|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||B6.1 CTL Cell line|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.2 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 3 publications below|
MA5-17816 targets CD25/IL-2R alpha chain in FACS applications and shows reactivity with Mouse samples.
The MA5-17816 immunogen is b6.1 CTL Cell line.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of IgE production and airway reactivity by CD4¿CD8¿ regulatory T cells.
MA5-17816 was used in flow cytometry to characterize high zone tolerance in mouse models of type I allergy and of allergic airway inflammation.
|Raker V,Stein J,Montermann E,Maxeiner J,Taube C,Reske-Kunz AB,Sudowe S||Immunobiology (220:490)||2015|
Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.
MA5-17816 was used in flow cytometry to identify cells that mediate positive selection of CD8(+) T cells.
|Zerrahn J,Volkmann A,Coles MC,Held W,Lemonnier FA,Raulet DH||Proceedings of the National Academy of Sciences of the United States of America (96:11470)||1999|
Cellular and molecular characterization of the scurfy mouse mutant.
MA5-17816 was used in flow cytometry to further characterize mice hemizygous for scurfy.
|Clark LB,Appleby MW,Brunkow ME,Wilkinson JE,Ziegler SF,Ramsdell F||Journal of immunology (Baltimore, Md. : 1950) (162:2546)||1999|