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|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant mouse IL-4|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM450D targets IL-4 in WB applications and shows reactivity with mouse samples.
The MM450D immunogen is recombinant mouse IL-4.
MM450D detects IL-4 which has a predicted molecular weight of approximately 14 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.
MM450D was used in ELISA to investigate the involvement of pollen NADPH oxidase in allergic airway inflammation
|Boldogh I,Bacsi A,Choudhury BK,Dharajiya N,Alam R,Hazra TK,Mitra S,Goldblum RM,Sur S||The Journal of clinical investigation (115:2169)||2005|
T cytotoxic-1 CD8+ T cells are effector cells against pneumocystis in mice.
MM450D was used in ELISA to investigate the role of T cytotoxic-1 CD8+ T cells in protection again pneumocystis
|McAllister F,Mc Allister F,Steele C,Zheng M,Young E,Shellito JE,Marrero L,Kolls JK||Journal of immunology (Baltimore, Md. : 1950) (172:1132)||2004|
The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo.
MM450D was used in ELISA to investigate the effect of 3O-C12-HSL for inflammation induction in mice
|Smith RS,Harris SG,Phipps R,Iglewski B||Journal of bacteriology (184:1132)||2002|
Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice.
MM450D was used in ELISA to characterize the airway immune response after influenza A virus infection
|Yamamoto N,Suzuki S,Suzuki Y,Shirai A,Nakazawa M,Suzuki M,Takamasu T,Nagashima Y,Minami M,Ishigatsubo Y||Journal of virology (75:499)||2001|
Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.
MM450D was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses
|Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH||Journal of immunology (Baltimore, Md. : 1950) (161:4146)||1998|
Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
MM450D was used in ELISA to study the mechanism for T cell differentiation
|Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A||Journal of immunology (Baltimore, Md. : 1950) (161:2825)||1998|
Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.
MM450D was used in ELISA to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells
|DeKruyff RH,Fang Y,Umetsu DT||Journal of immunology (Baltimore, Md. : 1950) (160:2231)||1998|
Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.
MM450D was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation
|Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E||Journal of immunology (Baltimore, Md. : 1950) (157:566)||1996|
Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.
MM450D was used in ELISA to investigate the role of interleukin 12 in Th1 cell function
|Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G||European journal of immunology (26:659)||1996|
Down-regulation of Th2 cell-mediated murine peritoneal eosinophilia by antiallergic agents.
MM450D was used in ELISA to study the role of antiallergic agents in TH2 cell-mediated murine peritoneal eosinophilia
|Yamaya H,Basaki Y,Togawa M,Kojima M,Kiniwa M,Matsuura N||Life sciences (56:1647)||1995|
IgE receptor-positive non-B/non-T cells dominate the production of interleukin 4 and interleukin 6 in immunized mice.
MM450D was used in ELISA to study the roles of IgE receptor-positive non-B/non-T cells in interleukin 4 and interleukin 6 production during immunization
|Aoki I,Kinzer C,Shirai A,Paul WE,Klinman DM||Proceedings of the National Academy of Sciences of the United States of America (92:2534)||1995|
CD40-mediated stimulation contributes to lymphocyte proliferation, antibody production, eosinophilia, and mastocytosis during an in vivo type 2 response, but is not required for T cell IL-4 production.
MM450D was used in blocking/activating experiment to study the role of CD40 in immune response
|Lu P,Urban JF,Zhou XD,Chen SJ,Madden K,Moorman M,Nguyen H,Morris SC,Finkelman FD,Gause WC||Journal of immunology (Baltimore, Md. : 1950) (156:3327)||1996|
Anti-cytokine antibodies as carrier proteins. Prolongation of in vivo effects of exogenous cytokines by injection of cytokine-anti-cytokine antibody complexes.
MM450D was used in immunocytochemistry to study the influence of anti-cytokine antibodies on the therapeutic effect of cytokine injection
|Finkelman FD,Madden KB,Morris SC,Holmes JM,Boiani N,Katona IM,Maliszewski CR||Journal of immunology (Baltimore, Md. : 1950) (151:1235)||1993|
Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.
MM450D was used in immunohistochemistry to investigate the production of interleukin 2 and 4 and interferon gamma in mice immunized with protein antigens
|Bogen SA,Fogelman I,Abbas AK||Journal of immunology (Baltimore, Md. : 1950) (150:4197)||1993|
B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; interleukin-4; lymphocyte stimulatory factor 1
BSF-1; Il-4; Il4