|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL-17|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5 - 2.0 ug/ml|
|Functional Assay (FN)||0.93 - 1.3 µg/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||0.25 ug/ml|
|Western Blot (WB)||0.1 - 0.2ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. This antibody may be used in Western Blotting applications under either reducing or non-reducing conditions.
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended (unless the product will be used for functional studies). Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
IL-17 (Interleukin-17/17A) is a 32kDa disulfide-linked homodimer consisting of two 136 amino acid chains, which belongs to the IL-17 family (classified as IL-17A to IL-17F), and signals through the IL-17 receptor (IL-17R/CDw217). IL-17 is produced primarily by activated CD4+ T cells and induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNFalpha, IFNgamma and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. The role of IL-17 in bone metabolism has become a focus area for studies into Rheumatoid Arthritis, in which levels of IL-17 are significantly increased and may contribute to bone destruction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.