Intracellular staining of Th2-polarized mouse splenocytes with Anti-Mouse CD4 APC-eFluor®780 and 0.125 ug of Rat IgG1 K Isotype Control APC (A18500, top) or 0.125 ug of Anti-Mouse IL-4 APC (bottom, A18500). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 were used for analysis.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||mouse interleukin 4|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
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