|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 109–266 of mouse IL-33.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Interleukin 33 belongs to the IL-1 family of cytokines having dual mode of activity either as being secreted from cells to elicit their effects in an autocrine and paracrine manner or in an intracrine, nonsecreted manner as nuclear factors. IL-33 is produced by various tissue dwelling cells and is a crucial cytokine for Th2 cytokine-mediated host defense as well as induction of Th2-type allergic disorders. IL-33 has an additional role inside the cell, accumulating in the nucleus and associating with heterochromatin and mitotic chromatin and functioning as a transcriptional-repressor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.