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Western blot of IL-25/IL-17E in human prostate lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with an IL-25/IL-17E polyclonal antibody (Product# PA5-37968) at a dilution of 0.5ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence HGEKGTHKGYCLER, from the internal region of IL25|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
As a member of the IL-17 family, IL-25 has been identified as a pro-inflammatory cytokine which is produced by Th17 effector cells thus enabling the TH17 subset to initiate or maintain the autoimmune process in diseases such as Rheumatoid Arthritis. IL-25 appears to stimulate IL-8 production and itself appears to be activated through IL-23, IL-6, and TGF-beta. Studies of IL-25 expression and activation through NF-kB taken together with IL-17 production and Th17 polarization through TLR ligand activation further closely tie the IL-17 and Th17 family together with the TLR signaling network initiating and controlling immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IL17E; IL25; interleukin 17E; interleukin 25; interleukin-17E; interleukin-25
IL17E; IL25; UNQ3120/PRO10272