2 cytokinesin rhesus macaques during measles and atypical measles.

The Journal of infectious diseases

Polack FP,Hoffman SJ,Moss WJ,Griffin DE

AHC9122 was used in flow cytometry to investigate the role of IL-12 in measles induced immunosuppression.

Tue Jan 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL35 subunit; ILN; Interleukin; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2

Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35

UniProt ID: (Human) P29459, (Human) P29460

Entrez Gene ID: (Human) 3592, (Human) 3593

Molecular Function: transmembrane signal receptor