Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), Functional Grade, eBioscience™

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

22 Published Figures

20 References

View all (105) IL-2 antibodies

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IL-2 Antibody in Flow Cytometry (Flow) — FIG 6 mtdVSV-based SARS-CoV-2 vaccines induce strong Th1-biased T-cell immune responses. The 5 female mice from each group shown in Fig. 5 were terminated at week 4 postimmunization for the T-cell assays. (A) Enzyme-linked immunosorbent spot assay (ELISPOT) quantification of IFN-gamma-producing T cells. Spot-forming cells (SFC) were quantified after the cells were stimulated by peptides representing N (S1 peptides, pink) and C (S2 peptides, green) termini of the SARS-CoV-2 spike protein. A peptide pool of covering N protein SARS-CoV-2 (N peptide) was used as a control. Data are means of 5 mice +- standard deviation. * , P < 0.05; * * , P < 0.01, determined by unpaired t test. (B) Intracellular cytokine staining of T cells in vaccinated mice. Splenocytes of 4 rVSV-D1762A-S (left) or 4 rVSV-D1762A-S1 (right) vaccinated mice were stimulated ex vivo for 5 h with pools of N, S1, or S2 peptides (5 mug/ml each), followed by staining for intracellular cytokines and analysis using multicolor flow cytometry. Frequencies of CD8 + T cells expressing cytokines represent CD8 + T cells expressing IFN-gamma, TNF-alpha, IL-2, IL-17a, IL-21, IL-4, or IL-10. Data are means of 5 mice +- standard deviation and were analyzed using an unpaired t test ( * , P < 0.05). (C) Flow plots showing T cells producing the three Th1 cytokines. Splenocytes of rVSV-D1762A-S-vaccinated (upper) and rVSV-D1762A-S1-vaccinated (lower) mice were stimulated with either S1 peptides or no peptides (one representative plo

IL-2 Antibody in ELISA (ELISA) — Figure 3 CIC negatively regulates T-cell activation. ( a , b ) Increased CIC expression in activated CD4 + T cells. Western blot analysis for CIC levels in CD4 + T cells during T-cell activation by alpha-CD3/CD28 antibodies ( a ) and in sorted naive (CD44 lo CD62L hi ) and effector/memory (CD44 hi CD62L lo ) CD4 + T cells ( b ). ( c ) ELISA of IL-2. WT and Cic -deficient (cKO) CD4 + CD25 - CD44 lo CD62L hi naive T cells were stimulated with plate-bound anti-CD3 (1.0 mug ml -1 ) in the presence (right) or absence (left) of plate-bound anti-CD28 (2.0 mug ml -1 ). The supernatants were taken 48 h and 72 h after stimulation and subjected to ELISA for IL-2 concentration. n =4 per each sample. Error bars indicate s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 (two-tailed two-sample unequal variance Student t -test). ( d ) In vitro T-cell proliferation assay. Naive CD4 + T cells purified from pooled spleens and lymph nodes of Cic f/f and Cic f/f Vav1-Cre mice were labelled with CTV dye and stimulated with plate-bound anti-CD3 (1.0 mug ml -1 ) in the presence (right) or absence (left) of plate-bound anti-CD28 (2.0 mug ml -1 ). The cells were analysed 72 h after stimulation. Data are representative of three independent experiments. Shaded area: unstimulated control, blue line: Cic f/f , red line: Cic f/f Vav1-Cre .

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

16-7021-85

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	Assay-Dependent	View 15 publications 15 publications
ELISA (ELISA)	0.5-2 µg/mL	View 4 publications 4 publications
Functional Assay (FN)	Assay-Dependent	-
In vitro Assay (IV)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES6-5H4
Conjugate	Functional Grade Alexa Fluor 488 APC APC-eFluor 780 Biotin Brilliant UV 737 eFluor 450 FITC PE PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_657802

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: The functional grade purified JES6-5H4 has been reported for use in blocking of fluorochrome conjugated JES6-5H4 in intracellular flow cytometry experiments to serve as specificity control. JES6-5H4 is reported for detection of mouse IL-2 by ELISA, immunoprecipitation, and for neutralizing IL-2 binding and biological activity.

Applications Tested: The JES6-5H4 antibody has been tested by sandwich ELISA for detection of mouse IL-2 in combination with JES6-1A12 and can be used between 0.5-2 µg/mL.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Oxymatrine and Cisplatin Synergistically Enhance Anti-tumor Immunity of CD8⁺ T Cells in Non-small Cell Lung Cancer.

Frontiers in oncology

Ye J,Zou MM,Li P,Lin XJ,Jiang QW,Yang Y,Huang JR,Yuan ML,Xing ZH,Wei MN,Li Y,Shi Z,Liu H

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.

Nature communications

Park S,Lee S,Lee CG,Park GY,Hong H,Lee JS,Kim YM,Lee SB,Hwang D,Choi YS,Fryer JD,Im SH,Lee SW,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Wed Jul 12 00:00:00 EDT 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.

Scientific reports

Ying W,Tseng A,Chang RC,Wang H,Lin YL,Kanameni S,Brehm T,Morin A,Jones B,Splawn T,Criscitiello M,Golding MC,Bazer FW,Safe S,Zhou B

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition.

Proceedings of the National Academy of Sciences of the United States of America

Limon JJ,So L,Jellbauer S,Chiu H,Corado J,Sykes SM,Raffatellu M,Fruman DA

16-7021 was used in in vitro experiments to further elucidate the complexity of mTOR signalling in B cells.

Tue Nov 25 00:00:00 EST 2014

Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.

PLoS neglected tropical diseases

Roupie V,Pidot SJ,Einarsdottir T,Van Den Poel C,Jurion F,Stinear TP,Huygen K

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Mon Sep 08 00:00:00 EDT 2014

Sterol regulatory element-binding proteins are essential for the metabolic programming of effector T cells and adaptive immunity.

Nature immunology

Kidani Y,Elsaesser H,Hock MB,Vergnes L,Williams KJ,Argus JP,Marbois BN,Komisopoulou E,Wilson EB,Osborne TF,Graeber TG,Reue K,Brooks DG,Bensinger SJ

16-7021 was used in Flow cytometry/Cell sorting to provide insights into the molecular signals that underlie the metabolic reprogramming of CD8(+) T cells during the transition from quiescence to activation.

Wed May 01 00:00:00 EDT 2013

Environmental effects on protection against Mycobacterium tuberculosis after immunization with Ad85A.

Vaccine

Beverley P,Ronan E,Lee L,Arnold I,Bolinger B,Powrie F,Tchilian E

16-7021 was used in Flow cytometry/Cell sorting to show that exposure to environmental microorganisms can alter the protective immune response to a parenterally administered subunit vaccine.

Mon Feb 04 00:00:00 EST 2013

Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines.

Blood

Ahonen CL,Wasiuk A,Fuse S,Turk MJ,Ernstoff MS,Suriawinata AA,Gorham JD,Kedl RM,Usherwood EJ,Noelle RJ

16-7021 was used in Flow cytometry/Cell sorting to report the therapeutic effects of combinatorial use of CD40 and TLR agonists as a cancer vaccine.

Sat Mar 15 00:00:00 EDT 2008

Modulation of pulmonary dendritic cell function during mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Liu Y,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Fri Feb 01 00:00:00 EST 2008

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 16-7021-85) in ELISA

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

Gene Aliases: Il-2; Il2

UniProt ID: (Mouse) P04351

Entrez Gene ID: (Mouse) 16183

Molecular Function: actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Function(s)

Process(es)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

IL-2 Monoclonal Antibody (JES6-5H4), Functional Grade, eBioscience™

Product Details

16-7021-85

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (FN)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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