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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Protein corresponding to amino acids 25–153 of human IL-4|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||4-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Interleukin-4 is a pleiotropic cytokine responsible for differentiation of T-cells. IL-4 has been found to have the broadest range of actions. It has been found to bind to IL-13 which may result in overlapping functions between IL-4 and IL-13. A signal transducer STAT6 is known to play a important role in arbitrating immune regulatory signal of IL-4. It is found as a cluster with other Interleukins on the q arm of chromosome 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; BSF-1; interleukin 4 variant 2; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra
BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4