Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

24 References

View all (119) IL-2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IL-2 Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0049-42) and 0.125 µg IL-2 Monoclonal Antibody followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Cells in the lymphocyte gate were used for analysis.

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis.

Product Details

14-7029-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 17 publications 17 publications

ELISA (ELISA)

1-4 µg/mL

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_468408

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: The MQ1-17H12 antibody has been reported for use as capture antibody for ELISA and for intracellular staining for flow cytometric analysis. Fluorochrome conjugated MQ1-17H12 antibody is recommended for use in intracellular staining for flow cytometry.

Applications Tested: The MQ1-17H12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-2 (IL-2) in combination with the biotin anti-human IL-2 poly (13-7028) antibody for detection and recombinant human IL-2 (14-8029) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs.

Nature cell biology

Truong DJ,Armbrust N,Geilenkeuser J,Lederer EM,Santl TH,Beyer M,Ittermann S,Steinmaßl E,Dyka M,Raffl G,Phlairaharn T,Greisle T,Živanić M,Grosch M,Drukker M,Westmeyer GG

14-7029-81 was used in Enzyme-linked immunosorbent assay to develop the intron-encoded scarless programmable extranuclear cistronic transcript (INSPECT) as a minimally invasive transcriptional reporter embedded within an intron of a gene of interest.

Tue Nov 01 00:00:00 EDT 2022

Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.

Nature communications

Koerber N,Priller A,Yazici S,Bauer T,Cheng CC,Mijočević H,Wintersteller H,Jeske S,Vogel E,Feuerherd M,Tinnefeld K,Winter C,Ruland J,Gerhard M,Haller B,Christa C,Zelger O,Roggendorf H,Halle M,Erber J,Lingor P,Keppler O,Zehn D,Protzer U,Knolle PA

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Mon Jan 10 00:00:00 EST 2022

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Thu Nov 25 00:00:00 EST 2021

Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.

Journal of thrombosis and haemostasis : JTH

Asgari A,Lesyk G,Poitras E,Govindasamy N,Terry K,To R,Back V,Rudzinski JK,Lewis JD,Jurasz P

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.

Nature communications

Alrubayyi A,Gea-Mallorquí E,Touizer E,Hameiri-Bowen D,Kopycinski J,Charlton B,Fisher-Pearson N,Muir L,Rosa A,Roustan C,Earl C,Cherepanov P,Pellegrino P,Waters L,Burns F,Kinloch S,Dong T,Dorrell L,Rowland-Jones S,McCoy LE,Peppa D

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Immunity

Schwabenland M,Salié H,Tanevski J,Killmer S,Lago MS,Schlaak AE,Mayer L,Matschke J,Püschel K,Fitzek A,Ondruschka B,Mei HE,Boettler T,Neumann-Haefelin C,Hofmann M,Breithaupt A,Genc N,Stadelmann C,Saez-Rodriguez J,Bronsert P,Knobeloch KP,Blank T,Thimme R,Glatzel M,Prinz M,Bengsch B

14-7029-81 was used in Mass cytometry to identify profound neuroinflammation with activation of innate and adaptive immune cells as correlates of COVID-19 neuropathology, with implications for potential therapeutic strategies.

Tue Jul 13 00:00:00 EDT 2021

A novel format for recombinant antibody-interleukin-2 fusion proteins exhibits superior tumor-targeting properties in vivo.

Oncotarget

Ongaro T,Gouyou B,Stringhini M,Corbellari R,Neri D,Villa A

14-7029-85 was used in Immunohistochemistry (Frozen) to study the in vivo tumor-homing and anti-cancer activity of antibody-interleukin 2 fusion proteins.

Tue Oct 13 00:00:00 EDT 2020

A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas.

Frontiers in oncology

De Luca R,Gouyou B,Ongaro T,Villa A,Ziffels B,Sannino A,Buttinoni G,Galeazzi S,Mazzacuva M,Neri D

14-7029-85 was used in Immunohistochemistry (Frozen), Flow Cytometry to report the cloning, expression and characterization of IL2-XE114-TNFmut, a dual-cytokine biopharmaceutical featuring the sequential fusion of interleukin-2 (IL2) with the XE114 antibody in scFv format and a tumor necrosis factor mutant (TNFmut).

Thu Oct 01 00:00:00 EDT 2020

A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas.

Frontiers in oncology

De Luca R,Gouyou B,Ongaro T,Villa A,Ziffels B,Sannino A,Buttinoni G,Galeazzi S,Mazzacuva M,Neri D

Thu Oct 01 00:00:00 EDT 2020

Antibody-Based Delivery of Cytokine Payloads to Carbonic Anhydrase IX Leads to Cancer Cures in Immunocompetent Tumor-Bearing Mice.

Molecular cancer therapeutics

Ziffels B,Stringhini M,Probst P,Fugmann T,Sturm T,Neri D

14-7029-81 was used in Immunohistochemistry-immunofluorescence to describe the design, production, and characterization of four novel antibody-cytokine fusion proteins directed against human carbonic anhydrase IX, a highly validated marker of hypoxia that is overexpressed in clear cell renal cell carcinoma and other malignancies.

Sun Sep 01 00:00:00 EDT 2019

TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma.

Cancer immunology research

Josefsson SE,Beiske K,Blaker YN,Førsund MS,Holte H,Østenstad B,Kimby E,Köksal H,Wälchli S,Bai B,Smeland EB,Levy R,Kolstad A,Huse K,Myklebust JH

14-7029 was used in Flow cytometry/Cell sorting to show that TIGIT and PD-1 mark dysfunctional T cells and suggest that TIGIT and PD-1 coblockade should be further explored to elicit potent antitumor responses in patients with NHL.

Fri Mar 01 00:00:00 EST 2019

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

14-7029 was used in Flow cytometry/Cell sorting to develop a novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Tue May 01 00:00:00 EDT 2018

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

Published figure using IL-2 monoclonal antibody (Product # 14-7029-81) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

Prostate Cancer Cells Express More Androgen Receptor (AR) Following Androgen Deprivation, Improving Recognition by AR-Specific T Cells.

Cancer immunology research

Olson BM,Gamat M,Seliski J,Sawicki T,Jeffery J,Ellis L,Drake CG,Weichert J,McNeel DG

14-7029 was used in Flow cytometry/Cell sorting to suggest that ADT combined with AR-directed immunotherapy targets a major mechanism of resistance, overexpression of the AR.

Fri Dec 01 00:00:00 EST 2017

Optimization of mass cytometry sample cryopreservation after staining.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Sumatoh HR,Teng KW,Cheng Y,Newell EW

14702981 was used in flow cytometry to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment

Sun Jan 01 00:00:00 EST 2017

Loss of immune tolerance to IL-2 in type 1 diabetes.

Nature communications

Pérol L,Lindner JM,Caudana P,Nunez NG,Baeyens A,Valle A,Sedlik C,Loirat D,Boyer O,Créange A,Cohen JL,Rogner UC,Yamanouchi J,Marchant M,Leber XC,Scharenberg M,Gagnerault MC,Mallone R,Battaglia M,Santamaria P,Hartemann A,Traggiai E,Piaggio E

14-7029 was used in an ELISA assay to study the role of the loss of immune tolerance to IL-2 in type 1 diabetes.

Thu Oct 06 00:00:00 EDT 2016

Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.

American journal of reproductive immunology (New York, N.Y. : 1989)

Shanmugasundaram U,Hilton JF,Critchfield JW,Greenblatt RM,Giudice LC,Averbach S,Seidman D,Shacklett BL,Smith-McCune K

14-7029 was used in Flow cytometry/Cell sorting to study the impact of the levonorgestrel-releasing intrauterine device on immune parameters of the upper female reproductive tract.

Mon Aug 01 00:00:00 EDT 2016

PD-1 marks dysfunctional regulatory T cells in malignant gliomas.

JCI insight

Lowther DE,Goods BA,Lucca LE,Lerner BA,Raddassi K,van Dijk D,Hernandez AL,Duan X,Gunel M,Coric V,Krishnaswamy S,Love JC,Hafler DA

14-7029 was used in Flow cytometry/Cell sorting to study how high PD-1 expression on human Tregs identifies dysfunctional, exhausted Tregs secreting IFN-γ that exist in healthy individuals and are enriched in tumor infiltrates.

Thu Apr 21 00:00:00 EDT 2016

Fiber composite slices for multiplexed immunoassays.

Biomicrofluidics

Kim J,Bae S,Song S,Chung K,Kwon S

14-7029 was used in Immuno-assay to describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads.

Wed Jul 01 00:00:00 EDT 2015

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-7029 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

14702981 was used in flow cytometry to investigate the impact of CCR6(+)CXCR3(+)CCR4(-) cells in latent tuberculosis infection

Mon Sep 15 00:00:00 EDT 2014

A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251.

Journal of virology

Van Rompay KK,Hunter Z,Jayashankar K,Peabody J,Montefiori D,LaBranche CC,Keele BF,Jensen K,Abel K,Chackerian B

14-7029 was used in Flow cytometry/Cell sorting to investigate whether prophylactic immunisation of rhesus macaques with a vaccine against CCR5 could prevent or suppress SIV.

Sat Feb 01 00:00:00 EST 2014

CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.

AIDS research and human retroviruses

Lin PL,Rutledge T,Green AM,Bigbee M,Fuhrman C,Klein E,Flynn JL

14-7029 was used in Flow cytometry/Cell sorting to study initial CD4 T cell protection against acute M.tuberculosis infection and reactivation upon latent infection.

Sat Dec 01 00:00:00 EST 2012

CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.

AIDS research and human retroviruses

Lin PL,Rutledge T,Green AM,Bigbee M,Fuhrman C,Klein E,Flynn JL

14-7029 was used in Flow cytometry/Cell sorting to study initial CD4 T cell protection against acute M.tuberculosis infection and reactivation upon latent infection.

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

Gene Aliases: IL-2; IL2; lymphokine; TCGF

UniProt ID: (Human) P60568

Entrez Gene ID: (Human) 3558

Function(s)

Process(es)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™

Product Details

14-7029-81

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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