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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant human IL-4 (CHO cell-derived)|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
M450 targets IL-4 in WB applications and shows reactivity with Human samples.
The M450 immunogen is recombinant human IL-4 (CHO cell-derived).
M450 detects IL-4 which has a predicted molecular weight of approximately 15 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of interleukin-4 signaling by extracellular reduction of intramolecular disulfides.
M450 was used in ELISA to study the effect of disulfide bonds on interleukin-4 signaling
|Curbo S,Gaudin R,Carlsten M,Malmberg KJ,Troye-Blomberg M,Ahlborg N,Karlsson A,Johansson M,Lundberg M||Biochemical and biophysical research communications (390:1272)||2009|
Epstein-Barr virus and cytomegalovirus are differentially associated with numbers of cytokine-producing cells and early atopy.
M450 was used in ELISA to investigate the relationship between the cytokine profile in PBMC and Epstein-Barr virus serostatus and between the cytokine profile and cytomegalovirus serostatus
|Nilsson C,Larsson Sigfrinius AK,Montgomery SM,Sverremark-Ekström E,Linde A,Lilja G,Blomberg MT||Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (39:509)||2009|
Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.
M450 was used in ELISA to study the mucosal production of interleukin 13 in Helicobacter pylori-related gastric diseases
|Marotti B,Rocco A,De Colibus P,Compare D,de Nucci G,Staibano S,Tatangelo F,Romano M,Nardone G||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (40:240)||2008|
Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.
M450 was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients
|Loffreda S,Muratori P,Muratori L,Mele L,Bianchi FB,Lenzi M||Journal of hepatology (38:230)||2003|
Influence of atopic heredity on IL-4-, IL-12- and IFN-gamma-producing cells in in vitro activated cord blood mononuclear cells.
M450 was used in ELISA to study the effect of fetal environment on the development of atopic disease
|Gabrielsson S,Söderlund A,Nilsson C,Lilja G,Nordlund M,Troye-Blomberg M||Clinical and experimental immunology (126:390)||2001|
Antigen-independent Th2 cell differentiation by stimulation of CD28: regulation via IL-4 gene expression and mitogen-activated protein kinase activation.
M450 was used in ELISA to investigate the role of interleukin 4 gene expression
|Skapenko A,Lipsky PE,Kraetsch HG,Kalden JR,Schulze-Koops H||Journal of immunology (Baltimore, Md. : 1950) (166:4283)||2001|
Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression.
M450 was used in ELISA to measure the interleukin-10 and interleukin-4 level in Tanzanian children with malaria
|Anstey NM,Weinberg JB,Hassanali MY,Mwaikambo ED,Manyenga D,Misukonis MA,Arnelle DR,Hollis D,McDonald MI,Granger DL||The Journal of experimental medicine (184:557)||1996|
Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis.
M450 was used in immunohistochemistry to study the involvement of interleukin 10 in active ulcerative colitis
|Melgar S,Yeung MM,Bas A,Forsberg G,Suhr O,Oberg A,Hammarstrom S,Danielsson A,Hammarstrom ML||Clinical and experimental immunology (134:127)||2003|
Restriction of de novo pyrimidine biosynthesis inhibits Th1 cell activation and promotes Th2 cell differentiation.
M450 was used in blocking/activating experiment to investigate the role of de novo pyrimidine biosynthesis restriction for Th1 cell activation and Th2 cell differentiation
|Dimitrova P,Skapenko A,Herrmann ML,Schleyerbach R,Kalden JR,Schulze-Koops H||Journal of immunology (Baltimore, Md. : 1950) (169:3392)||2002|
B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; BSF-1; interleukin 4 variant 2; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra
BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4