|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat interleukin-2 receptor.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1/100|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Clone NDS61 recognizes a different epitope on CD25 than the OX39 clone. This antibody blocks IL-2 induced cell proliferation.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD25, also known as IL-2 receptor alpha, is a 55kDa type I membrane glycoprotein expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.