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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Storage buffer||PBS with 4mg/ml BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 3 publications below|
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production.
RM6004 was used in flow cytometry to examine the T cell responses in cyclophosphamide-treated mice after systemic infection with Candida albicans.
|Angulo I,Jiménez-Díaz MB,García-Bustos JF,Gargallo D,de las Heras FG,Muñoz-Fernández MA,Fresno M||Cellular immunology (218:46)||2002|
Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+))CD11b(+ )immature myeloid suppressor cells.
RM6004 was used in flow cytometry to elucidate the immunosuppressive mechanism that occurs during T. cruzi infection.
|Goñi O,Alcaide P,Fresno M||International immunology (14:1125)||2002|
Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.
RM6004 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.
|Liu L,Rich BE,Inobe J,Chen W,Weiner HL||International immunology (10:1017)||1998|
IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA antibody; IL2-RA; Il2ra antibody; Interleukin 2 receptor alpha chain antibody; interleukin-2 receptor subunit alpha; p55 chain; TAC antigen antibody
CD25; Il2r; Il2ra; Ly-43