Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

16 Published Figures

34 References

View all (105) IL-2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IL-2 Antibody in Flow Cytometry (Flow) — FIG 6 mtdVSV-based SARS-CoV-2 vaccines induce strong Th1-biased T-cell immune responses. The 5 female mice from each group shown in Fig. 5 were terminated at week 4 postimmunization for the T-cell assays. (A) Enzyme-linked immunosorbent spot assay (ELISPOT) quantification of IFN-gamma-producing T cells. Spot-forming cells (SFC) were quantified after the cells were stimulated by peptides representing N (S1 peptides, pink) and C (S2 peptides, green) termini of the SARS-CoV-2 spike protein. A peptide pool of covering N protein SARS-CoV-2 (N peptide) was used as a control. Data are means of 5 mice +- standard deviation. * , P < 0.05; * * , P < 0.01, determined by unpaired t test. (B) Intracellular cytokine staining of T cells in vaccinated mice. Splenocytes of 4 rVSV-D1762A-S (left) or 4 rVSV-D1762A-S1 (right) vaccinated mice were stimulated ex vivo for 5 h with pools of N, S1, or S2 peptides (5 mug/ml each), followed by staining for intracellular cytokines and analysis using multicolor flow cytometry. Frequencies of CD8 + T cells expressing cytokines represent CD8 + T cells expressing IFN-gamma, TNF-alpha, IL-2, IL-17a, IL-21, IL-4, or IL-10. Data are means of 5 mice +- standard deviation and were analyzed using an unpaired t test ( * , P < 0.05). (C) Flow plots showing T cells producing the three Th1 cytokines. Splenocytes of rVSV-D1762A-S-vaccinated (upper) and rVSV-D1762A-S1-vaccinated (lower) mice were stimulated with either S1 peptides or no peptides (one representative plo

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

12-7021-41

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 31 publications 31 publications
In Situ Hybridization (ISH) (ISH)	-	View 1 publication 1 publication
ELISA (ELISA)	-	View 1 publication 1 publication
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES6-5H4
Conjugate	PE Alexa Fluor 488 APC APC-eFluor 780 Biotin Brilliant UV 737 eFluor 450 FITC Functional Grade PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Unconjugated
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_11042257

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: JES6-5H4 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This JES6-5H4 antibody has been pre-titrated and tested ny intracellular staining of 6-hour PMA and Ionomycin-activated mouse splenocytes. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8⁺ T Cells in Non-small Cell Lung Cancer.

Frontiers in oncology

Ye J,Zou MM,Li P,Lin XJ,Jiang QW,Yang Y,Huang JR,Yuan ML,Xing ZH,Wei MN,Li Y,Shi Z,Liu H

12-7021-41 was used in Flow Cytometry to demonstrate that the combination of OMT with cisplatin (DDP) synergistically inhibited non-small cell lung cancer (NSCLC) cells growth when co-cultured with peripheral blood mononuclear cells in vitro.

Thu Oct 01 00:00:00 EDT 2020

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death.

Nature communications

Sasaki K,Himeno A,Nakagawa T,Sasaki Y,Kiyonari H,Iwai K

12-7021-41 was used in Flow Cytometry to show that T cell-mediated killing and sequential autoinflammation are common and crucial for pathogenic diversity during T cell-mediated autoimmune responses.

Wed Aug 28 00:00:00 EDT 2019

Costimulation through TLR2 Drives Polyfunctional CD8⁺ T Cell Responses.

Journal of immunology (Baltimore, Md. : 1950)

Salerno F,Freen-van Heeren JJ,Guislain A,Nicolet BP,Wolkers MC

Fri Feb 01 00:00:00 EST 2019

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

TGF-β receptor maintains CD4 T helper cell identity during chronic viral infections.

The Journal of clinical investigation

Lewis GM,Wehrens EJ,Labarta-Bajo L,Streeck H,Zuniga EI

12-7021 was used in Flow cytometry/Cell sorting to uncover an eomesodermin-driven CD4 T cell program that is continuously suppressed by TGF-β signalling.

Mon Oct 03 00:00:00 EDT 2016

Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells.

PLoS pathogens

Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ

12-7021 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.

Fri Jul 01 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 12-7021-41) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.

Scientific reports

Ying W,Tseng A,Chang RC,Wang H,Lin YL,Kanameni S,Brehm T,Morin A,Jones B,Splawn T,Criscitiello M,Golding MC,Bazer FW,Safe S,Zhou B

12-7021 was used in Flow cytometry/Cell sorting to demonstrate a role for miR-150 in regulating B cell functions in adipose tissue.

Mon Feb 01 00:00:00 EST 2016

mTOR masters monocytic myeloid-derived suppressor cells in mice with allografts or tumors.

Scientific reports

Wu T,Zhao Y,Wang H,Li Y,Shao L,Wang R,Lu J,Yang Z,Wang J,Zhao Y

12-7021 was used in Flow cytometry/Cell sorting to offer evidence supporting that mTOR is an intrinsic factor essential for the differentiation and immunosuppressive function of M-MDSCs.

Mon Feb 01 00:00:00 EST 2016

A new recombinant BCG vaccine induces specific Th17 and Th1 effector cells with higher protective efficacy against tuberculosis.

PloS one

da Costa AC,Costa-Júnior Ade O,de Oliveira FM,Nogueira SV,Rosa JD,Resende DP,Kipnis A,Junqueira-Kipnis AP

12-7021 was used in Flow cytometry/Cell sorting to evaluate the immunogenicity and protection of a new BCG vaccine against TB, showing that it induces specific Th17 and Th1 effector cells with higher protective efficacy.

Fri Dec 18 00:00:00 EST 2015

Eliminating roles for T-bet and IL-2 but revealing superior activation and proliferation as mechanisms underpinning dominance of regulatory T cells in tumors.

Oncotarget

Colbeck EJ,Hindley JP,Smart K,Jones E,Bloom A,Bridgeman H,McPherson RC,Turner DG,Ladell K,Price DA,O'Connor RA,Anderton SM,Godkin AJ,Gallimore AM

12-7021 was used in Flow cytometry/Cell sorting to study the enrichment of tumor-infiltrating Treg populations and assess the role of IL-2 in pertubation of Treg frequencies in tumors.

Tue Sep 22 00:00:00 EDT 2015

IFN-β Selectively Inhibits IL-2 Production through CREM-Mediated Chromatin Remodeling.

Journal of immunology (Baltimore, Md. : 1950)

Otero DC,Fares-Frederickson NJ,Xiao M,Baker DP,David M

12-7021 was used in Flow cytometry/Cell sorting to investigate the mechanism by which interferon beta limits T cell responses and its potential therapeutic use for multiple sclerosis.

Mon Jun 01 00:00:00 EDT 2015

Immunosuppression by N-methyl-D-aspartate receptor antagonists is mediated through inhibition of Kv1.3 and KCa3.1 channels in T cells.

Molecular and cellular biology

Kahlfuß S,Simma N,Mankiewicz J,Bose T,Lowinus T,Klein-Hessling S,Sprengel R,Schraven B,Heine M,Bommhardt U

12-7021 was used in Flow cytometry/Cell sorting to investigate the effect of NMDAR antagonists on T-cell function, showing that antagonists cause immunosuppression via inhibition of Kv1.3 and KCa3.1 channels in T-cells.

Sat Mar 01 00:00:00 EST 2014

ZBTB1 is a determinant of lymphoid development.

The Journal of experimental medicine

Siggs OM,Li X,Xia Y,Beutler B

12-7021 was used in Flow cytometry/Cell sorting to identify a mutation causative of complete and cell-intrinsic T cell deficiency.

Mon Jan 16 00:00:00 EST 2012

IL-1β promotes TGF-β1 and IL-2 dependent Foxp3 expression in regulatory T cells.

PloS one

Ganesh BB,Bhattacharya P,Gopisetty A,Sheng J,Vasu C,Prabhakar BS

12-7021 was used in an ELISA assay to investigate the differential effects of IL-12 and IL-1β on Foxp3 expression in T cells, showing that IL-1β promotes TGF-β1- and IL-2-dependent Foxp3 expression in Tregs.

Tue Nov 15 00:00:00 EST 2011

Combining liver- and blood-stage malaria viral-vectored vaccines: investigating mechanisms of CD8+ T cell interference.

Journal of immunology (Baltimore, Md. : 1950)

Forbes EK,Biswas S,Collins KA,Gilbert SC,Hill AV,Draper SJ

12-7021 was used in Flow cytometry/Cell sorting to investigate the various mechanisms of CD8(+) T cell interference and the implications for the development of a multistage or multicomponent viral vectored malaria vaccine for use in humans.

Sat Oct 01 00:00:00 EDT 2011

Cutting edge: attrition of Plasmodium-specific memory CD8 T cells results in decreased protection that is rescued by booster immunization.

Journal of immunology (Baltimore, Md. : 1950)

Schmidt NW,Harty JT

12-7021 was used in Flow cytometry/Cell sorting to investigate whether unrelated infections will compromise numbers of Plasmodium-specific memory CD8 T cells, showing that attrition of Plasmodium-specific cells is rescued by booster immunisation.

Fri Apr 01 00:00:00 EDT 2011

CD8+CD122+ regulatory T cells (Tregs) and CD4+ Tregs cooperatively prevent and cure CD4+ cell-induced colitis.

Journal of immunology (Baltimore, Md. : 1950)

Endharti AT,Okuno Y,Shi Z,Misawa N,Toyokuni S,Ito M,Isobe K,Suzuki H

12-7021 was used in Flow cytometry/Cell sorting to investigate the role of CD8(+)CD122(+) regulatory T cells (Tregs) in maintenance of immune homeostasis, showing that CD8(+)CD122(+) Tregs and CD4+ Tregs cooperatively prevent and cure CD4+ cell-induced colitis.

Sat Jan 01 00:00:00 EST 2011

Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg.

European journal of immunology

Shi Z,Okuno Y,Rifa'i M,Endharti AT,Akane K,Isobe K,Suzuki H

12-7021-41 was used in Flow Cytometry to suggest that human CD8(+)CXCR3(+) T cells are the counterparts of murine CD8(+)CD122(+) Treg.

Sat Aug 01 00:00:00 EDT 2009

Cross-presentation of male seminal fluid antigens elicits T cell activation to initiate the female immune response to pregnancy.

Journal of immunology (Baltimore, Md. : 1950)

Moldenhauer LM,Diener KR,Thring DM,Brown MP,Hayball JD,Robertson SA

12-7021 was used in Flow cytometry/Cell sorting to study how at conception, seminal fluid drives activation and expansion of paternal Ag-reactive CD4(+) and CD8(+) T cell populations.

Mon Jun 15 00:00:00 EDT 2009

Postthymic maturation influences the CD8 T cell response to antigen.

Proceedings of the National Academy of Sciences of the United States of America

Makaroff LE,Hendricks DW,Niec RE,Fink PJ

12-7021 was used in Flow cytometry/Cell sorting to demonstrate that the post-thymic maturation stage influences cell fate decisions and cytokine profiles of stimulated CD8 T-cells.

Tue Mar 24 00:00:00 EDT 2009

Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Lal G,Zhang N,van der Touw W,Ding Y,Ju W,Bottinger EP,Reid SP,Levy DE,Bromberg JS

12-7021 was used in Functional assays to investigate Foxp3 as the master switch for Tregs, showing that expression is regulated by DNA methylation in Tregs.

Thu Jan 01 00:00:00 EST 2009

Dicer-dependent microRNA pathway safeguards regulatory T cell function.

The Journal of experimental medicine

Liston A,Lu LF,O'Carroll D,Tarakhovsky A,Rudensky AY

12-7021 was used in Flow cytometry/Cell sorting to study regulatory T cell-mediated tolerance, showing that it is dependent on the Dicer-controlled microRNA pathway.

Mon Sep 01 00:00:00 EDT 2008

Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.

International immunology

Ito H,Ando K,Ishikawa T,Nakayama T,Taniguchi M,Saito K,Imawari M,Moriwaki H,Yokochi T,Kakumu S,Seishima M

12-7021 was used in Flow cytometry/Cell sorting to open up a new method for clearing the virus from patients with persistent HBV infection.

Tue Jul 01 00:00:00 EDT 2008

TRAIL deficiency does not rescue impaired CD8+ T cell memory generated in the absence of CD4+ T cell help.

Journal of immunology (Baltimore, Md. : 1950)

Sacks JA,Bevan MJ

12-7021 was used in Flow cytometry/Cell sorting to investigate the role of the proapoptotic molecule TRAIL in unhelped CD8(+) T cells.

Tue Apr 01 00:00:00 EDT 2008

The surprising kinetics of the T cell response to live antigenic cells.

Journal of immunology (Baltimore, Md. : 1950)

Tyznik AJ,Bevan MJ

12-7021 was used in Flow cytometry/Cell sorting to demonstrate dramatic differences in host T cell responses to noninflammatory Ags compared with responses to pathogens.

Mon Oct 15 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

Gene Aliases: Il-2; Il2

UniProt ID: (Mouse) P04351

Entrez Gene ID: (Mouse) 16183

Molecular Function: actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Function(s)

Process(es)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

IL-2 Monoclonal Antibody (JES6-5H4), PE, eBioscience™

Product Details

12-7021-41

Flow Cytometry (Flow)

In Situ Hybridization (ISH) (ISH)

ELISA (ELISA)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!