|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant, full length human IL15 protein expressed in E. coli.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||0.05-0.10 ug/ml|
|Neutralization (Neu)||0.05-0.1 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
MA1-24810 detects IL15 from human samples.
MA1-24810 has been successfully used in neutralization, inhibition assays, and Western blot procedures. Neutralization: Use at a concentration of 0.05-0.1 ug/ml in the presence of 10 ng/ml of recombinant human IL15 using the MO7e cell line.
The MA1-24810 immunogen is recombinant, full length human IL15 protein expressed in E. coli.
The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.