Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

16 References

View all (90) IL-2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IL-2 Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0049-42) and 0.125 µg IL-2 Monoclonal Antibody followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Cells in the lymphocyte gate were used for analysis.

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

14-7029-81

Applications	Tested Dilution	Publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 2 publications 2 publications
Flow Cytometry (Flow)	Assay-Dependent	View 11 publications 11 publications
ELISA (ELISA)	1-4 µg/mL	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human
Published species	Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey
Host/Isotype	Rat / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	MQ1-17H12
Conjugate	Unconjugated Alexa Fluor 532 APC eFluor 450 FITC PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_468408

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: The MQ1-17H12 antibody has been reported for use as capture antibody for ELISA and for intracellular staining for flow cytometric analysis. Fluorochrome conjugated MQ1-17H12 antibody is recommended for use in intracellular staining for flow cytometry.

Applications Tested: The MQ1-17H12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-2 (IL-2) in combination with the biotin anti-human IL-2 poly (13-7028) antibody for detection and recombinant human IL-2 (14-8029) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Immunity

Schwabenland M,Salié H,Tanevski J,Killmer S,Lago MS,Schlaak AE,Mayer L,Matschke J,Püschel K,Fitzek A,Ondruschka B,Mei HE,Boettler T,Neumann-Haefelin C,Hofmann M,Breithaupt A,Genc N,Stadelmann C,Saez-Rodriguez J,Bronsert P,Knobeloch KP,Blank T,Thimme R,Glatzel M,Prinz M,Bengsch B

14-7029-81 was used in Mass cytometry to identify profound neuroinflammation with activation of innate and adaptive immune cells as correlates of COVID-19 neuropathology, with implications for potential therapeutic strategies.

Tue Jul 13 00:00:00 EDT 2021

A novel format for recombinant antibody-interleukin-2 fusion proteins exhibits superior tumor-targeting properties in vivo.

Oncotarget

Ongaro T,Gouyou B,Stringhini M,Corbellari R,Neri D,Villa A

14-7029-85 was used in Immunohistochemistry (Frozen) to study the in vivo tumor-homing and anti-cancer activity of antibody-interleukin 2 fusion proteins.

Tue Oct 13 00:00:00 EDT 2020

A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas.

Frontiers in oncology

De Luca R,Gouyou B,Ongaro T,Villa A,Ziffels B,Sannino A,Buttinoni G,Galeazzi S,Mazzacuva M,Neri D

14-7029-85 was used in Immunohistochemistry (Frozen), Flow Cytometry to report the cloning, expression and characterization of IL2-XE114-TNFmut, a dual-cytokine biopharmaceutical featuring the sequential fusion of interleukin-2 (IL2) with the XE114 antibody in scFv format and a tumor necrosis factor mutant (TNFmut).

Thu Oct 01 00:00:00 EDT 2020

A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas.

Frontiers in oncology

De Luca R,Gouyou B,Ongaro T,Villa A,Ziffels B,Sannino A,Buttinoni G,Galeazzi S,Mazzacuva M,Neri D

Thu Oct 01 00:00:00 EDT 2020

TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma.

Cancer immunology research

Josefsson SE,Beiske K,Blaker YN,Førsund MS,Holte H,Østenstad B,Kimby E,Köksal H,Wälchli S,Bai B,Smeland EB,Levy R,Kolstad A,Huse K,Myklebust JH

14-7029 was used in Flow cytometry/Cell sorting to show that TIGIT and PD-1 mark dysfunctional T cells and suggest that TIGIT and PD-1 coblockade should be further explored to elicit potent antitumor responses in patients with NHL.

Fri Mar 01 00:00:00 EST 2019

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Prostate Cancer Cells Express More Androgen Receptor (AR) Following Androgen Deprivation, Improving Recognition by AR-Specific T Cells.

Cancer immunology research

Olson BM,Gamat M,Seliski J,Sawicki T,Jeffery J,Ellis L,Drake CG,Weichert J,McNeel DG

14-7029 was used in Flow cytometry/Cell sorting to suggest that ADT combined with AR-directed immunotherapy targets a major mechanism of resistance, overexpression of the AR.

Fri Dec 01 00:00:00 EST 2017

Optimization of mass cytometry sample cryopreservation after staining.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Sumatoh HR,Teng KW,Cheng Y,Newell EW

14702981 was used in flow cytometry to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment

Sun Jan 01 00:00:00 EST 2017

Loss of immune tolerance to IL-2 in type 1 diabetes.

Nature communications

Pérol L,Lindner JM,Caudana P,Nunez NG,Baeyens A,Valle A,Sedlik C,Loirat D,Boyer O,Créange A,Cohen JL,Rogner UC,Yamanouchi J,Marchant M,Leber XC,Scharenberg M,Gagnerault MC,Mallone R,Battaglia M,Santamaria P,Hartemann A,Traggiai E,Piaggio E

14-7029 was used in an ELISA assay to study the role of the loss of immune tolerance to IL-2 in type 1 diabetes.

Thu Oct 06 00:00:00 EDT 2016

Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.

American journal of reproductive immunology (New York, N.Y. : 1989)

Shanmugasundaram U,Hilton JF,Critchfield JW,Greenblatt RM,Giudice LC,Averbach S,Seidman D,Shacklett BL,Smith-McCune K

14-7029 was used in Flow cytometry/Cell sorting to study the impact of the levonorgestrel-releasing intrauterine device on immune parameters of the upper female reproductive tract.

Mon Aug 01 00:00:00 EDT 2016

PD-1 marks dysfunctional regulatory T cells in malignant gliomas.

JCI insight

Lowther DE,Goods BA,Lucca LE,Lerner BA,Raddassi K,van Dijk D,Hernandez AL,Duan X,Gunel M,Coric V,Krishnaswamy S,Love JC,Hafler DA

14-7029 was used in Flow cytometry/Cell sorting to study how high PD-1 expression on human Tregs identifies dysfunctional, exhausted Tregs secreting IFN-γ that exist in healthy individuals and are enriched in tumor infiltrates.

Thu Apr 21 00:00:00 EDT 2016

Fiber composite slices for multiplexed immunoassays.

Biomicrofluidics

Kim J,Bae S,Song S,Chung K,Kwon S

14-7029 was used in Immuno-assay to describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads.

Wed Jul 01 00:00:00 EDT 2015

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-7029 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

14702981 was used in flow cytometry to investigate the impact of CCR6(+)CXCR3(+)CCR4(-) cells in latent tuberculosis infection

Mon Sep 15 00:00:00 EDT 2014

A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251.

Journal of virology

Van Rompay KK,Hunter Z,Jayashankar K,Peabody J,Montefiori D,LaBranche CC,Keele BF,Jensen K,Abel K,Chackerian B

14-7029 was used in Flow cytometry/Cell sorting to investigate whether prophylactic immunisation of rhesus macaques with a vaccine against CCR5 could prevent or suppress SIV.

Sat Feb 01 00:00:00 EST 2014

CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.

AIDS research and human retroviruses

Lin PL,Rutledge T,Green AM,Bigbee M,Fuhrman C,Klein E,Flynn JL

14-7029 was used in Flow cytometry/Cell sorting to study initial CD4 T cell protection against acute M.tuberculosis infection and reactivation upon latent infection.

Sat Dec 01 00:00:00 EST 2012

CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.

AIDS research and human retroviruses

Lin PL,Rutledge T,Green AM,Bigbee M,Fuhrman C,Klein E,Flynn JL

14-7029 was used in Flow cytometry/Cell sorting to study initial CD4 T cell protection against acute M.tuberculosis infection and reactivation upon latent infection.

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

Gene Aliases: IL-2; IL2; lymphokine; TCGF

UniProt ID: (Human) P60568

Entrez Gene ID: (Human) 3558

Function(s)