Invitrogen

IL-17A Monoclonal Antibody (eBioTC11-18H10.1), Alexa Fluor™ 488, eBioscience™

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IL-17A Antibody in Flow Cytometry (Flow) — Intracellular staining of CD4+CD25- T cells purified from C57BL/6 mouse splenocytes cultured with 10-day DCs in the presence of Anti-Mouse CD3 Functional Grade Purified (Product # 16-0031-82), Mouse IL-4, IL-6 and TGF beta Recombinant Proteins for 4 days. Cells were restimulated with PMA/Ionomycin in the presence of monensin for 5 hrs, then fixed, permeabilized, and stained with 0.2 µg of Anti-Mouse IL-17A Alexa Fluor® 488 and 0.25 µg of Anti-Mouse IFN gamma APC. (Left figure - no reactivation; Right figure - reactivation).

Product Details

53-7172-80

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.50 µg/test	View 1 publication 1 publication
Inhibition Assays (IA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBioTC11-18H10.1
Conjugate	Alexa Fluor™ 488
Excitation/Emission Max	499/520 nm View spectra
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_493960

Product Specific Information

Description: The eBioTC11-18H10.1 antibody reacts with mouse IL-17A. The eBioTC11-18H10.1 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Applications Reported: The eBioTC11-18H10.1 antibody has been reported for use as the capture antibody in mouse IL-17A ELISA and ELISPOT assay, for neutralization of IL-17A bioactivity, and for intracellular staining of IL-17A-producing cells for flow cytometric analysis.

Applications Tested: The fluorochrome-conjugated eBioTC11-18H10.1 antibody has been tested by intracellular staining for flow cytometric analysis and can be used at less than or equal to 0.50 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Regulatory T Cells Control Th2-Dominant Murine Autoimmune Gastritis.

Journal of immunology (Baltimore, Md. : 1950)

Harakal J,Rival C,Qiao H,Tung KS

53-7172-80 was used in Flow Cytometry to suggest that Tregs maintain physiological tolerance to clinically relevant gastric autoantigens, and Th2 responses can be a pathogenic mechanism in AIG.

Fri Jul 01 00:00:00 EDT 2016

Ozone exposure in a mouse model induces airway hyperreactivity that requires the presence of natural killer T cells and IL-17.

The Journal of experimental medicine

Pichavant M,Goya S,Meyer EH,Johnston RA,Kim HY,Matangkasombut P,Zhu M,Iwakura Y,Savage PB,DeKruyff RH,Shore SA,Umetsu DT

53-7172 was used in Blocking experiments to suggest that NKT cells mediate a unifying pathogenic mechanism for several distinct forms of asthma.

Mon Feb 18 00:00:00 EST 2008

Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin-17A; Interleukin17A; R-IL-17-A

Gene Aliases: Ctla-8; Ctla8; IL-17; IL-17A; Il17; Il17a

UniProt ID: (Mouse) Q62386

Entrez Gene ID: (Mouse) 16171

Function(s)

inflammatory response positive regulation of necrotic cell death positive regulation of interleukin-23 production positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to interleukin-1 cellular response to glucocorticoid stimulus fibroblast activation positive regulation of cytokine production involved in inflammatory response

Process(es)

cytokine activity

IL-17A Monoclonal Antibody (eBioTC11-18H10.1), Alexa Fluor™ 488, eBioscience™

Product Details

53-7172-80

Flow Cytometry (Flow)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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