Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
- | View 2 publications 2 publications |
ELISA (ELISA) |
0.25-1 µg/mL | View 6 publications 6 publications |
Neutralization (Neu) |
- | View 1 publication 1 publication |
Functional Assay (FN) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Rat / IgG1, kappa |
Recommended Isotype Control |
Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
eBio1316H |
Conjugate |
Biotin
|
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, do not freeze |
RRID |
AB_763555 |
Description: The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.
Applications Reported: The eBio1316H antibody has been reported useful for neutralization, ELISA detection, and ELISPOT detection.
Applications Tested: The biotinylated eBio1316H antibody has been tested as the detection antibody in a sandwich ELISA (and ELISPOT assay) for analysis of mouse interleukin-13 (IL-13) in combination with the purified eBio13A (14-7133) antibody for capture and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25-1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
For intracellular staining of mouse IL-13, the fluorochrome labelled eBio13A antibody is recommended.
Filtration: 0.2 µm post-manufacturing filtered.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600
Gene Aliases: Il-13; Il13
UniProt ID: (Mouse) P20109
Entrez Gene ID: (Mouse) 16163
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support