M130B targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.
The M130B immunogen is recombinant human IL-14. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
M130B detects IL-13 which has a predicted molecular weight of approximately 13 kDa.
The M130B IL13 antibody (biotinylated conjugate of clone 5A2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody P130E. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Biotinylated antibody M130B (clone 5A2) and antibody P130E have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IL-13; Interleukin-13
Gene Aliases: IL-13; IL13; NC30; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596