|ELISA (ELISA)||1-10 µg/mL|
|Flow Cytometry (Flow)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human IL-12, p40 expressed in CHO cells.|
|Storage buffer||PBS, pH 7.2|
This antibody recognizes the p40 subunit and the p70 heterodimer of human IL-12, and can be used as a capture antibody in ELISA assays. It does not effectively neutralize IL-12.
AHC9122 is >98% pure by SDS-PAGE. Further dilutions should be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA. The addition of a preservative is also recommended for long term storage. For maximal stability, apportion into working aliquots and store at -70°C, avoiding repeated freeze-thaw cycles.
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLMF p35; CLMF p40; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL-12A; IL-12B; IL12, subunit p40; IL12A/B; IL12AB; IL12p35; IL12P40; IL35 subunit; interleukin 12, p35; interleukin 12, p40; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 alpha chain; interleukin-12 beta chain; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF1; NKSF2
Gene Aliases: CLMF; CLMF2; IL-12A; IL-12B; IL12A; IL12B; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.