|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant mouse IL-4|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 17 publications below|
MM450DB targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples.
The MM450DB immunogen is recombinant mouse IL-4.
MM450DB detects IL-4 which has a predicted molecular weight of approximately 14 kDa.
The MM450DB mouse IL4 antibody (biotinylated conjugate of clone 24G2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM450C (clone 1D11). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody MM450DB (clone 24G2) and antibody MM450C (clone 1D11) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation.
MM450DB was used in ELISA to investigate the involvement of pollen NADPH oxidase in allergic airway inflammation
|Boldogh I,Bacsi A,Choudhury BK,Dharajiya N,Alam R,Hazra TK,Mitra S,Goldblum RM,Sur S||The Journal of clinical investigation (115:2169)||2005|
Stimulation of interleukin-10 production by acidic beta-lactoglobulin-derived peptides hydrolyzed with Lactobacillus paracasei NCC2461 peptidases.
MM450DB was used in ELISA to investigate the induction of interleukin-10 production by acidic beta-lactoglobulin-derived peptides
|Prioult G,Pecquet S,Fliss I||Clinical and diagnostic laboratory immunology (11:266)||2004|
T cytotoxic-1 CD8+ T cells are effector cells against pneumocystis in mice.
MM450DB was used in ELISA to investigate the role of T cytotoxic-1 CD8+ T cells in protection again pneumocystis
|McAllister F,Mc Allister F,Steele C,Zheng M,Young E,Shellito JE,Marrero L,Kolls JK||Journal of immunology (Baltimore, Md. : 1950) (172:1132)||2004|
Enhanced mucosal and systemic immune responses to Helicobacter pylori antigens through mucosal priming followed by systemic boosting immunizations.
MM450DB was used in ELISA to detect anti-CagA and anti-NAP cytokine-secreting cells
|Vajdy M,Singh M,Ugozzoli M,Briones M,Soenawan E,Cuadra L,Kazzaz J,Ruggiero P,Peppoloni S,Norelli F,del Giudice G,O'Hagan D||Immunology (110:86)||2003|
The levels and patterns of cytokines produced by CD4 T lymphocytes of BALB/c mice infected with Leishmania major by inoculation into the ear dermis depend on the infectiousness and size of the inoculum.
MM450DB was used in ELISA to investigate the cytokine profile after Leishmania major inoculation
|Lang T,Courret N,Colle JH,Milon G,Antoine JC||Infection and immunity (71:2674)||2003|
Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.
MM450DB was used in ELISA to study the vaccination against respiratory syncytial virus
|Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P||Journal of immunology (Baltimore, Md. : 1950) (169:3208)||2002|
The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.
MM450DB was used in ELISA to study the interleukin levels in the mice with various diets
|Zarzaur BL,Wu Y,Fukatsu K,Johnson CD,Kudsk KA||Surgery (131:59)||2002|
Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice.
MM450DB was used in ELISA to characterize the airway immune response after influenza A virus infection
|Yamamoto N,Suzuki S,Suzuki Y,Shirai A,Nakazawa M,Suzuki M,Takamasu T,Nagashima Y,Minami M,Ishigatsubo Y||Journal of virology (75:499)||2001|
IFN-gamma-inducing factor (IL-18) increases allergic sensitization, serum IgE, Th2 cytokines, and airway eosinophilia in a mouse model of allergic asthma.
MM450DB was used in ELISA to study the effect of interleukin-18 on allergic responses in a mouse asthma model
|Wild JS,Sigounas A,Sur N,Siddiqui MS,Alam R,Kurimoto M,Sur S||Journal of immunology (Baltimore, Md. : 1950) (164:2701)||2000|
Mice with STAT6-targeted gene disruption develop a Th1 response and control cutaneous leishmaniasis.
MM450DB was used in ELISA to investigate the effect of STAT6 for a Th1-like response and on cutaneous L. mexicana infection in mice
|Stamm LM,Räisänen-Sokolowski A,Okano M,Russell ME,David JR,Satoskar AR||Journal of immunology (Baltimore, Md. : 1950) (161:6180)||1998|
Heat-killed Listeria monocytogenes as an adjuvant converts established murine Th2-dominated immune responses into Th1-dominated responses.
MM450DB was used in ELISA to investigate the effect of Listeria monocytogenes on immune responses
|Yeung VP,Gieni RS,Umetsu DT,DeKruyff RH||Journal of immunology (Baltimore, Md. : 1950) (161:4146)||1998|
Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4.
MM450DB was used in ELISA to study the mechanism for T cell differentiation
|Assenmacher M,Löhning M,Scheffold A,Richter A,Miltenyi S,Schmitz J,Radbruch A||Journal of immunology (Baltimore, Md. : 1950) (161:2825)||1998|
Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.
MM450DB was used in ELISA to investigate the effect of phosphatidylcholine immunization of mice for the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections
|Bordmann G,Rudin W,Favre N||Immunology (94:35)||1998|
Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.
MM450DB was used in ELISA to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells
|DeKruyff RH,Fang Y,Umetsu DT||Journal of immunology (Baltimore, Md. : 1950) (160:2231)||1998|
Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.
MM450DB was used in ELISA to investigate the effect of CD4 antibodies on Th1 differentiation
|Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E||Journal of immunology (Baltimore, Md. : 1950) (157:566)||1996|
Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.
MM450DB was used in ELISA to investigate the role of interleukin 12 in Th1 cell function
|Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G||European journal of immunology (26:659)||1996|
T-helper type-1-dominated lymph node responses induced in C57BL/6 mice by optimally irradiated cercariae of Schistosoma mansoni are down-regulated after challenge infection.
MM450DB was used in ELISA to investigate the immune response against Schistosoma mansoni
|Pemberton RM,Wilson RA||Immunology (84:310)||1995|