Invitrogen

IL-2 Monoclonal Antibody (JES6-5H4), APC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17 Published Figures

33 References

View all (105) IL-2 antibodies

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IL-2 Antibody in Flow Cytometry (Flow) — Mouse splenocytes were stimulated with PMA/Ionomycin in the presence of Monensin, then surface stained with Anti-Mouse CD4 PE (Product # 12-0041-82), followed by intracellular staining with with 0.125 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse IL-2 APC (right). Total viable cells were used for analysis.

IL-2 Antibody — Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IL-2 clone JES6-5H4 stains a large subset of CD4+ T cells and a minor subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IL-2 (clone JES6-5H4). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. {TM}

Product Details

17-7021-82

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.25 µg/test	View 32 publications 32 publications
ELISA (ELISA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2b, kappa
Recommended Isotype Control	Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	JES6-5H4
Conjugate	APC Alexa Fluor 488 APC-eFluor 780 Biotin Brilliant UV 737 eFluor 450 FITC Functional Grade PE PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Unconjugated
Excitation/Emission Max	651/660 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_469490

Product Specific Information

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported: JES6-5H4 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This JES6-5H4 antibody has been tested intracellular staininf of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Lysosomal acid lipase, CSF1R, and PD-L1 determine functions of CD11c+ myeloid-derived suppressor cells.

JCI insight

Zhao T,Liu S,Ding X,Johnson EM,Hanna NH,Singh K,Sen CK,Wan J,Du H,Yan C

17-7021-82 was used in Flow cytometry/Cell sorting to investigate if CD11c+ cells switched their functions to immune suppression and tumor growth stimulation through CSF1R/PD-L1 upregulation and metabolic reprogramming.

Thu Sep 08 00:00:00 EDT 2022

A Methyltransferase-Defective Vesicular Stomatitis Virus-Based SARS-CoV-2 Vaccine Candidate Provides Complete Protection against SARS-CoV-2 Infection in Hamsters.

Journal of virology

Lu M,Zhang Y,Dravid P,Li A,Zeng C,Kc M,Trivedi S,Sharma H,Chaiwatpongsakorn S,Zani A,Kenney A,Cai C,Ye C,Liang X,Qiu J,Martinez-Sobrido L,Yount JS,Boyaka PN,Liu SL,Peeples ME,Kapoor A,Li J

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Rescuing ESAT-6 Specific CD4 T Cells From Terminal Differentiation Is Critical for Long-Term Control of Murine Mtb Infection.

Frontiers in immunology

Clemmensen HS,Knudsen NPH,Billeskov R,Rosenkrands I,Jungersen G,Aagaard C,Andersen P,Mortensen R

17-7021-81 was used in Flow Cytometry to show long term control of murine mycobacterium tuberculosis infection is facilitated by rescue of ESAT-6 specific CD4 T cells.

Mon Jun 14 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

17-7021-82 was used in Flow Cytometry to investigate whether murine Granulocyte-macrophage colony-stimulating factor (GM-CSF) represents a suitable adjuvant for SARS-CoV-2 immunization.

Mon May 10 00:00:00 EDT 2021

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Modulation of Vaccine-Induced HIV-1-Specific Immune Responses by Co-Electroporation of PD-L1 Encoding DNA.

Vaccines

Tannig P,Peter AS,Lapuente D,Klessing S,Damm D,Tenbusch M,Überla K,Temchura V

17-7021-82 was used in Flow Cytometry to indicate that co-electroporation of soluble checkpoint ectodomains together with DNA-based vaccines has modulatory effects on vaccine-induced immune responses that could improve vaccine efficacies.

Tue Jan 14 00:00:00 EST 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.

Cell reports

Gagnon JD,Kageyama R,Shehata HM,Fassett MS,Mar DJ,Wigton EJ,Johansson K,Litterman AJ,Odorizzi P,Simeonov D,Laidlaw BJ,Panduro M,Patel S,Jeker LT,Feeney ME,McManus MT,Marson A,Matloubian M,Sanjabi S,Ansel KM

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Tue Aug 20 00:00:00 EDT 2019

Molecular architecture and regulation of BCL10-MALT1 filaments.

Nature communications

Schlauderer F,Seeholzer T,Desfosses A,Gehring T,Strauss M,Hopfner KP,Gutsche I,Krappmann D,Lammens K

17-7021 was used in Flow cytometry/Cell sorting to develop a model for the assembly and architecture of the CARD11-BCL10-MALT1 signalling complex and how it functions as a signalling hub in T-lymphocytes.

Tue Oct 02 00:00:00 EDT 2018

Mannose receptor high, M2 dermal macrophages mediate nonhealing Leishmania major infection in a Th1 immune environment.

The Journal of experimental medicine

Lee SH,Charmoy M,Romano A,Paun A,Chaves MM,Cope FO,Ralph DA,Sacks DL

17-7021 was used in Flow cytometry/Cell sorting to study the origin and functional specialisation of dermal macrophages in cutaneous infections.

Tue Jan 02 00:00:00 EST 2018

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

NFATc1 controls the cytotoxicity of CD8⁺ T cells.

Nature communications

Klein-Hessling S,Muhammad K,Klein M,Pusch T,Rudolf R,Flöter J,Qureischi M,Beilhack A,Vaeth M,Kummerow C,Backes C,Schoppmeyer R,Hahn U,Hoth M,Bopp T,Berberich-Siebelt F,Patra A,Avots A,Müller N,Schulze A,Serfling E

17-7021 was used in Flow cytometry/Cell sorting to indicate that NFATc1 is an important regulator of cytotoxic T lymphocyte effector functions.

Mon Sep 11 00:00:00 EDT 2017

Vaccination with High-Affinity Epitopes Impairs Antitumor Efficacy by Increasing PD-1 Expression on CD8⁺ T Cells.

Cancer immunology research

Zahm CD,Colluru VT,McNeel DG

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Mycobacterium tuberculosis multistage antigens confer comprehensive protection against pre- and post-exposure infections by driving Th1-type T cell immunity.

Oncotarget

Ma J,Tian M,Fan X,Yu Q,Jing Y,Wang W,Li L,Zhou Z

17-7021 was used in Flow cytometry/Cell sorting to suggest that multistage-specific antigens could be targets for the next generation TB vaccine.

Tue Sep 27 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

17-7021 was used in Flow cytometry/Cell sorting to indicate a close link between NFATc1 and IL-10 expression in B cells, suggesting that NFATc1 may be a potential target to treat human psoriasis.

Wed May 25 00:00:00 EDT 2016

Inadequate activation of the HBsAg-specific Th cells by APCs leads to hyporesponsiveness to HBsAg vaccine in B10.S mice.

Human vaccines & immunotherapeutics

Li X,Xu J,Lv Z,Wang J,Sun S,Zhu W,Wang B,He R,Qu D

17-7021 was used in Flow cytometry/Cell sorting to study how the activities of APCs were significantly lower in mice immunised with HBsAg vaccine, resulting in an inferior activation of HBsAg-specific Th cells.

Tue Apr 19 00:00:00 EDT 2016

Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.

Nature communications

Meininger I,Griesbach RA,Hu D,Gehring T,Seeholzer T,Bertossi A,Kranich J,Oeckinghaus A,Eitelhuber AC,Greczmiel U,Gewies A,Schmidt-Supprian M,Ruland J,Brocker T,Heissmeyer V,Heyd F,Krappmann D

17-7021 was used in Flow cytometry/Cell sorting to study how TCR-induced alternative splicing augments MALT1 scaffolding to enhance downstream signalling and to promote optimal T-cell activation.

Tue Apr 12 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using IL-2 monoclonal antibody (Product # 17-7021-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Protection against Mycobacterium tuberculosis infection offered by a new multistage subunit vaccine correlates with increased number of IFN-γ+ IL-2+ CD4+ and IFN-γ+ CD8+ T cells.

PloS one

Wang X,Zhang J,Liang J,Zhang Y,Teng X,Yuan X,Fan X

17-7021 was used in Flow cytometry/Cell sorting to investigate protein subunit vaccinations for control of tuberculosis, showing that a new multistage subunit vaccine increases numbers of T cells and protects against TB.

Wed Mar 09 00:00:00 EST 2016

High-frequency vaccine-induced CD8⁺ T cells specific for an epitope naturally processed during infection with Mycobacterium tuberculosis do not confer protection.

European journal of immunology

Lindenstrøm T,Aagaard C,Christensen D,Agger EM,Andersen P

17-7021 was used in Flow cytometry/Cell sorting to study the role of CD8 T cells in resistance to mycobacterial infections.

Sun Jun 01 00:00:00 EDT 2014

The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis.

Nature communications

Kuwahara M,Suzuki J,Tofukuji S,Yamada T,Kanoh M,Matsumoto A,Maruyama S,Kometani K,Kurosaki T,Ohara O,Nakayama T,Yamashita M

17-7021 was used in Flow cytometry/Cell sorting to show that T cell-specific Menin deficiency results in the premature senescence of CD4 T cells, which is accompanied by the senescence-associated secretory phenotype after antigenic stimulation and dysregulated cytokine production.

Wed Apr 02 00:00:00 EDT 2014

IL-21 restricts virus-driven Treg cell expansion in chronic LCMV infection.

PLoS pathogens

Schmitz I,Schneider C,Fröhlich A,Frebel H,Christ D,Leonard WJ,Sparwasser T,Oxenius A,Freigang S,Kopf M

17-7021 was used in Flow cytometry/Cell sorting to investigate the role of Foxp3+ regulatory T (Treg) cells during viral infections, showing that IL-21 restricts virus-driven Treg cell expansion in chronic LCMV infection.

Mon Dec 09 00:00:00 EST 2013

Epstein-Barr virus latent membrane protein 2A exacerbates experimental autoimmune encephalomyelitis and enhances antigen presentation function.

Scientific reports

Chang RA,Miller SD,Longnecker R

17-7021 was used in an ELISpot assay to investigate the Epstein-Barr virus as a possible etiological agent involved in multiple sclerosis.

Mon Feb 25 00:00:00 EST 2013

Helios induces epigenetic silencing of IL2 gene expression in regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Baine I,Basu S,Ames R,Sellers RS,Macian F

17-7021 was used in Flow cytometry/Cell sorting to identify that the transcription factor, Helios, maintains the anergic phenotype of Tregs by suppressing transcription of interleukin 2.

Fri Feb 01 00:00:00 EST 2013

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

17-7021 was used in Flow cytometry/Cell sorting to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

The Thai phase III trial (RV144) vaccine regimen induces T cell responses that preferentially target epitopes within the V2 region of HIV-1 envelope.

Journal of immunology (Baltimore, Md. : 1950)

de Souza MS,Ratto-Kim S,Chuenarom W,Schuetz A,Chantakulkij S,Nuntapinit B,Valencia-Micolta A,Thelian D,Nitayaphan S,Pitisuttithum P,Paris RM,Kaewkungwal J,Michael NL,Rerks-Ngarm S,Mathieson B,Marovich M,Currier JR,Kim JH

17-7021 was used in Flow cytometry/Cell sorting to reveal T cell response of HIV-1 vaccine recipients was directed to a particular region of viral envelope.

Tue May 15 00:00:00 EDT 2012

CD4+ T cells modulate expansion and survival but not functional properties of effector and memory CD8+ T cells induced by malaria sporozoites.

PloS one

Overstreet MG,Chen YC,Cockburn IA,Tse SW,Zavala F

17-7021 was used in Flow cytometry/Cell sorting to investigate CD4+ T cells modulation of effector and memory CD8+ T cells, induced by malaria sporozoites.

Tue Jan 04 00:00:00 EST 2011

IL-21 deficiency influences CD8 T cell quality and recall responses following an acute viral infection.

Journal of immunology (Baltimore, Md. : 1950)

Yi JS,Ingram JT,Zajac AJ

17-7021 was used in Flow cytometry/Cell sorting to investigate the importance of IL-21 in determining CD8 T cell function, quality and maintenance following lymphocytic choriomeningitis virus infection in mice.

Fri Oct 15 00:00:00 EDT 2010

Dendritic cell and NK cell reciprocal cross talk promotes gamma interferon-dependent immunity to blood-stage Plasmodium chabaudi AS infection in mice.

Infection and immunity

Ing R,Stevenson MM

17-7021 was used in Immunofluorescence to elucidate the signalling mechanisms underlying interferon-γ-dependent immunity to malaria.

Sun Feb 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

Gene Aliases: Il-2; Il2

UniProt ID: (Mouse) P04351

Entrez Gene ID: (Mouse) 16183

Molecular Function: actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Function(s)

Process(es)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

IL-2 Monoclonal Antibody (JES6-5H4), APC, eBioscience™

Product Details

17-7021-82

Flow Cytometry (Flow)

ELISA (ELISA)

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Published species

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Recommended Isotype Control

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