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Invitrogen
IL-13 Recombinant Rabbit Monoclonal Antibody (F3B1)
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FIGURE: 1 / 5
IL-13 Antibody (704915) in ICC/IF
Immunofluorescence analysis of IL-13 was performed using IL-13 Recombinant Rabbit Monoclonal Antibody (F3B1) (Product # 704915) on Human PBMCs differentiated to Th2 cells. The cells were fixed with 4% paraformaldehyde for 10 mins, permeabilized with 0.1% Triton™ X-100 for 15 mins and blocked with 2% BSA for 1 hr at room temperature. The cells were labeled with IL-13 Recombinant Rabbit Monoclonal Antibody (F3B1) (Product # 704915, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) ... View More
Product Details
704915
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, lambda
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
F3B1
Immunogen
Protein corresponding to human IL-13 [aa32-aa146]
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Recombinant Rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody
Gene Aliases: IL-13; IL13; NC30; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596
microglial cell activation
positive regulation of immunoglobulin production
movement of cell or subcellular component
inflammatory response
immune response
signal transduction
cell-cell signaling
regulation of proton transport
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of connective tissue growth factor production
negative regulation of NAD(P)H oxidase activity
response to nicotine
positive regulation of tyrosine phosphorylation of Stat6 protein
positive regulation of macrophage activation
positive regulation of mast cell degranulation
response to ethanol
positive regulation of smooth muscle cell proliferation
positive regulation of protein secretion
positive regulation of release of sequestered calcium ion into cytosol
cellular response to mechanical stimulus
cellular response to cytokine stimulus
negative regulation of transforming growth factor beta production
negative regulation of neuron death
negative regulation of lung ciliated cell differentiation
positive regulation of lung goblet cell differentiation
negative regulation of complement-dependent cytotoxicity
positive regulation of pancreatic stellate cell proliferation
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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