|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (210 QSYGAPRGDKEELTPQKC 227) of IL-32/Natural killer cells protein 4 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IL-32 is a 234 amino acid member of the cytokine family which plays an important role in innate and adaptive immune responses. It contains a tyrosine sulfation site, three potential N-myristoylation sites, multiple putative phosphorylation sites and a RGD cell-attachment sequence. IL-32 shows increased expression after activation of T lymphocytes and natural killer cells by IL-2 or mitogens. Human IL-32 exists as four splice variants. Reports suggest that it activates typical cytokine signal pathways of nuclear factor-kappa B (NF-kappaB) and p38 mitogen-activated protein kinase. It induces the production of various cytokines such as TNFA/TNF-alpha, macrophage inflammatory protein (MIP)-2 and IL-8, and has a possible role in inflammatory/autoimmune diseases like chronic obstructive pulmonary disease, Crohn and quote;s disease and psoriasis. It is ubiquitously expressed in most tissues with selective expression in lymphocytes and more prominently in immune cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.