Invitrogen

IL-17A Polyclonal Antibody

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This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.

3 Published Figures

1 Reference

View all (117) IL-17A antibodies

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Protocols

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IL-17A Antibody in Flow Cytometry (Flow) — Flow cytometry of IL-17A in EL-4 mouse lymphoblast cell line was treated for 16 hours with 50 ng/mL PMA. Samples were incubated in IL-17A polyclonal antibody (Product # PA5-46947) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

IL-17A Antibody in Neutralization (Neu) — Neutralization of IL-17A in NIH-3T3 mouse embryonic fibroblast cell line. Samples were incubated in IL-17A polyclonal antibody (Product # PA5-46947). Recombinant Mouse IL-17 stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit. IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.05-0.25 µg/mL.

IL-17A Antibody in Western Blot (WB) — Figure 5 PM 2.5 elevates the proliferation in NSCLC cells. ( A - E ) Th17 cells were treated with 100 mug/cm 2 of PM 2.5 for 24 h, and then all cells and supernatants were collected for the analysis. ( A ) TNF-alpha and IL-6 levels in the supernatants were measured using ELISA. ( B ) IL-17a contents in the collected supernatants were calculated using ELISA. ( C ) RT-qPCR and ( D ) western blot analysis were used to measure IL-17a expression levels in the harvested cells. ( E ) IF staining of IL-17a in the harvested cells. Scale bar, 20 mum. ( F ) Th17 cells were treated with 100 mug/cm 2 of PM 2.5 for 24 h, and then the conditional medium was collected, and mixed with fresh RPMI1640 absolute medium at 1:3. The composed culture medium was exposed to A549 and H1350 cells for 12, 24, 48 or 72 h. Then, the NSCLC cells were collected for cell proliferation analysis using CCK-8 analysis. ( G , H ) Th17 cells were incubated with 100 mug/cm 2 of PM 2.5 for 24 h, and then the conditional medium was collected, and mixed with fresh RPMI1640 absolute medium at 1:3. Then, the composed culture medium was subjected to A549 and H1350 cells for another 24 h. Subsequently, all cells were harvested to assess the cell proliferation using colony formation and EdU assays. Scale bar, 100 mum. Quantification of colony formation assay and EdU was exhibited. All data are expressed as mean +- SEM. * p<0.05 and ** p<0.01 compared to the Ctrl group.

IL-17A Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of mouse IL-17 by Product # PA5-46947 shows decrease in OD (measure of IL-6 secretion) with increasing concentrations of IL-17A polyclonal antibody. {Neu}

Product Details

PA5-46947

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	View 1 publication 1 publication
Flow Cytometry (Flow)	2.5 µg per million cells	-
Neutralization (Neu)	0.05-0.25 µg/mL	-

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived recombinant mouse IL-17 Thr22-Ala158 View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2607353

Product Specific Information

In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17E is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long-term PM_2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis.

Aging

Chao X,Yi L,Lan LL,Wei HY,Wei D

PA5-46947 was used in Western Blotting to explore the role of PM2.5 in the etiology of NSCLC.

Thu Jun 18 00:00:00 EDT 2020

Long-term PM_2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis.

Aging

Chao X,Yi L,Lan LL,Wei HY,Wei D

PA5-46947 was used in Western Blotting to explore the role of PM2.5 in the etiology of NSCLC.

Thu Jun 18 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin-17A; Interleukin17A; R-IL-17-A

Gene Aliases: Ctla-8; Ctla8; IL-17; IL-17A; Il17; Il17a

UniProt ID: (Mouse) Q62386

Entrez Gene ID: (Mouse) 16171

Function(s)

inflammatory response positive regulation of necrotic cell death positive regulation of interleukin-23 production positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to interleukin-1 cellular response to glucocorticoid stimulus fibroblast activation positive regulation of cytokine production involved in inflammatory response

Process(es)

cytokine activity

IL-17A Polyclonal Antibody

Product Details

PA5-46947

Western Blot (WB)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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