Description: The PVM13-1 antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8^+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.
Applications Reported: The PVM13-1 antibody has been reported for use as the capture antibody in an IL-13 ELISPOT assay or ELISA, as well as for neutralization of IL-13 bioactivity.
Applications Tested: The functional grade PVM13-1 antibody has been tested for ELISA and ELISPOT capture and neutralization of IL-13 bioactivity. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ALRH antibody; IL-13; Interleukin-13; NC30 antibody; P600 antibody
Gene Aliases: IL-13; IL13; NC30; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596