Invitrogen

IL-22 Monoclonal Antibody (22URTI), PE-Cyanine7, eBioscience™

Product Image Gallery

IL-22 Antibody in Flow Cytometry (Flow) — Intracellular staining of CD4-enriched Th17-polarized (using Human IL-23 Recombinant Protein (Product # 14-8239-63), normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-22 PE-Cyanine7. Cultures were treated with Protein Transport Inhibitor Cocktail alone (Product # 00-4980-03) (left) or Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours prior to intracellular staining using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Cells in the lymphocyte gate were used for analysis.

Product Details

25-7229-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.06 µg)/test	View 19 publications 19 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	22URTI
Conjugate	PE-Cyanine7 Brilliant UV 737 eFluor 450 eFluor 660 FITC PE PerCP-eFluor 710
Excitation/Emission Max	569/780 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10853346

Product Specific Information

Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22.

Applications Reported: This 22URTI antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral CD4 cells cultured in Th17 polarizing conditions. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Thu Nov 25 00:00:00 EST 2021

CD4 T-Cell Dysregulation in Psoriatic Arthritis Reveals a Regulatory Role for IL-22.

Frontiers in immunology

Ezeonyeji A,Baldwin H,Vukmanovic-Stejic M,Ehrenstein MR

25-7229 was used in Flow cytometry/Cell sorting to examine the role of IL-22 in patients with psoriatic arthritis (PsA).

Sat Jan 09 00:00:00 EST 2021

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

25-7229 was used in Flow cytometry/Cell sorting to perform a longitudinal assessment of individuals recovered from mild COVID-19 to determine whether they develop and sustain multifaceted SARS-CoV-2-specific immunological memory.

Thu Jan 07 00:00:00 EST 2021

HIV Skews a Balanced Mtb-Specific Th17 Response in Latent Tuberculosis Subjects to a Pro-inflammatory Profile Independent of Viral Load.

Cell reports

Rakshit S,Hingankar N,Alampalli SV,Adiga V,Sundararaj BK,Sahoo PN,Finak G,Uday Kumar J AJ,Dhar C,D'Souza G,Virkar RG,Ghate M,Thakar MR,Paranjape RS,De Rosa SC,Ottenhoff THM,Vyakarnam A

25-7229 was used in Flow cytometry/Cell sorting to propose that beyond HIV-induced loss of Mtb-specific CD4+ T cells, the associated dysregulation of Mtb-specific T cell homeostasis can potentially enhance the onset of TB in LTBI subjects.

Tue Dec 01 00:00:00 EST 2020

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Oncology reports

Zhu F,Qiu T,Zhu S,Zhao K,Chen C,Qiao J,Pan B,Yan Z,Chen W,Liu Q,Wu Q,Cao J,Sang W,Zeng L,Sun H,Li Z,Xu K

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Human CD5⁺ Innate Lymphoid Cells Are Functionally Immature and Their Development from CD34⁺ Progenitor Cells Is Regulated by Id2.

Frontiers in immunology

Nagasawa M,Germar K,Blom B,Spits H

25-7229 was used in Flow cytometry/Cell sorting to show the development of human innate lymphoid cells to immature CD5+ innate lymphoid cells is regulated by DNA-binding protein 2.

Wed Nov 20 00:00:00 EST 2019

Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces T_H2/T_H17 polarization through dectin-2.

The Journal of allergy and clinical immunology

Liang Y,Yu B,Chen J,Wu H,Xu Y,Yang B,Lu Q

25-7229 was used in Flow cytometry/Cell sorting to investigate epigenetic mechanisms underlying FCER1G demethylation and upregulation of FcRγ-related receptors on APCs and the consequent effect on allergic responses.

Tue Oct 01 00:00:00 EDT 2019

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue.

Scientific reports

Caër C,Rouault C,Le Roy T,Poitou C,Aron-Wisnewsky J,Torcivia A,Bichet JC,Clément K,Guerre-Millo M,André S

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Wed Jun 07 00:00:00 EDT 2017

Antigen-Presenting Human γδ T Cells Promote Intestinal CD4⁺ T Cell Expression of IL-22 and Mucosal Release of Calprotectin.

Journal of immunology (Baltimore, Md. : 1950)

Tyler CJ,McCarthy NE,Lindsay JO,Stagg AJ,Moser B,Eberl M

25-7229 was used in Flow cytometry/Cell sorting to study the role of γδ T cells in the regulation of IL-22 expression.

Mon May 01 00:00:00 EDT 2017

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

25-7229 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 EST 2017

Downregulation of RUNX3 moderates the frequency of Th17 and Th22 cells in patients with psoriasis.

Molecular medicine reports

Fu D,Song X,Hu H,Sun M,Li Z,Tian Z

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.

Gut

Mann ER,Bernardo D,English NR,Landy J,Al-Hassi HO,Peake ST,Man R,Elliott TR,Spranger H,Lee GH,Parian A,Brant SR,Lazarev M,Hart AL,Li X,Knight SC

25-7229 was used in Flow cytometry/Cell sorting to investigate and distinguish properties of dendritic cells in the colon and the ileum.

Mon Feb 01 00:00:00 EST 2016

Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.

Stem cell reports

Wang WB,Yen ML,Liu KJ,Hsu PJ,Lin MH,Chen PM,Sudhir PR,Chen CH,Chen CH,Sytwu HK,Yen BL

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Jejunal T Cell Inflammation in Human Obesity Correlates with Decreased Enterocyte Insulin Signaling.

Cell metabolism

Monteiro-Sepulveda M,Touch S,Mendes-Sá C,André S,Poitou C,Allatif O,Cotillard A,Fohrer-Ting H,Hubert EL,Remark R,Genser L,Tordjman J,Garbin K,Osinski C,Sautès-Fridman C,Leturque A,Clément K,Brot-Laroche E

25-7229 was used in Flow cytometry/Cell sorting to study inflammation of the absorptive small intestine in obese and diabetic patients.

Tue Jul 07 00:00:00 EDT 2015

Early initiation of combined antiretroviral therapy preserves immune function in the gut of HIV-infected patients.

Mucosal immunology

Kök A,Hocqueloux L,Hocini H,Carrière M,Lefrou L,Guguin A,Tisserand P,Bonnabau H,Avettand-Fenoel V,Prazuck T,Katsahian S,Gaulard P,Thiébaut R,Lévy Y,Hüe S

25-7229 was used in Flow cytometry/Cell sorting to investigate determinants of gut mucosal restoration during antiretroviral treatment for HIV-1 infection.

Thu Jan 01 00:00:00 EST 2015

IL32 is progressively expressed in mycosis fungoides independent of helper T-cell 2 and helper T-cell 9 polarization.

Cancer immunology research

Ohmatsu H,Humme D,Gulati N,Gonzalez J,Möbs M,Suárez-Fariñas M,Cardinale I,Mitsui H,Guttman-Yassky E,Sterry W,Krueger JG

25-7229 was used in Flow cytometry/Cell sorting to reveal a potential, unique role for IL32 in mycosis fungoides progression as an autocrine cytokine.

Mon Sep 01 00:00:00 EDT 2014

T cell-derived IL-22 amplifies IL-1β-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.

Diabetes

Dalmas E,Venteclef N,Caer C,Poitou C,Cremer I,Aron-Wisnewsky J,Lacroix-Desmazes S,Bayry J,Kaveri SV,Clément K,André S,Guerre-Millo M

25-7229 was used in Flow cytometry/Cell sorting to investigate the contribution of different cytokines to the proinflammatory signature of adipose tissue macrophages in type 2 diabetic obese patients.

Sun Jun 01 00:00:00 EDT 2014

Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.

PloS one

Zhang L,Li YG,Li YH,Qi L,Liu XG,Yuan CZ,Hu NW,Ma DX,Li ZF,Yang Q,Li W,Li JM

Published figure using IL-22 monoclonal antibody (Product # 25-7229-42) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Cytokine Zcyto18; il 22; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor; IL-22; IL-TIF; ILN; Interleukin; Interleukin-22; Interleukin22

Gene Aliases: IL-21; IL-22; IL-D110; IL-TIF; IL22; ILTIF; TIFa; TIFIL-23; UNQ3099/PRO10096; ZCYTO18

UniProt ID: (Human) Q9GZX6

Entrez Gene ID: (Human) 50616

Function(s)

acute-phase response inflammatory response immune response cell-cell signaling positive regulation of JAK-STAT cascade response to glucocorticoid

Process(es)

cytokine activity protein binding interleukin-22 receptor binding

IL-22 Monoclonal Antibody (22URTI), PE-Cyanine7, eBioscience™

Product Details

25-7229-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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