|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||T blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA5-17490 targets CD25/IL-2R alpha chain in FACS, FN, IHC (F), IHC (P), and IP applications and shows reactivity with Rat samples.
The MA5-17490 immunogen is t blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
CD8+ gammadelta T regulatory cells mediate kidney allograft prolongation after oral exposure to alloantigen.
MA5-17490 was used in flow cytometry to suggest that oral exposure to alloantigen results in IL-10 secreting, gammadelta TCR+ T cells
|Zhou J,Appleton SE,Stadnyk A,Lee TD,Nashan BA||Transplant international : official journal of the European Society for Organ Transplantation (21:679)||2008|