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Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.
Applications Reported: The functional grade purified JES6-5H4 has been reported for use in blocking of fluorochrome conjugated JES6-5H4 in intracellular flow cytometry experiments to serve as specificity control. JES6-5H4 is reported for detection of mouse IL-2 by ELISA, immunoprecipitation, and for neutralizing IL-2 binding and biological activity.
Applications Tested: The JES6-5H4 antibody has been tested by sandwich ELISA for detection of mouse IL-2 in combination with JES6-1A12 and can be used between 0.5-2 µg/mL.
Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL-2; Interleukin-2; T-cell growth factor; TCGF