Sandwich ELISA analysis of Mouse IL-2 MAb (Pierce product # MM100) was performed using the Thermo Fisher Scientific Mouse IL-2 Colorimetric ELISA Kit (Pierce product # EMIL2) by loading 50 µl per well of Mouse IL-2 MAb (MM100) in quadruplicate at 0, 50% (2.5 µg/ml), and 100% (5 µg/ml) displacement in a plate reagent and then loading 50ul per well of Recombinant Mouse IL-2 (SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/ml across a 5ug/ml, Mouse IL-2 MAb (Pierce product # MM101) pre-coated plate and incubated for 2 hours at 37°C. The plate was washed then incubated with 100ul per well of Rat anti-Mouse IL-2 HRP labeled stock at 1:25,000 for 1 hour at 37°C. The plate was washed and incubated with 100ul per well of TMB substrate solution for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550nm.
|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse IL-2|
|Purification||Caprylic acid precipitation|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM100 targets IL-2 in Western blot and ELISA applications and shows reactivity with mouse samples.
The MM100 immunogen is recombinant mouse IL-2.
MM100 detects IL-2 which has a predicted molecular weight of approximately 17 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.
MM100 was used in ELISA to investigate the changes of cytokine expression in NK cells after interacting with tumor cells
|Das S,Varalakshmi C,Kumari AL,Patel M,Khar A||Cancer immunology, immunotherapy : CII (50:428)||2001|
Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.
MM100 was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression
|Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M||The Journal of pharmacology and experimental therapeutics (286:767)||1998|
Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.
MM100 was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system
|Ichinose T,Takano H,Miyabara Y,Sagai M||Toxicological sciences : an official journal of the Society of Toxicology (44:70)||1998|
Identification of the immunodominant T cell epitope of p38, a major egg antigen, and characterization of the epitope-specific Th responsiveness during murine schistosomiasis mansoni.
MM100 was used in ELISA to investigate the immunodominant epitope of p38 and the production of Th-derived cytokine during murine Schistosomiasis mansoni infection
|Chen Y,Boros DL||Journal of immunology (Baltimore, Md. : 1950) (160:5420)||1998|
Murine strain differences in airway inflammation caused by diesel exhaust particles.
MM100 was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants
|Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M||The European respiratory journal (11:291)||1998|
Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.
MM100 was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression
|Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M||American journal of respiratory and critical care medicine (156:36)||1997|
Functionally anergic lpr and gld B220+ T cell receptor (TCR)-alpha/beta+ double-negative T cells express CD28 and respond to costimulation with phorbol myristate acetate and antibodies to CD28 and the TCR.
MM100 was used in ELISA to study the mechanism for the immunosuppression of specific T cell subpopulation
|Giese T,Allison JP,Davidson WF||Journal of immunology (Baltimore, Md. : 1950) (151:597)||1993|
IL-4 enhances long-term survival of CD28-deficient T cells.
MM100 was used in blocking/activating experiment to investigate the effect of interleukin 4 on T cel survival
|Stack RM,Thompson CB,Fitch FW||Journal of immunology (Baltimore, Md. : 1950) (160:2255)||1998|
Requirement for in vivo production of IL-4, but not IL-10, in the induction of proliferative suppression by filarial parasites.
MM100 was used in blocking/activating experiment to investigate the mechanism for the anti-proliferative activity of filarial parasites
|MacDonald AS,Maizels RM,Lawrence RA,Dransfield I,Allen JE||Journal of immunology (Baltimore, Md. : 1950) (160:1304)||1998|
A role for nonspecific (cyclosporin A) or specific (monoclonal antibodies to ICAM-1, LFA-1, and IL-10) immunomodulation in the prolongation of skin allografts after antigen-specific pretransplant immunization or transfusion.
MM100 was used in blocking/activating experiment to study the approaches to achieve long-term graft survival in mice
|Gorczynski RM,Wojcik D||Journal of immunology (Baltimore, Md. : 1950) (152:2011)||1994|
Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.
MM100 was used in immunohistochemistry to investigate the production of interleukin 2 and 4 and interferon gamma in mice immunized with protein antigens
|Bogen SA,Fogelman I,Abbas AK||Journal of immunology (Baltimore, Md. : 1950) (150:4197)||1993|