|Tested species reactivity||Human|
|Published species reactivity||Dog, Pig, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||PHA activated T cells.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Structural elements necessary for oligomerization, trafficking, and cell sorting function of paraxial protocadherin.
AHR0024 was used in western blot to examine if Xenopus paraxial protocahedrin protein oligomerization and/or protein interactions are required for its cell sorting function
|Chen X,Molino C,Liu L,Gumbiner BM||The Journal of biological chemistry (282:32128)||2007|
|A dileucine motif targets the sulfate anion transporter sat-1 to the basolateral membrane in renal cell lines.||Regeer RR,Markovich D||American journal of physiology. Cell physiology (287:C365)||2004|
IL-12 decreases activation-induced cell death in human naive Th cells costimulated by intercellular adhesion molecule-1. I. IL-12 alters caspase processing and inhibits enzyme function.
AHR0024 was used in flow cytometry to study the regulatory role of IL-12 in ICAM-1-mediated costimulation.
|Palmer EM,Farrokh-Siar L,Maguire van Seventer J,van Seventer GA||Journal of immunology (Baltimore, Md. : 1950) (167:749)||2001|
Selective proteolytic cleavage of IL-2 receptor and IL-6 receptor ligand binding chains by neutrophil-derived serine proteases at foci of inflammation.
AHR0024 was used in flow cytometry to examine the role of role of neutrophil-derived serine proteases in traumatic brain injuries.
|Bank U,Reinhold D,Schneemilch C,Kunz D,Synowitz HJ,Ansorge S||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (19:1277)||1999|