BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified, soluble Anti-Mouse CD28 Functional Grade Purified, Mouse IL-2 Recombinant Protein, and Mouse IL-4 Recombinant Protein. Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized then stained with Anti-Mouse CD4 FITC and 0.125 ug of Rat IgG1 K Isotype Control PE or 0.125 ug of Anti-Mouse IL-13 PE (A18492). Viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Mouse IL-13 Protein|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IL-13 is a cytokine involved in regulating inflammatory and immune responses. It down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.