Invitrogen

IL-13 Monoclonal Antibody (eBio1316H), Biotin, eBioscience™

Product Image Gallery

IL-13 Antibody in Immunocytochemistry (ICC/IF) — Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Immunocytochemistry

IL-13 Antibody in Immunohistochemistry (IHC) — Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Immunohistochemistry

IL-13 Antibody in Neutralization (Neu) — Figure 4 HA and COVID-19 disease. Mice received i.p. injections of anti-IL-13 on days 0 and 2 after infection, were euthanized on day 5, and lung tissue was split and placed either into trizol tissue reagent for RNA analysis or formaldehyde for paraffin embedding and IHC. ( A ) Gene expression in mouse lung of Has1 , the HA receptor Cd44, and Hyaluronidase 2 ( Has2 ) of infected mice with anti-IL-13, isotype control antibody and uninfected controls (Tukey's HSD). ( B ) Staining of HA in mouse lung (with HABP); Scale bar: 70mum. Rectangle indicates area magnified in image to left; Scale bar: 20mum. ( C ) Quantification of HA deposition in tissue following infection and neutralization of IL-13 (mixed effect model; combined 2 experiments). ( D ) Hyaluronan was measured in the plasma of COVID-19-negative controls and in patients with COVID-19 that did or did not require supplemental oxygen. Postmortem lung samples were obtained from fatal COVID-19 cases and control tissue from COVID-19 negative deaths. ( E ) Quantification of HA deposition in fatal COVID-19 disease ( n = 11) and controls ( n = 8) (log-transformed, mixed effect model) using HABP. ( F ) Representative images of staining for HA (with HABP) from human lung samples; Scale bar: 70mum. Rectangle indicates area magnified in image below; Scale bar: 20mum. Mice were administered anti-CD44 or IgG2 isotype control on each of day 1-4 after infection. ( G ) Kaplan-Meier survival curve (log-rank) and ( H ) Clinical scores for m

Product Details

13-7135-81

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 2 publications 2 publications
ELISA (ELISA)	0.25-1 µg/mL	View 6 publications 6 publications
Neutralization (Neu)	-	View 1 publication 1 publication
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio1316H
Conjugate	Biotin Functional Grade
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_763555

Product Specific Information

Description: The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported: The eBio1316H antibody has been reported useful for neutralization, ELISA detection, and ELISPOT detection.

Applications Tested: The biotinylated eBio1316H antibody has been tested as the detection antibody in a sandwich ELISA (and ELISPOT assay) for analysis of mouse interleukin-13 (IL-13) in combination with the purified eBio13A (14-7133) antibody for capture and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25-1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

For intracellular staining of mouse IL-13, the fluorochrome labelled eBio13A antibody is recommended.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IL-13 is a driver of COVID-19 severity.

JCI insight

Donlan AN,Sutherland TE,Marie C,Preissner S,Bradley BT,Carpenter RM,Sturek JM,Ma JZ,Moreau GB,Donowitz JR,Buck GA,Serrano MG,Burgess SL,Abhyankar MM,Mura C,Bourne PE,Preissner R,Young MK,Lyons GR,Loomba JJ,Ratcliffe SJ,Poulter MD,Mathers AJ,Day AJ,Mann BJ,Allen JE,Petri WA

Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Neutralization

Mon Aug 09 00:00:00 EDT 2021

T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation.

Cell reports

Yan J,Pandey SP,Barnes BJ,Turner JR,Abraham C

13-7135 was used in an ELISA assay to identify a previously undefined key role for T cell-intrinsic IRF5. In mice, IRF5 in CD4+ T cells promotes Th1- and Th17-associated cytokines and decreases Th2-associated cytokines.

Tue Jun 30 00:00:00 EDT 2020

BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice.

JCI insight

Radhakrishnan RK,Thandi RS,Tripathi D,Paidipally P,McAllister MK,Mulik S,Samten B,Vankayalapati R

Published figure using IL-13 monoclonal antibody (Product # 13-7135-81) in Immunocytochemistry

Thu Mar 12 00:00:00 EDT 2020

T cell-intrinsic IL-1R signaling licenses effector cytokine production by memory CD4 T cells.

Nature communications

Jain A,Song R,Wakeland EK,Pasare C

13-7135 was used in Flow cytometry/Cell sorting to study the role of IL-1 as a licensing signal for memory CD4 T cell function.

Thu Aug 09 00:00:00 EDT 2018

IgE promotes type 2 innate lymphoid cells in murine food allergy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Burton OT,Medina Tamayo J,Stranks AJ,Miller S,Koleoglou KJ,Weinberg EO,Oettgen HC

13-7135 was used in an ELISA assay to determine the effect of IgE mediated mast cell activation on intestinal ILC3 responses following ingestion of food allergens.

Thu Mar 01 00:00:00 EST 2018

Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance.

The Journal of allergy and clinical immunology

Burton OT,Tamayo JM,Stranks AJ,Koleoglou KJ,Oettgen HC

13-7135 was used in an ELISA assay to suggest that allergen-specific IgG antibodies can act to induce and sustain immunologic tolerance to foods.

Mon Jan 01 00:00:00 EST 2018

Intratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

Immunity, inflammation and disease

Edukulla R,Rehn KL,Liu B,McAlees JW,Hershey GK,Wang YH,Lewkowich I,Lindsley AW

13-7135 was used in an ELISA assay to evaluate the immune regulatory role of de novo ceramide generation in vitro and in vivo.

Thu Sep 01 00:00:00 EDT 2016

Interleukin 13- and interleukin 17A-induced pulmonary hypertension phenotype due to inhalation of antigen and fine particles from air pollution.

Pulmonary circulation

Park SH,Chen WC,Esmaeil N,Lucas B,Marsh LM,Reibman J,Grunig G

Mon Dec 01 00:00:00 EST 2014

Signaling pathways activated by a protease allergen in basophils.

Proceedings of the National Academy of Sciences of the United States of America

Rosenstein RK,Bezbradica JS,Yu S,Medzhitov R

13-7135 was used in Flow cytometry/Cell sorting to study signaling pathways activated by a protease allergen in basophils.

Tue Nov 18 00:00:00 EST 2014

Group 2 innate lymphoid cells facilitate sensitization to local, but not systemic, TH2-inducing allergen exposures.

The Journal of allergy and clinical immunology

Gold MJ,Antignano F,Halim TY,Hirota JA,Blanchet MR,Zaph C,Takei F,McNagny KM

13-7135 was used in an ELISA assay to reveal a critical role for ILC2s in the development of adaptive type 2 responses to local antigen exposure.

Tue Apr 01 00:00:00 EDT 2014

Contribution of IL-13 to early exocrinopathy in Id3-/- mice.

Molecular immunology

Mahlios J,Zhuang Y

13-7135 was used in Immunofluorescence to examine the role of cytokines produced by T cells in the systemic regulation of salivary gland function.

Sat Oct 01 00:00:00 EDT 2011

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

13-7135 was used in an ELISA assay to suggest that major histocompatibility complex class II-dependent interactions between basophils and CD4+ T cells promote T helper type 2 cytokine responses and immunity to helminth infection.

Wed Jul 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

Gene Aliases: Il-13; Il13

UniProt ID: (Mouse) P20109

Entrez Gene ID: (Mouse) 16163

Function(s)