Sandwich ELISA analysis of mouse IL-4 was performed using a Mouse IL-4 Colorimetric ELISA kit (Product # EMIL4) by loading 50 ul per well of Mouse IL-4 Recombinant Protein (Product # SMIL4) in dodecuplicate at 375, 75, 15 and 0 pg/ml across a 2 ug/ml rat anti-mouse IL-4 (Product # MM450C) pre-coated plate and incubating for 2 hours at 37°C. The plate was washed, then incubated with 100 ul per well of an HRP-conjugated rat anti-mouse IL-4 antibody at a concentration of 0.4 ug/ml for 1 hour at 37°C. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Recombinant mouse IL-4|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM450C targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples. Does not bind to human IL-4.
The MM450C immunogen is recombinant mouse IL-4.
MM450C detects IL-4 which has a predicted molecular weight of approximately 14 kDa.
The MM450C mouse IL4 antibody (clone 1D11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM450DB (biotinylated conjugate of clone 24G2). Typical dilutions for sandwich ELISA include 1-5 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM450C (clone 1D11) and biotinylated antibody MM450DB (clone 24G2) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Treadmill exercise induces murine cardiac allograft survival and generates regulatory T cell.
MM450C was used in ELISA to analyze induction of murine cardiac allograft survival and regulatory T cells from treadmill exercise
|Uchiyama M,Jin X,Yin E,Shimokawa T,Niimi M||Transplant international : official journal of the European Society for Organ Transplantation (28:352)||2015|
|Not Applicable||Not Cited||
Inchingorei-san (TJ-117) and Artemisiae Capillaris Herba Induced Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice.
MM450C was used in ELISA to examine the effects of Inchingorei-san treatment on the alloimmune responses in a murine model of cardiac allograft transplantation
|Jin X,Uchiyama M,Zhang Q,Hirai T,Niimi M||Evidence-based complementary and alternative medicine : eCAM (2012:null)||2012|
|Not Applicable||Not Cited||
Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells.
MM450C was used in ELISA to assess the effect of sounds on alloimmune responses in a murine model of cardiac allograft transplantation
|Uchiyama M,Jin X,Zhang Q,Hirai T,Amano A,Bashuda H,Niimi M||Journal of cardiothoracic surgery (7:null)||2012|
|Not Applicable||10 µg/ml||
Induction of robust diabetes resistance and prevention of recurrent type 1 diabetes following islet transplantation by gene therapy.
MM450C was used in ELISA to determine whether the programmed death-1-programmed death ligand-1 pathway precipitates type 1 diabetes in young NOD mice
|Tian C,Ansari MJ,Paez-Cortez J,Bagley J,Godwin J,Donnarumma M,Sayegh MH,Iacomini J||Journal of immunology (Baltimore, Md. : 1950) (179:6762)||2007|
ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.
MM450C was used in ELISA to investigate the involvement of pollen NADPH oxidase in allergic airway inflammation
|Boldogh I,Bacsi A,Choudhury BK,Dharajiya N,Alam R,Hazra TK,Mitra S,Goldblum RM,Sur S||The Journal of clinical investigation (115:2169)||2005|
Stimulation of interleukin-10 production by acidic beta-lactoglobulin-derived peptides hydrolyzed with Lactobacillus paracasei NCC2461 peptidases.
MM450C was used in ELISA to investigate the induction of interleukin-10 production by acidic beta-lactoglobulin-derived peptides
|Prioult G,Pecquet S,Fliss I||Clinical and diagnostic laboratory immunology (11:266)||2004|
|Not Applicable||Not Cited||
Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen.
MM450C was used in ELISA to determine the roles of PD-1 and its ligands in the induction of regulatory cells by intratracheal delivery of alloantigen
|Aramaki O,Shirasugi N,Takayama T,Shimazu M,Kitajima M,Ikeda Y,Azuma M,Okumura K,Yagita H,Niimi M||Transplantation (77:6)||2004|
Enhanced mucosal and systemic immune responses to Helicobacter pylori antigens through mucosal priming followed by systemic boosting immunizations.
MM450C was used in ELISA to detect anti-CagA and anti-NAP cytokine-secreting cells
|Vajdy M,Singh M,Ugozzoli M,Briones M,Soenawan E,Cuadra L,Kazzaz J,Ruggiero P,Peppoloni S,Norelli F,del Giudice G,O'Hagan D||Immunology (110:86)||2003|
The levels and patterns of cytokines produced by CD4 T lymphocytes of BALB/c mice infected with Leishmania major by inoculation into the ear dermis depend on the infectiousness and size of the inoculum.
MM450C was used in ELISA to investigate the cytokine profile after Leishmania major inoculation
|Lang T,Courret N,Colle JH,Milon G,Antoine JC||Infection and immunity (71:2674)||2003|
Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.
MM450C was used in ELISA to study the vaccination against respiratory syncytial virus
|Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P||Journal of immunology (Baltimore, Md. : 1950) (169:3208)||2002|
The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.
MM450C was used in ELISA to investigate the effect of 3O-C12-HSL for inflammation induction in mice
|Smith RS,Harris SG,Phipps R,Iglewski B||Journal of bacteriology (184:1132)||2002|
The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.
MM450C was used in ELISA to study the interleukin levels in the mice with various diets
|Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA||Surgery (131:59)||2002|
Murine CD4+ T lymphocyte subsets and host defense against Pneumocystis carinii.
MM450C was used in ELISA to investigate the murine immunoresponse against Pneumocystis carinii
|Shellito JE,Tate C,Ruan S,Kolls J||The Journal of infectious diseases (181:2011)||2000|
Oral administration of chitin down-regulates serum IgE levels and lung eosinophilia in the allergic mouse.
MM450C was used in ELISA to investigate the effect of chitin administration on serum IgE levels and lung eosinophilia
|Shibata Y,Foster LA,Bradfield JF,Myrvik QN||Journal of immunology (Baltimore, Md. : 1950) (164:1314)||2000|
Repeated administration of synthetic oligodeoxynucleotides expressing CpG motifs provides long-term protection against bacterial infection.
MM450C was used in ELISA to investigate the protective effect of synthetic oligodeoxynucleotides against bacterial infection
|Klinman DM,Conover J,Coban C||Infection and immunity (67:5658)||1999|
Mice with STAT6-targeted gene disruption develop a Th1 response and control cutaneous leishmaniasis.
MM450C was used in ELISA to investigate the effect of STAT6 for a Th1-like response and on cutaneous L. mexicana infection in mice
|Stamm LM,Räisänen-Sokolowski A,Okano M,Russell ME,David JR,Satoskar AR||Journal of immunology (Baltimore, Md. : 1950) (161:6180)||1998|
Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.
MM450C was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses
|Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH||Journal of immunology (Baltimore, Md. : 1950) (161:4146)||1998|
Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
MM450C was used in ELISA to study the mechanism for T cell differentiation
|Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A||Journal of immunology (Baltimore, Md. : 1950) (161:2825)||1998|
Sex-determined resistance against Leishmania mexicana is associated with the preferential induction of a Th1-like response and IFN-gamma production by female but not male DBA/2 mice.
MM450C was used in ELISA to study the sex-specific immune resistance against Leishmania mexicana and its mechanism
|Satoskar A,Al-Quassi HH,Alexander J||Immunology and cell biology (76:159)||1998|
Induction of neonatal tolerance by plasmid DNA vaccination of mice.
MM450C was used in ELISA to investigate the induction of neonatal tolerance by plasmid DNA vaccination of mice
|Mor G,Yamshchikov G,Sedegah M,Takeno M,Wang R,Houghten RA,Hoffman S,Klinman DM||The Journal of clinical investigation (98:2700)||1996|
Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.
MM450C was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation
|Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E||Journal of immunology (Baltimore, Md. : 1950) (157:566)||1996|
Down-regulation of Th2 cell-mediated murine peritoneal eosinophilia by antiallergic agents.
MM450C was used in ELISA to study the role of antiallergic agents in TH2 cell-mediated murine peritoneal eosinophilia
|Yamaya H,Basaki Y,Togawa M,Kojima M,Kiniwa M,Matsuura N||Life sciences (56:1647)||1995|
T-helper type-1-dominated lymph node responses induced in C57BL/6 mice by optimally irradiated cercariae of Schistosoma mansoni are down-regulated after challenge infection.
MM450C was used in ELISA to investigate the immune response against Schistosoma mansoni
|Pemberton RM,Wilson RA||Immunology (84:310)||1995|
Topical FK506 suppresses cytokine and costimulatory molecule expression in epidermal and local draining lymph node cells during primary skin immune responses.
MM450C was used in flow cytometry to investigate the changes of gene expression by FK506 stimulation during primary skin immune responses
|Homey B,Assmann T,Vohr HW,Ulrich P,Lauerma AI,Ruzicka T,Lehmann P,Schuppe HC||Journal of immunology (Baltimore, Md. : 1950) (160:5331)||1998|
Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.
MM450C was used in blocking/activating experiment to investigate the role of interleukin 12 in Th1 cell function
|Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G||European journal of immunology (26:659)||1996|
Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes.
MM450C was used in immunocytochemistry to study the influence of anti-cytokine antibodies on the therapeutic effect of cytokine injection
|Finkelman FD,Madden KB,Morris SC,Holmes JM,Boiani N,Katona IM,Maliszewski CR||Journal of immunology (Baltimore, Md. : 1950) (151:1235)||1993|