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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rat / IgG|
|Immunogen||Recombinant human IL-14|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 9 publications below|
M130B targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.
The M130B immunogen is recombinant human IL-14. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
M130B detects IL-13 which has a predicted molecular weight of approximately 13 kDa.
The M130B IL13 antibody (biotinylated conjugate of clone 5A2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody P130E. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M130B (clone 5A2) and antibody P130E have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans.
M130B was used in ELISA to study the role of the interaction between CD226 and CD155 in regulating the human T-cell pro-inflammatory/anti-inflammatory balance
|Lozano E,Joller N,Cao Y,Kuchroo VK,Hafler DA||Journal of immunology (Baltimore, Md. : 1950) (191:3673)||2013|
Cellular immune responses in human immunodeficiency virus (HIV)-1-infected children: is immune restoration by highly active anti-retroviral therapy comparable to non-progression?
M130B was used in ELISA to study cellular immune responses in HIV-1-infected children
|Hainaut M,Verscheure V,Ducarme M,Schanden? L,Levy J,Mascart F||Clinical and experimental immunology (165:77)||2011|
Fibroblasts stimulated via HLA-II molecules produce prostaglandin E? and regulate cytokine production from helper T cells.
M130B was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses
|Kato-Kogoe N,Ohyama H,Nishimura F,Meguro M,Yoshizawa S,Okada Y,Nakasho K,Yamanegi K,Yamada N,Hata M,Higashi T,Terada N,Matsushita S||Laboratory investigation; a journal of technical methods and pathology (90:1747)||2010|
Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya.
M130B was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance
|Malhotra I,Dent A,Mungai P,Wamachi A,Ouma JH,Narum DL,Muchiri E,Tisch DJ,King CL||PLoS medicine (6:null)||2009|
Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.
M130B was used in ELISA to study the mucosal production of interleukin 13 in Helicobacter pylori-related gastric diseases
|Marotti B,Rocco A,De Colibus P,Compare D,de Nucci G,Staibano S,Tatangelo F,Romano M,Nardone G||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (40:240)||2008|
Modulation of the infant immune responses by the first pertussis vaccine administrations.
M130B was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines
|Mascart F,Hainaut M,Peltier A,Verscheure V,Levy J,Locht C||Vaccine (25:391)||2007|
Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands.
M130B was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands
|Malhotra I,Mungai P,Muchiri E,Ouma J,Sharma S,Kazura JW,King CL||Infection and immunity (73:3462)||2005|
Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.
M130B was used in ELISA to investigate the effect of Bordetella pertussis infection on type 1 T cell responses
|Mascart F,Verscheure V,Malfroot A,Hainaut M,Pi?rard D,Temerman S,Peltier A,Debrie AS,Levy J,Del Giudice G,Locht C||Journal of immunology (Baltimore, Md. : 1950) (170:1504)||2003|
Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.
M130B was used in ELISA to study the effect of the attachment (G) glycoprotein from respiratory synctial virus on wheezing
|Hancock GE,Scheuer CA,Sierzega R,Pryharski KS,McBride JT,Watelet LF,Tebbey PW,Smith JD||The Journal of infectious diseases (184:1589)||2001|